FastQCFastQC Report
Wed 25 May 2016
SRR1295060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3451991
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC297510.8618504509426589No Hit
CCCATGTACTCTGCGTTGATACCAC267500.7749151142051065No Hit
GAGTACATGGGAAGCAGTGGTATCA151040.4375445938300534No Hit
CATGTACTCTGCGTTGATACCACTG115930.33583517454130096No Hit
GCTTCCCATGTACTCTGCGTTGATA86670.25107249700245454No Hit
GCGTTGATACCACTGCTTCCCATGT82860.2400353882730285No Hit
ACGCAGAGTACATGGGAAGCAGTGG76950.22291483378722599No Hit
TATCAACGCAGAGTACATGGGAAGC68790.19927630170530572No Hit
GGTATCAACGCAGAGTACATGGGAA64400.18655900319554716No Hit
GTATCAACGCAGAGTACATGGGAAG62310.1805045262284867No Hit
ACTCTGCGTTGATACCACTGCTTCC61700.17873743008020587No Hit
GTATCAACGCAGAGTACTTTTTTTT40600.11761328462327972No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN40410.11706287762627422No Hit
GGTATCAACGCAGAGTACTTTTTTT37830.1095889299827259No Hit
GTGGTATCAACGCAGAGTACATGGG34750.10066654287337366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGC250.0060382518.997436
TGGACCG551.9548055E-413.8235375
AACCGTG604.097439E-412.6655057
GGACCGT951.0426829E-611.9983776
TTCCGTA1251.8369974E-811.3989557
CTGTCGC2850.010.9994719
TAAGCGT700.001486663910.8619814
GACCGTG750.002650985510.1324047
ACCGTGG856.6002004E-410.058348
TCTGTCG3100.09.805988
AGGACCG1106.812238E-59.5036825
TATTCCG1502.636225E-79.5036825
CCGTCCT1002.7583566E-49.4995429
GCACCGT900.00111561699.4987146
CTAGACA4800.09.306234
TAGACAG5100.09.1309895
TAGGACC2201.6734703E-109.0722244
TATACTG8700.09.0667315
GTTACAC2106.6393113E-109.0509943
TCCTACG850.00741586658.9445122
Produced by FastQC (version 0.11.5)