FastQCFastQC Report
Wed 25 May 2016
SRR1295059_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295059_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998095
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC155490.5186293296243114No Hit
CCCATGTACTCTGCGTTGATACCAC133700.4459498448181262No Hit
GAGTACATGGGAAGCAGTGGTATCA79080.26376749235764707No Hit
CATGTACTCTGCGTTGATACCACTG56930.18988724506728438No Hit
GTATCAACGCAGAGTACTTTTTTTT51200.17077510886079325No Hit
GCGTTGATACCACTGCTTCCCATGT45040.15022872857597908No Hit
GCTTCCCATGTACTCTGCGTTGATA44580.1486944209573079No Hit
GGTATCAACGCAGAGTACTTTTTTT44550.14859435741695978No Hit
GTATCAACGCAGAGTACATGGGAAG41170.13732053187107146No Hit
ACGCAGAGTACATGGGAAGCAGTGG40120.13381830795888724No Hit
GGTATCAACGCAGAGTACATGGGAA39290.1310498833425892No Hit
TATCAACGCAGAGTACATGGGAAGC35640.11887548593356781No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN35620.11880877690666908No Hit
TATCAACGCAGAGTACTTTTTTTTT34580.11533990750793421No Hit
ACTCTGCGTTGATACCACTGCTTCC30710.10243171080302658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG3800.010.2556325
GCCTTAG2400.09.9082331
TAGACAG4200.09.7315345
GACTGTC2054.1836756E-119.7300557
GTCTAAC1701.6327249E-89.5119041
TAGGCGT800.00449460479.5058564
ATTGGAC1401.0517433E-69.5050613
CTAGGGC1951.6680133E-99.2613413
TAGGGCC1752.6455382E-89.234264
ACAGTCC3000.09.1820818
AGGCGTG4350.09.17085557
TCAGTAC2304.1836756E-119.0917983
TAACCCG950.00181538699.0049465
CCAGTAC3850.08.887853
AGTACTC2900.08.8496885
TATACTG7950.08.8476275
GCTAGAC1951.6354534E-88.7739023
TAGGACA3600.08.7137014
CCTATAC2850.08.6712833
CTAGACT3100.08.5859354
Produced by FastQC (version 0.11.5)