##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295059_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2998095
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2501675230438	33.0	33.0	33.0	27.0	33.0
2	31.125352932445438	33.0	33.0	33.0	27.0	33.0
3	31.214082942668593	33.0	33.0	33.0	27.0	33.0
4	34.79599779193121	37.0	37.0	37.0	27.0	37.0
5	35.021367568405935	37.0	37.0	37.0	33.0	37.0
6	35.05136861907311	37.0	37.0	37.0	33.0	37.0
7	34.88784044534946	37.0	37.0	37.0	33.0	37.0
8	34.83157538370198	37.0	37.0	37.0	33.0	37.0
9	34.71104851580754	37.0	37.0	37.0	27.0	37.0
10	34.62340586272283	37.0	37.0	37.0	27.0	37.0
11	34.815033879847036	37.0	37.0	37.0	33.0	37.0
12	34.72507709061921	37.0	37.0	37.0	27.0	37.0
13	34.6502158870883	37.0	37.0	37.0	27.0	37.0
14	36.044809787548424	40.0	37.0	40.0	27.0	40.0
15	36.22062476339142	40.0	37.0	40.0	27.0	40.0
16	36.30927138733095	40.0	37.0	40.0	27.0	40.0
17	36.23746545723201	40.0	37.0	40.0	27.0	40.0
18	36.174314356282906	40.0	37.0	40.0	27.0	40.0
19	36.28711798658815	40.0	37.0	40.0	27.0	40.0
20	36.284539015608246	40.0	37.0	40.0	27.0	40.0
21	36.2930450836281	40.0	37.0	40.0	27.0	40.0
22	36.19392314119466	40.0	37.0	40.0	27.0	40.0
23	36.10742521501153	40.0	37.0	40.0	27.0	40.0
24	36.16554045152005	40.0	37.0	40.0	27.0	40.0
25	35.057671287934504	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.438187767702253
1101	2	0.4144191054310724
1101	3	0.43530868519983557
1101	4	0.5363537853936933
1101	5	0.3586752360319352
1101	6	0.25043353272184987
1101	7	0.2782996065429231
1101	8	0.4306655808834492
1101	9	0.4470777578085219
1101	10	0.5786101651770466
1101	11	0.432931465426158
1101	12	0.4489339449149625
1101	13	0.4507337502000439
1101	14	0.8814726778185715
1101	15	0.7149360769720374
1101	16	0.7461798543471687
1101	17	0.7830953562391016
1101	18	0.7916522341914316
1101	19	0.5897600919180661
1101	20	0.6064499867702011
1101	21	0.7154174422518764
1101	22	0.7128155009745427
1101	23	0.7633298030911675
1101	24	0.7540195472354938
1101	25	0.8400055099873924
1102	1	0.35953884136723246
1102	2	0.3358688391323206
1102	3	0.34831324481456605
1102	4	0.48783424851870194
1102	5	0.29784429141053437
1102	6	0.20750537368895294
1102	7	0.23445199223011315
1102	8	0.35475418405528103
1102	9	0.3002121882025648
1102	10	0.6190735943915442
1102	11	0.43228564328801156
1102	12	0.3830650354532068
1102	13	0.3799713758665675
1102	14	0.7157655937362577
1102	15	0.8093187787318143
1102	16	0.7349843364090631
1102	17	0.6625101844987569
1102	18	0.7125580354224041
1102	19	0.7156473290182461
1102	20	0.6628386252179439
1102	21	0.7221927405291879
1102	22	0.6163158798832598
1102	23	0.779928234240316
1102	24	0.7080417825197785
1102	25	0.8289779328924425
1103	1	0.25529961130306944
1103	2	0.23361256882151338
1103	3	0.2649297767702343
1103	4	0.3151378065555335
1103	5	0.11245099085224552
1103	6	0.11763702938431209
1103	7	0.1691720155615073
1103	8	0.3431024467000583
1103	9	0.35794737693683487
1103	10	0.3928791105985283
1103	11	0.32219002625609505
1103	12	0.41616415219348113
1103	13	0.3511610683921944
1103	14	0.6139801175259478
1103	15	0.6267614918390549
1103	16	0.4757049013619863
