FastQCFastQC Report
Wed 25 May 2016
SRR1295062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295062_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2934164
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC163870.5584895731799586No Hit
CCCATGTACTCTGCGTTGATACCAC161490.5503782338001557No Hit
GTATCAACGCAGAGTACTTTTTTTT120240.4097930449695382No Hit
GGTATCAACGCAGAGTACTTTTTTT114520.39029856545169256No Hit
GAGTACATGGGAAGCAGTGGTATCA91310.3111959658696651No Hit
TATCAACGCAGAGTACTTTTTTTTT83390.28420360961418656No Hit
CATGTACTCTGCGTTGATACCACTG68440.23325212905618092No Hit
ACGCAGAGTACATGGGAAGCAGTGG53800.18335716749302355No Hit
GCTTCCCATGTACTCTGCGTTGATA53500.18233472975607362No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51860.17674540346074727No Hit
GCGTTGATACCACTGCTTCCCATGT50550.17228075867606582No Hit
GTATCAACGCAGAGTACATGGGAAG46070.1570123551376133No Hit
GGTATCAACGCAGAGTACATGGGAA42610.1452202399047906No Hit
TATCAACGCAGAGTACATGGGAAGC42250.14399331462045067No Hit
ACTCTGCGTTGATACCACTGCTTCC37220.1268504418975899No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG550.00307115112.0900745
AGACCGT951.041677E-611.999176
GCGTCAG1104.9777736E-711.2264984
TAGGACT2850.010.999244
GCAGCGT1105.962045E-610.3763591
AGCGTCA1201.5263122E-610.29095653
GTTCTAG3000.09.51166251
CGTCAGA1304.245241E-69.4993445
CTAGGAC2800.09.4993443
TATACTG6800.09.3596485
TCTAGAC2700.09.1475163
GGCGCCC1151.1120189E-49.0863298
GTCTTAG2800.08.8322581
CAGCGTC1401.0864382E-58.820972
GTCCTAG3050.08.7320181
GTCTTAA3050.08.7320181
TAGGAGT3700.08.7291274
ACCGTGT2201.6079866E-98.6357678
TACACTG7650.08.4438625
CGTGTTA1356.8066656E-58.44386110
Produced by FastQC (version 0.11.5)