FastQCFastQC Report
Wed 25 May 2016
SRR1295061_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295061_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2430711
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC77900.3204823609223803No Hit
CCCATGTACTCTGCGTTGATACCAC68660.28246879205302483No Hit
GAGTACATGGGAAGCAGTGGTATCA40420.1662887936904058No Hit
GTATCAACGCAGAGTACTTTTTTTT36910.1518485743471766No Hit
GGTATCAACGCAGAGTACTTTTTTT34160.14053501218367795No Hit
CATGTACTCTGCGTTGATACCACTG29040.11947121644654589No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN28160.11585087655422632No Hit
ATTCCATTCCATTCCATTCCATTCC26760.11009124490735425No Hit
TATCAACGCAGAGTACTTTTTTTTT24620.10128723653284985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG400.00525488514.260345
TATTCCG1051.9752406E-812.6758565
CCAAGCG550.003071593612.0897029
CAATGCG600.00588163711.0822289
AGCTCCG1054.1131596E-59.9509766
ATTAGAC2251.8189894E-129.7169533
GCTACAC1201.689018E-59.5057153
CTAGGAC2303.6379788E-129.5057153
CTAATAC3300.09.5057153
CTTACAC2900.09.5057153
GCTCCGC900.00111521519.4988567
CGGGTTC1502.6629277E-79.49670118
GCGCGAT900.00111816289.49611516
CTAGACA3050.09.3514274
GTCCTAA2252.5465852E-119.3002351
GTATTAG3400.09.2318511
GTTCTAG2501.8189894E-129.131141
GTCCTAG2201.6370905E-109.0792581
GTCTAAC1054.439262E-49.058671
CGCGATC1054.5121732E-49.04391917
Produced by FastQC (version 0.11.5)