1103	17	0.4199020685084207
1103	18	0.5293805918760341
1103	19	0.5086472873550463
1103	20	0.4621507658728916
1103	21	0.5042709006825135
1103	22	0.5785111136137786
1103	23	0.7315347670292525
1103	24	0.6653938781784774
1103	25	0.7211556680811739
1104	1	0.16756111523124062
1104	2	0.19270628374856358
1104	3	0.2314112582517147
1104	4	0.19906597715036867
1104	5	0.14461148467940887
1104	6	0.14761458269519778
1104	7	0.22935720074669064
1104	8	0.2738628282937725
1104	9	0.20247038479317325
1104	10	0.4501855079049264
1104	11	0.19529047518987852
1104	12	0.28137458990391906
1104	13	0.3280157260801815
1104	14	0.6102792196583806
1104	15	0.538456216867111
1104	16	0.3389905354809528
1104	17	0.4513723266453482
1104	18	0.49160000826211814
1104	19	0.43550755671531505
1104	20	0.4496984650872591
1104	21	0.40531467172628055
1104	22	0.5093135827495843
1104	23	0.4763944752222926
1104	24	0.5341845628024942
1104	25	0.5684901237489655
1105	1	0.37710716525990406
1105	2	0.3130501096979046
1105	3	0.28857022804557886
1105	4	0.4657231564574289
1105	5	0.3454227043905078
1105	6	0.26101668929275235
1105	7	0.3111438926053225
1105	8	0.5024704977137944
1105	9	0.3433209871395775
1105	10	0.43481174630814223
1105	11	0.4651135056544007
1105	12	0.5010546034688232
1105	13	0.4518870330749891
1105	14	0.7258081096776792
1105	15	0.6161409882419306
1105	16	0.45832017278121384
1105	17	0.5725573987896482
1105	18	0.4369386363495451
1105	19	0.47129771246950014
1105	20	0.507998542197285
1105	21	0.5521543212188078
1105	22	0.5253096747648556
1105	23	0.6221405551260446
1105	24	0.5728832503171262
1105	25	0.735458316871231
1106	1	0.13604335209835838
1106	2	-0.07715002768850354
1106	3	-0.10400426169988464
1106	4	0.03214409923192818
1106	5	-0.25259854723852726
1106	6	-0.24416642489337193
1106	7	-0.5507492144112689
1106	8	-0.008391394510276484
1106	9	0.16908184384449498
1106	10	-0.21804961703123382
1106	11	-0.3276826697763937
1106	12	-0.6166518204936295
1106	13	-0.09911038695658192
1106	14	-0.22030414632017425
1106	15	-0.06595328479325246
1106	16	0.10901313093309994
1106	17	-0.07812176332984677
1106	18	-0.39306830802132
1106	19	-0.5056472411405082
1106	20	0.09954912352898049
1106	21	0.07932378594158251
1106	22	0.34567606206739043
1106	23	-0.3196595026186273
1106	24	0.09685380141674926
1106	25	0.23820725910183427
1107	1	-0.9570928155471883
1107	2	-0.91424731226126
1107	3	-0.7471637923112873
1107	4	-0.7834023280086981
1107	5	-0.8096300606263966
1107	6	-0.8117112729158009
1107	7	-0.834262501728432
1107	8	-0.6960921237896898
1107	9	-0.7883450655604278
1107	10	-0.8189680181763137
1107	11	-0.7792717950551236
1107	12	-0.82808518280887
1107	13	-0.7142469272266467
1107	14	-0.9709044858225155
1107	15	-0.9044863566979515
1107	16	-0.8847062236575738
1107	17	-1.0778267689192091
1107	18	-1.2610180155406496
1107	19	-1.096166904477215
1107	20	-0.9557760061502663
1107	21	-1.0191244990984316
1107	22	-1.0046730516185818
1107	23	-0.9115070104703591
1107	24	-0.6610585549305839
1107	25	-0.785400426426726
1108	1	0.008880146789682186
1108	2	-0.1723785257193029
1108	3	-0.03979320742788772
1108	4	-0.12079543147715555
1108	5	-0.4485305414276297
