FastQCFastQC Report
Wed 25 May 2016
SRR1295061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295061_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2430711
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT106060.43633323747660663No Hit
GGTATCAACGCAGAGTACTTTTTTT103370.4252665166694025No Hit
GTACATGGGAAGCAGTGGTATCAAC77580.31916587368880955No Hit
CCCATGTACTCTGCGTTGATACCAC76200.3134885224940357No Hit
TATCAACGCAGAGTACTTTTTTTTT72650.29888374224661013No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45420.18685890671494884No Hit
GAGTACATGGGAAGCAGTGGTATCA43700.17978278783450605No Hit
CATGTACTCTGCGTTGATACCACTG31670.13029109589745552No Hit
ACGCAGAGTACATGGGAAGCAGTGG26320.10828107496119448No Hit
GCTTCCCATGTACTCTGCGTTGATA26030.10708800840577097No Hit
ATTCCATTCCATTCCATTCCATTCC24940.1026037237664206No Hit
GCGTTGATACCACTGCTTCCCATGT24460.10062899291606447No Hit
GGTATCAACGCAGAGTACATGGGAA24340.10013531020347545No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT550.003071094412.0899678
GTTCTAG2750.010.3779861
CCCTAAC1606.037226E-910.1077251
TAGGACT2450.010.0808474
GGAACGA951.6459833E-49.99922115
TAGACAG3450.09.3615895
GTCTAGA2451.8189894E-129.3190071
ATAGGAC1856.710252E-99.2425233
GGTATAG1651.04369974E-79.2248751
GTCATAG2600.09.1472631
GTCTTAC1901.0335498E-89.0124611
CCGGGTT1804.2256943E-88.97152217
CCGGGAC850.00744203348.940483
CTAAGGT1701.6876038E-78.940484
CGGGTTC1606.750779E-78.90555618
TTAGCAC2252.6193447E-108.8659753
GTCTTGG2800.08.8336421
ACCGTGT2054.147296E-98.8041928
GTAGGAC1201.765757E-48.7076543
CGTGTTA1201.765757E-48.70765410
Produced by FastQC (version 0.11.5)