FastQCFastQC Report
Wed 25 May 2016
SRR1295059_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295059_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998095
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC153790.5129590623379179No Hit
CCCATGTACTCTGCGTTGATACCAC148220.4943805983466168No Hit
GTATCAACGCAGAGTACTTTTTTTT136700.45595619885293825No Hit
GGTATCAACGCAGAGTACTTTTTTT131230.4377112799961309No Hit
TATCAACGCAGAGTACTTTTTTTTT97530.32530656967174154No Hit
GAGTACATGGGAAGCAGTGGTATCA83710.2792106320847071No Hit
CATGTACTCTGCGTTGATACCACTG63790.2127684412935547No Hit
ACGCAGAGTACTTTTTTTTTTTTTT58560.1953240307595323No Hit
GCTTCCCATGTACTCTGCGTTGATA51330.17120871753563513No Hit
ACGCAGAGTACATGGGAAGCAGTGG50070.16700604884101403No Hit
GCGTTGATACCACTGCTTCCCATGT45630.15219664486949214No Hit
GTATCAACGCAGAGTACATGGGAAG44150.14726017687898482No Hit
GGTATCAACGCAGAGTACATGGGAA43900.14642631404275047No Hit
TATCAACGCAGAGTACATGGGAAGC39280.13101652882913983No Hit
ACTCTGCGTTGATACCACTGCTTCC35130.11717440574764974No Hit
ATTCCATTCCATTCCATTCCATTCC30530.10183132956093786No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAC551.96162E-413.8177573
ACGCCGA550.0030706812.0903357
ACACCGT550.0030706812.0903356
AACGCCG600.00587989211.0828086
GCGTCAG1303.7880818E-710.24189951
CCCGTAC750.002649473510.1330223
ACCGTTC856.599628E-410.0583478
GGAACGA951.6463209E-49.99919215
GTATAGG3400.09.5103351
GTCTTAG3150.09.3593781
TAGGACT3800.09.2497164
GTATTAC3150.09.0574631
CTAGGAC2950.09.0166723
CCGGGTT1901.0602889E-88.99927217
TTAGTAC1804.224603E-88.9719473
TTATACT5900.08.855664
TACACAG7450.08.7983885
CGGGTTC1951.6516424E-88.76881518
TCTAGAC2502.5465852E-118.7397323
GTGTAAG4800.08.7178081
Produced by FastQC (version 0.11.5)