1108	6	-0.0010574805256666764
1108	7	-0.045446653062690245
1108	8	0.09443913487376676
1108	9	-0.2669720682588519
1108	10	-0.5390074558736728
1108	11	-0.18235455899290542
1108	12	0.021514478662588488
1108	13	0.1052644373988727
1108	14	-0.24898852984878062
1108	15	-0.0700680874214683
1108	16	-0.8077016782462536
1108	17	-0.7433587590617989
1108	18	-0.6355756535360157
1108	19	-0.11235984087583972
1108	20	-0.9986047493301413
1108	21	-0.251592407280242
1108	22	-0.33231124082735874
1108	23	-0.2098394940687669
1108	24	-0.35999527686537647
1108	25	-1.150085107717615
1109	1	0.012178805933942272
1109	2	0.06343486900455275
1109	3	0.13577099092520584
1109	4	0.07971300484717148
1109	5	0.02463906284736339
1109	6	0.11192623011646674
1109	7	0.19093452318810478
1109	8	0.22292550954325918
1109	9	0.18699252641822284
1109	10	0.21306062371628087
1109	11	0.19933233864169608
1109	12	0.21025201064375665
1109	13	0.19544721848370727
1109	14	0.3563214842129483
1109	15	0.29709706230885047
1109	16	0.14212497457736362
1109	17	0.2594749788195827
1109	18	0.31341430145504745
1109	19	0.37127236362106686
1109	20	0.3473864278619061
1109	21	0.33317648335487604
1109	22	0.25820071092921637
1109	23	0.35153858033004326
1109	24	0.3742720050851247
1109	25	0.23711051859978483
1110	1	0.011393100451652316
1110	2	-0.04252227713386603
1110	3	0.04294465004387149
1110	4	-1.0276425850061628
1110	5	-0.3439467933335223
1110	6	-0.08861353974605635
1110	7	-0.013109058956629838
1110	8	0.11364312055042092
1110	9	0.17052212272526646
1110	10	0.006168684130173574
1110	11	0.05144218895149777
1110	12	0.10408303214491355
1110	13	0.08823763539781027
1110	14	0.347429426981094
1110	15	0.2968449642931432
1110	16	0.13781409850874837
1110	17	0.2374290073458738
1110	18	0.3986798026819187
1110	19	0.1794383785676743
1110	20	0.10390141088731042
1110	21	0.16166124823145367
1110	22	-0.4888010733645203
1110	23	0.02919450295276249
1110	24	0.21342961956563755
1110	25	0.3374707386533373
1111	1	-0.14872093237818973
1111	2	-0.372150650631788
1111	3	-0.24848935960283214
1111	4	-0.14451961010956182
1111	5	-0.11619612567513826
1111	6	-0.056048759821294425
1111	7	-0.13149249423872078
1111	8	0.010937012528508205
1111	9	-0.0880838630412839
1111	10	-0.08916092033961576
1111	11	-0.22844632794790698
1111	12	-0.11873091279548476
1111	13	-0.22893339669864332
1111	14	-0.22171292516492258
1111	15	-0.15736799560647796
1111	16	-0.19420468946221092
1111	17	-0.07272446660825693
1111	18	-0.0556179461080859
1111	19	-0.12023427049361857
1111	20	-0.16299600165714878
1111	21	-0.1195708997664724
1111	22	-0.13144842155949021
1111	23	-0.0839884460100464
1111	24	0.0392993942229225
1111	25	0.03475383065733695
1112	1	-0.07027842337900836
1112	2	-0.48446584120776137
1112	3	-0.25067453221557656
1112	4	-0.22518349064438326
1112	5	-0.16404814584920047
1112	6	-0.17205893938680106
1112	7	-0.06642028231402719
1112	8	-0.0017491034838599262
1112	9	-0.16982681418996037
1112	10	0.17369586609736132
1112	11	-0.006114342785267013
1112	12	0.03599956244796232
1112	13	-0.029718557762002717
1112	14	-0.29429797721168427
1112	15	-0.1873838425953025
1112	16	-0.14646216567502535
