FastQCFastQC Report
Wed 25 May 2016
SRR1295060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3451991
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC294210.8522907504683529No Hit
CCCATGTACTCTGCGTTGATACCAC291500.8444402085636956No Hit
GAGTACATGGGAAGCAGTGGTATCA162160.46975788754953296No Hit
CATGTACTCTGCGTTGATACCACTG127300.3687726879936825No Hit
GGTATCAACGCAGAGTACTTTTTTT105700.3062001030709524No Hit
GTATCAACGCAGAGTACTTTTTTTT104730.30339013050729274No Hit
ACGCAGAGTACATGGGAAGCAGTGG97270.281779413677498No Hit
GCTTCCCATGTACTCTGCGTTGATA94430.27355227751173167No Hit
TATCAACGCAGAGTACTTTTTTTTT89630.2596472586400138No Hit
GCGTTGATACCACTGCTTCCCATGT86780.25139115368493137No Hit
TATCAACGCAGAGTACATGGGAAGC73990.2143400721496667No Hit
GGTATCAACGCAGAGTACATGGGAA70350.203795432838614No Hit
ACTCTGCGTTGATACCACTGCTTCC68720.1990735201800932No Hit
GTATCAACGCAGAGTACATGGGAAG67450.1953944839369512No Hit
ACGCAGAGTACTTTTTTTTTTTTTT50630.1466689803073067No Hit
GTGGTATCAACGCAGAGTACATGGG39540.11454259295577537No Hit
CAGTGGTATCAACGCAGAGTACATG39360.11402115474808597No Hit
ACATGGGAAGCAGTGGTATCAACGC37560.10880677267119179No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACT600.005880758611.0826654
CGCAAAA909.5235024E-510.5553792
ACCGCGC1355.7012585E-810.55476718
CACCGCG1355.7012585E-810.55476717
ACGACTG750.002650295410.1327235
CAGTGCG856.6018215E-410.0580739
GTGCTAG2950.09.9954811
CTAGGAC4000.09.7369133
TAGACAG6400.09.6478575
GGAACGA1106.846781E-59.49929115
GTGTAGG3950.09.3914251
CCGCGCC1554.2693318E-79.19286219
CGAGTAC1151.1120634E-49.0864093
ACAACGT1151.1122388E-49.0862778
GTCTAAG3200.08.9173381
GAACTGT4400.08.8516126
TAGACTG4850.08.8139025
CCTAGAC3450.08.8110643
TAGGGCT3050.08.7207864
CTAGTAC2304.0563464E-108.673393
Produced by FastQC (version 0.11.5)