FastQCFastQC Report
Wed 25 May 2016
SRR1295062_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295062_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2934164
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC164130.5593756858853152No Hit
CCCATGTACTCTGCGTTGATACCAC145020.49424640204160364No Hit
GAGTACATGGGAAGCAGTGGTATCA82840.2823291404297783No Hit
CATGTACTCTGCGTTGATACCACTG61400.20925892349575553No Hit
GCGTTGATACCACTGCTTCCCATGT47210.1608976185380231No Hit
GCTTCCCATGTACTCTGCGTTGATA46810.15953436822208983No Hit
ACGCAGAGTACATGGGAAGCAGTGG43560.1484579594051321No Hit
GTATCAACGCAGAGTACTTTTTTTT43470.1481512280840471No Hit
GTATCAACGCAGAGTACATGGGAAG42030.14324352694668738No Hit
GGTATCAACGCAGAGTACATGGGAA40360.137551956877666No Hit
GGTATCAACGCAGAGTACTTTTTTT39800.13564340643535944No Hit
TATCAACGCAGAGTACATGGGAAGC36720.12514637900267334No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN34460.11744401471765042No Hit
ACTCTGCGTTGATACCACTGCTTCC33350.11366099509093561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTC751.4818843E-512.66488
GCACCGT803.779157E-410.6855596
CGCGATC1106.0717393E-610.36051617
GCGCGAT1201.530525E-610.28874316
TTACACC3250.010.2366054
CGTTCAA750.002652383610.13166610
ATACCGT750.002652759710.1314946
GGCGTGC856.605599E-410.0573418
GTCTTAC2650.09.6915581
CTTACAC3550.09.63880353
ATAGGGC2002.6193447E-109.5049313
CCAATAC2800.09.50493053
TACACCG1106.8040565E-59.5046065
GTGTAAG5100.09.3255741
TCTGTCG2151.07320375E-109.2777018
TGGCGCG1451.7136645E-69.16996514
GGCGCGA1356.8777754E-69.14539315
CTAATAC3850.09.1346093
AGTACTG3550.09.10300355
GTGCTAG3350.09.0861711
Produced by FastQC (version 0.11.5)