1112	17	-0.17096273143152274
1112	18	-0.189123086526493
1112	19	0.0022556876253005953
1112	20	-0.8355141992198227
1112	21	-1.1223051821941468
1112	22	-1.561129679119496
1112	23	-0.5427562021172108
1112	24	-0.34487806086514183
1112	25	-1.4052443769604892
1113	1	-0.23401490482596188
1113	2	-0.14039036372980718
1113	3	-0.1486222760156899
1113	4	-0.481493425116021
1113	5	-0.2773987158926232
1113	6	-0.1688988943693559
1113	7	-0.21979426009831826
1113	8	-0.08684256796269807
1113	9	-0.10724323889894549
1113	10	-0.07956223837508247
1113	11	-0.16145733444771793
1113	12	-0.03533144897954088
1113	13	-0.09023555917379156
1113	14	-0.4749055129725903
1113	15	-0.39839979848353124
1113	16	-0.33312940948845693
1113	17	-0.34902388457075517
1113	18	-0.8257513729333539
1113	19	-1.072709017630288
1113	20	-1.1516542194643549
1113	21	-0.9354138500506295
1113	22	-0.4625775960636389
1113	23	-1.7247482241730978
1113	24	-0.8642468842848672
1113	25	-0.4795408128572447
1114	1	-0.3042451171314333
1114	2	-0.48458159324644967
1114	3	-0.48727977884986373
1114	4	-0.6735777031743595
1114	5	-0.6895450881486269
1114	6	-0.45853418757764075
1114	7	-0.5436438427016341
1114	8	-0.2804315352923865
1114	9	-1.172684339589452
1114	10	-0.45412422951184084
1114	11	-0.44681302340988793
1114	12	-0.4556061063020209
1114	13	-0.43903425545329355
1114	14	-0.8383884563921526
1114	15	-1.3131426615155775
1114	16	-1.2489647181558752
1114	17	-1.6587444606812412
1114	18	-1.8223838577318645
1114	19	-1.864287653103851
1114	20	-1.0587241978215616
1114	21	-0.810595494902735
1114	22	-0.6737732314341471
1114	23	-0.39501257540467094
1114	24	-0.9507214282403496
1114	25	-0.6578052671497261
1115	1	-0.7826658790459255
1115	2	-0.461696236722716
1115	3	-0.6150040221272981
1115	4	-0.7656005971737088
1115	5	-0.42106263044554737
1115	6	-0.4029820882888089
1115	7	-0.39294668558527235
1115	8	-0.25656136336392166
1115	9	-0.5298735699073163
1115	10	-0.23522241548310774
1115	11	-0.5063359578981732
1115	12	-0.5236872719721362
1115	13	-0.41034130669421387
1115	14	-0.4628641636559294
1115	15	-1.4341704637078365
1115	16	-0.7973079577812143
1115	17	-0.6491214873858695
1115	18	-2.264794464399337
1115	19	-1.091918257939973
1115	20	-0.88684256970555
1115	21	-0.9417655409417662
1115	22	-0.7443286212273179
1115	23	-0.3840002477707287
1115	24	-0.4789009072661443
1115	25	-0.5992013924835291
1116	1	-1.334474631409826
1116	2	-0.5559460917025696
1116	3	-1.5870036650974804
1116	4	-0.8709163336270151
1116	5	-0.7435684241012552
1116	6	-0.37367057899130884
1116	7	-1.1469604945269936
1116	8	-2.800639781256777
1116	9	-1.3291890473171364
1116	10	-0.6584148155172684
1116	11	-0.4053201055439004
1116	12	-0.5998419701848121
1116	13	-1.5259913329310066
1116	14	-1.4866308646524757
1116	15	-1.3427793730876019
1116	16	-0.9696372850284973
1116	17	-1.4521677629746321
1116	18	-2.1494684096653884
1116	19	-1.1120667447549621
1116	20	-0.7021966422914332
1116	21	-0.6286605667831537
1116	22	-0.40233190410388175
1116	23	-0.22017845973255845
1116	24	-0.4157275042493467
1116	25	-0.3401066502825003
1201	1	0.43355350681177995
1201	2	0.3468679225420388
1201	3	0.4589915216248315
1201	4	0.5597865858239217
1201	5	0.40371046922904696
1201	6	0.2668545945872083
1201	7	0.15379204722553652
1201	8	0.42372760293704204
1201	9	0.3648318032346438
1201	10	0.49258413517981126
1201	11	0.3816440300891131
1201	12	0.41881205653478304
1201	13	0.377450752585176
1201	14	0.8360680685300252
1201	15	0.7300337191534396
1201	16	0.6117029431918297
1201	17	0.6748841533346877
1201	18	0.6289191719943545
1201	19	0.5751798679733326
1201	20	0.5542226765469991
1201	21	0.5611019980910612
1201	22	0.631210472000582
1201	23	0.6823113689790858
1201	24	0.7094166679459946
1201	25	0.8496012142856557
1202	1	0.3017281827316438
1202	2	0.3108730025625306
1202	3	0.3573395191314326
1202	4	0.39432244069161726
1202	5	0.19391045371947513
1202	6	0.018403221121374713
1202	7	0.0708983636470748
1202	8	0.07751687531352047
1202	9	0.2371097356137568
1202	10	0.42322753240325994
1202	11	0.3116069766196503
1202	12	0.18463838831334556
1202	13	0.19015709663579372
1202	14	0.5861907467025915
1202	15	0.33830102027178555
1202	16	0.37575796318689214
1202	17	0.37849156962219155
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2210	23	0.6389043261154583
2210	24	0.6232059962568783
2210	25	0.7859131184901216
2211	1	0.13534692991505892
2211	2	-0.09165007337330522
2211	3	0.05265418170284164
2211	4	0.10949589851614405
2211	5	0.11605533078150643
2211	6	0.09576627133058935
2211	7	0.15054807903536016
2211	8	0.21112964295281245
2211	9	0.23912221681829493
2211	10	0.36512459382193185
2211	11	0.12475263977297146
2211	12	0.12493745302514014
2211	13	0.0011756463484076107
2211	14	-0.025224786402937127
2211	15	0.11605697028279849
2211	16	0.11337935887083006
2211	17	0.06364932020520087
2211	18	0.10641553117437752
2211	19	0.08858300095795357
2211	20	0.0867234644676742
2211	21	0.12783748131533912
2211	22	0.09002005157382342
2211	23	0.25023947977182104
2211	24	0.25196949236450195
2211	25	0.22457073356248713
2212	1	0.09922391409191533
2212	2	0.06147475643469846
2212	3	0.09699605710344628
2212	4	-0.03188138938796925
2212	5	0.045303218306067095
2212	6	0.09462362844262628
2212	7	0.11610247553977615
2212	8	0.17199867994888507
2212	9	0.07579236063838835
2212	10	0.20798595594441593
2212	11	0.12000150760343331
2212	12	0.14451529308445998
2212	13	0.03471865622268666
2212	14	0.11560181256397328
2212	15	-0.034513408559355696
2212	16	-0.04879256332472437
2212	17	0.10394408227154628
2212	18	0.15758142490754068
2212	19	0.0995953134325589
2212	20	0.03609317804239254
2212	21	0.06266640955610825
2212	22	-0.44640920511132265
2212	23	8.285011835411638E-4
2212	24	0.004063744169059191
2212	25	0.04950590350312467
2213	1	-0.034092080091589594
2213	2	-0.7185160616973256
2213	3	-0.17115645648216926
2213	4	-0.05510048136822121
2213	5	0.06299545627801706
2213	6	0.029894020662077025
2213	7	0.0729823743421889
2213	8	0.16751414528831532
2213	9	-0.18911044160466162
2213	10	-1.6291279031592296
2213	11	-0.4976501409950487
2213	12	-0.13103236790363582
2213	13	-0.036820902653580845
2213	14	-0.06205373734321995
2213	15	-0.12089429451109623
2213	16	-0.11136797877469462
2213	17	-0.11900270624204978
2213	18	0.13470497817223048
2213	19	0.03347936180878719
2213	20	-0.03930099206255022
2213	21	-0.09078979529806475
2213	22	0.1709394024367299
2213	23	-0.8467972175045162
2213	24	-1.5492704955289227
2213	25	-1.4047390356325238
2214	1	-0.10199368042859902
2214	2	-0.23126085338810043
2214	3	-0.13282444248944358
2214	4	-0.18622299333100756
2214	5	-0.09547625351025602
2214	6	-0.26342452216866263
2214	7	-0.1453862031308759
2214	8	-0.04408270434234396
2214	9	-0.3512080620079985
2214	10	-0.051056501371405716
2214	11	-0.10182677987069155
2214	12	-0.2851509094998974
2214	13	-0.13064966767361597
2214	14	-0.1121186627889017
2214	15	-0.19181105106266472
2214	16	-0.2581998639195362
2214	17	-0.19209764784387318
2214	18	-0.045812643126097896
2214	19	-0.7855191429385258
2214	20	-0.4522826478272961
2214	21	-0.4405199091317016
2214	22	-0.6367852982426925
2214	23	-1.8982844075559129
2214	24	-1.4021224315648269
2214	25	-1.7030430271393655
2215	1	-0.41843096846199046
2215	2	-0.28212522786455807
2215	3	-1.2419300875034232
2215	4	-1.0096711775637957
2215	5	-0.405419242362818
2215	6	-0.36509437714019555
2215	7	-0.24573807434858708
2215	8	-0.2215090624804148
2215	9	-0.3632028521444326
2215	10	-1.357999665797493
2215	11	-0.5275820120504235
2215	12	-0.6242851398937432
2215	13	-0.5616391486321604
2215	14	-0.8968953001598194
2215	15	-1.2197030780737919
2215	16	-0.738270347527056
2215	17	-0.6144718334516881
2215	18	-0.5196027523659339
2215	19	-1.116356415838311
2215	20	-1.353130676822346
2215	21	-0.8433859252501748
2215	22	-0.494772327083453
2215	23	-0.6395655120020649
2215	24	-1.771300203601811
2215	25	-0.82438413694139
2216	1	-0.5348149211790911
2216	2	-0.46704944940809767
2216	3	-1.5338035383562456
2216	4	-0.8975054870641088
2216	5	-0.6906021276402257
2216	6	-0.5230132966321435
2216	7	-0.39887239777956296
2216	8	-0.562671202294581
2216	9	-0.3413183179390771
2216	10	-0.34381293186895334
2216	11	-0.42440651557983244
2216	12	-0.3504334137779139
2216	13	-1.0982813751950147
2216	14	-0.753460653306675
2216	15	-1.2299564953933952
2216	16	-1.046016584999947
2216	17	-1.8051100504278423
2216	18	-0.9694395187305958
2216	19	-0.6103727003553274
2216	20	-0.5947633432999524
2216	21	-0.4469020757965083
2216	22	-0.5332857320490092
2216	23	-0.4917571457144092
2216	24	-0.6623114609810514
2216	25	-0.5724494594236873
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3913.0
3	377.0
4	465.0
5	430.0
6	305.0
7	233.0
8	369.0
9	728.0
10	1448.0
11	2541.0
12	4557.0
13	6806.0
14	9318.0
15	12110.0
16	14273.0
17	15152.0
18	15670.0
19	16114.0
20	16742.0
21	17629.0
22	19057.0
23	20014.0
24	20984.0
25	22211.0
26	23215.0
27	26243.0
28	29641.0
29	35080.0
30	42743.0
31	54023.0
32	72277.0
33	103104.0
34	150122.0
35	240203.0
36	441736.0
37	1558262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.751874894398654	21.262264393986285	13.101325432819142	22.88453527879592
2	14.954004283800085	21.39607774996909	40.49918634530369	23.150731620927132
3	18.268578103146048	26.59278482302948	30.364435116378942	24.77420195744553
4	11.818026835676608	17.873703844842456	38.86138911731082	31.446880202170117
5	12.159696087006528	39.597827511588655	35.37412031873149	12.868356082673321
6	30.502619403773174	39.023635457062234	17.52136495065308	12.952380188511517
7	25.4655252961978	33.60504152817814	23.447096101958746	17.482337073665313
8	23.50689882118885	38.296705298550044	21.288025074346294	16.908370805914814
9	23.711616915381313	17.368566190295535	22.33996219559684	36.579854698726315
10	14.243274142219132	31.172311662610646	34.27603428449175	20.30837991067847
11	31.598209866455186	26.417871390715597	23.039129677736526	18.944789065092696
12	19.93964630690411	28.841933016023575	32.024555911724114	19.1938647653482
13	26.185073058321308	24.561822217899078	26.71688642826319	22.536218295516424
14	19.532594487298137	24.506987552987788	28.651022756631683	27.309395203082392
15	22.86207945244851	31.26009698452325	24.147709233581644	21.7301143294466
16	20.669284628110223	32.16429657604064	26.69297996423976	20.47343883160938
17	19.5847212664835	31.273638867453236	28.95688364154445	20.184756224518818
18	19.714045556860853	30.664336073340166	29.910447062834113	19.711171306964875
19	20.326700420006155	29.855061613937238	30.147197669595826	19.671040296460777
20	21.577536863233817	29.395840999074597	29.18402424429577	19.842597893395816
21	21.438341903804194	29.852542450482932	28.72442853223209	19.98468711348079
22	20.346784761442606	29.67853128813073	29.3935878707548	20.581096079671866
23	20.00679800792299	30.128844642792092	29.27929418362576	20.585063165659164
24	20.324527378550428	29.65130504891677	29.274083671278227	20.750083901254573
25	20.94452990098851	29.189671412345987	28.28509105300066	21.580707633664844
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7431.0
1	7431.0
2	6446.5
3	5462.0
4	5462.0
5	5462.0
6	7384.5
7	9307.0
8	9307.0
9	9307.0
10	13570.0
11	17833.0
12	17833.0
13	17833.0
14	27239.0
15	36645.0
16	36645.0
17	36645.0
18	55209.5
19	73774.0
20	73774.0
21	73774.0
22	101136.0
23	128498.0
24	128498.0
25	128498.0
26	162078.5
27	195659.0
28	195659.0
29	195659.0
30	229185.0
31	262711.0
32	262711.0
33	262711.0
34	289095.5
35	315480.0
36	315480.0
37	315480.0
38	330151.0
39	344822.0
40	344822.0
41	344822.0
42	341721.5
43	338621.0
44	338621.0
45	338621.0
46	353990.0
47	369359.0
48	369359.0
49	369359.0
50	337890.0
51	306421.0
52	306421.0
53	306421.0
54	268445.5
55	230470.0
56	230470.0
57	230470.0
58	197232.5
59	163995.0
60	163995.0
61	163995.0
62	133820.5
63	103646.0
64	103646.0
65	103646.0
66	78130.5
67	52615.0
68	52615.0
69	52615.0
70	37590.0
71	22565.0
72	22565.0
73	22565.0
74	15578.0
75	8591.0
76	8591.0
77	8591.0
78	5718.0
79	2845.0
80	2845.0
81	2845.0
82	1892.0
83	939.0
84	939.0
85	939.0
86	610.0
87	281.0
88	281.0
89	281.0
90	180.0
91	79.0
92	79.0
93	79.0
94	56.5
95	34.0
96	34.0
97	34.0
98	23.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30886279454120036
2	0.18094823546285224
3	0.19779226475478595
4	0.2186388356606445
5	0.24145332286001608
6	0.19382307765431048
7	0.1890867367444994
8	0.21186786943042166
9	0.21280179580700412
10	0.2219742870055819
11	0.19732530156649472
12	0.20232847858390077
13	0.21493648466776402
14	0.20452987647155943
15	0.19562422138057664
16	0.19829258245652653
17	0.1979923918354822
18	0.20046062583073584
19	0.19999366264244461
20	0.19632466616301353
21	0.19492377659813984
22	0.19752542864719097
23	0.2015279702611158
24	0.21623731069228957
25	0.21083387951349106
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2998095.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.94102766426218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.58060718933865	70.71585190314987
2	8.009050216808959	12.644853094591618
3	1.3181955525308884	3.1217913473774463
4	0.39845094488245	1.2581650825126756
5	0.17320083841203834	0.6836326088279061
6	0.10946306135756659	0.5184675932905495
7	0.07172657326133136	0.3963518582859841
8	0.051469107901781656	0.32504194165835526
9	0.0423697557218342	0.30102408527088104
>10	0.21685102855441532	3.0999231736788544
>50	0.016138453322809013	0.8984686318577538
>100	0.010096568335354626	1.4736193304595224
>500	7.227154059603178E-4	0.42416407309829923
>1k	0.0014454308113054602	2.5398497670991014
>5k	1.2753801276224648E-4	0.6282756238667866
>10k+	8.502534184149766E-5	0.9705198849742855
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	15549	0.5186293296243114	No Hit
CCCATGTACTCTGCGTTGATACCAC	13370	0.4459498448181262	No Hit
GAGTACATGGGAAGCAGTGGTATCA	7908	0.26376749235764707	No Hit
CATGTACTCTGCGTTGATACCACTG	5693	0.18988724506728438	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5120	0.17077510886079325	No Hit
GCGTTGATACCACTGCTTCCCATGT	4504	0.15022872857597908	No Hit
GCTTCCCATGTACTCTGCGTTGATA	4458	0.1486944209573079	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4455	0.14859435741695978	No Hit
GTATCAACGCAGAGTACATGGGAAG	4117	0.13732053187107146	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	4012	0.13381830795888724	No Hit
GGTATCAACGCAGAGTACATGGGAA	3929	0.1310498833425892	No Hit
TATCAACGCAGAGTACATGGGAAGC	3564	0.11887548593356781	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3562	0.11880877690666908	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3458	0.11533990750793421	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3071	0.10243171080302658	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.670902689874738E-5	0.0
8	0.0	0.0	0.0	6.670902689874738E-5	0.0
9	0.0	0.0	0.0	6.670902689874738E-5	0.0
10	0.0	0.0	0.0	6.670902689874738E-5	0.0
11	0.0	3.335451344937369E-5	0.0	6.670902689874738E-5	0.0
12	0.0	3.335451344937369E-5	0.0	6.670902689874738E-5	0.0
13	0.0	3.335451344937369E-5	0.0	1.3341805379749475E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACTG	380	0.0	10.255632	5
GCCTTAG	240	0.0	9.908233	1
TAGACAG	420	0.0	9.731534	5
GACTGTC	205	4.1836756E-11	9.730055	7
GTCTAAC	170	1.6327249E-8	9.511904	1
TAGGCGT	80	0.0044946047	9.505856	4
ATTGGAC	140	1.0517433E-6	9.505061	3
CTAGGGC	195	1.6680133E-9	9.261341	3
TAGGGCC	175	2.6455382E-8	9.23426	4
ACAGTCC	300	0.0	9.182081	8
AGGCGTG	435	0.0	9.1708555	7
TCAGTAC	230	4.1836756E-11	9.091798	3
TAACCCG	95	0.0018153869	9.004946	5
CCAGTAC	385	0.0	8.88785	3
AGTACTC	290	0.0	8.849688	5
TATACTG	795	0.0	8.847627	5
GCTAGAC	195	1.6354534E-8	8.773902	3
TAGGACA	360	0.0	8.713701	4
CCTATAC	285	0.0	8.671283	3
CTAGACT	310	0.0	8.585935	4
>>END_MODULE