FastQCFastQC Report
Wed 25 May 2016
SRR1295058_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295058_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669333
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1556069.321447548212369No Hit
CCCATGTACTCTGCGTTGATACCAC1458518.737082415551601No Hit
GAGTACATGGGAAGCAGTGGTATCA826834.95305610084986No Hit
CATGTACTCTGCGTTGATACCACTG692704.149561531461967No Hit
GCGTTGATACCACTGCTTCCCATGT514473.0818895930290724No Hit
ACGCAGAGTACATGGGAAGCAGTGG382392.290675377531026No Hit
TATCAACGCAGAGTACATGGGAAGC353092.1151561731541877No Hit
ACTCTGCGTTGATACCACTGCTTCC326981.9587463975132582No Hit
GTATCAACGCAGAGTACATGGGAAG326221.9541936809492173No Hit
GCTTCCCATGTACTCTGCGTTGATA317551.902256769619962No Hit
GGTATCAACGCAGAGTACATGGGAA299141.791973201272604No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA206601.2376200554353145No Hit
GCAGTGGTATCAACGCAGAGTACAT180891.083606446407038No Hit
CAGTGGTATCAACGCAGAGTACATG170831.0233428560988131No Hit
GCAGAGTACATGGGAAGCAGTGGTA163720.9807509945589047No Hit
GTTGATACCACTGCTTCCCATGTAC158760.9510385285620065No Hit
GTACTCTGCGTTGATACCACTGCTT154550.925818874963833No Hit
GTGGTATCAACGCAGAGTACATGGG151180.9056311712522306No Hit
ATACCACTGCTTCCCATGTACTCTG150060.8989219047368021No Hit
GGGAAGCAGTGGTATCAACGCAGAG139650.8365616686425057No Hit
AAGCAGTGGTATCAACGCAGAGTAC135860.8138579899876178No Hit
ACATGGGAAGCAGTGGTATCAACGC135410.8111623025483831No Hit
CATGGGAAGCAGTGGTATCAACGCA129360.7749202825320053No Hit
CTGCTTCCCATGTACTCTGCGTTGA127970.7665936035530359No Hit
TACCACTGCTTCCCATGTACTCTGC126330.7567693204411583No Hit
CCACTGCTTCCCATGTACTCTGCGT122730.7352038209272805No Hit
GATACCACTGCTTCCCATGTACTCT111630.6687101974261576No Hit
GTACATGGTAAGCAGTGGTATCAAC104720.6273164191925756No Hit
CTTCCCATGTACTCTGCGTTGATAC101580.6085065112832491No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA94190.5642373331144834No Hit
ATGGGAAGCAGTGGTATCAACGCAG92250.5526159250431161No Hit
CCATGTACTCTGCGTTGATACCACT90890.5444689585600956No Hit
TTCCCATGTACTCTGCGTTGATACC85270.5108028176523198No Hit
ACCATGTACTCTGCGTTGATACCAC81230.48660153486452373No Hit
TGATACCACTGCTTCCCATGTACTC74190.4444290024818296No Hit
GTACATGGGTAAGCAGTGGTATCAA52920.31701284285400216No Hit
ATCAACGCAGAGTACATGGGAAGCA50570.30293536400466536No Hit
CCCCATGTACTCTGCGTTGATACCA49090.2940695475378489No Hit
GGTAAGCAGTGGTATCAACGCAGAG46530.27873408121686927No Hit
GAACAAAAAAAAAAAAAAAAAAAAA45420.27208471886675695No Hit
GAGTACATGGTAAGCAGTGGTATCA43530.26076283162197117No Hit
TCCATGTACTCTGCGTTGATACCAC42950.2572883900336242No Hit
GGAAGCAGTGGTATCAACGCAGAGT41950.2512979735019915No Hit
GCTTACCATGTACTCTGCGTTGATA37140.22248406998483825No Hit
GTACATGGAAAGCAGTGGTATCAAC34100.20427320372867486No Hit
AAACAAAAAAAAAAAAAAAAAAAAA33300.1994808705033687No Hit
AAAAAGTACTCTGCGTTGATACCAC31910.19115419152439928No Hit
CTGCGTTGATACCACTGCTTCCCAT30810.1845647333396033No Hit
GCGTTGATACCACTGCTTACCATGT29370.17593853353405223No Hit
CTCTGCGTTGATACCACTGCTTCCC28050.16803118371229706No Hit
GAGTACATGGAAAGCAGTGGTATCA27520.16485626295053174No Hit
TGGGAAGCAGTGGTATCAACGCAGA25850.15485226734270513No Hit
ACTCTGCGTTGATACCACTGCTTAC25250.15125801742372552No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24040.14400961342044996No Hit
GTACATGGAAGCAGTGGTATCAACG23990.14371009259386833No Hit
AAAGTACTCTGCGTTGATACCACTG23590.14131392598121525No Hit
AGTGGTATCAACGCAGAGTACATGG23300.13957670518704177No Hit
GCTTTCCATGTACTCTGCGTTGATA22860.13694092191312338No Hit
TCCCATGTACTCTGCGTTGATACCA22390.134125426143256No Hit
ACTGCTTCCCATGTACTCTGCGTTG22160.1327476303409805No Hit
ACGCAGAGTACATGGTAAGCAGTGG21640.1296326137445315No Hit
AAAAACAAAAAAAAAAAAAAAAAAA21520.12891376376073557No Hit
TATCAACGCAGAGTACATGGTAAGC20690.12394171803948044No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA20610.12346248471694983No Hit
CTGCTTACCATGTACTCTGCGTTGA20500.12280353889847022No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN20140.12064698894708246No Hit
ATGGTAAGCAGTGGTATCAACGCAG20010.11986823479797021No Hit
TCAACGCAGAGTACATGGGAAGCAG19790.11855034316101101No Hit
AACGCAGAGTACATGGGAAGCAGTG19330.11579475155645998No Hit
GTACAAAAAAAAAAAAAAAAAAAAA19090.11435705158886814No Hit
GTATCAACGCAGAGTACTTTTTTTT18970.1136382016050722No Hit
GAAAACAAAAAAAAAAAAAAAAAAA18900.11321887244785793No Hit
TTGATACCACTGCTTCCCATGTACT18450.1105231850086232No Hit
GTATCAACGCAGAGTACATGGTAAG18210.10908548504103135No Hit
GGTATCAACGCAGAGTACATGGTAA18050.10812701839597012No Hit
GCGTTGATACCACTGCTTTCCATGT17150.1027356435175007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA8350.016.286371
GTACAAG1900.016.0166281
GTATCTA551.1311124E-515.54152518
TACATAA5700.015.5077192
GTACATA6800.015.2437671
TACAAAA8800.015.229172
AATAAGC508.7288776E-515.197076
AGAACAA9650.015.0696772
TACAAGA1650.014.9771512
CGGCGTT456.7664176E-414.7767049
GGTGTCC456.7664176E-414.7767048
CTCTCTT653.381334E-614.611690519
GTAAATA851.6934791E-814.5445121
GTAAAAA4900.014.361851
GTCTCAC400.005243058314.2648091
GTATATA602.5434425E-514.2648091
AAGCATA602.5702391E-514.2489659
TATCTAC602.5746082E-514.24639919
GTCGAAA551.9644934E-413.81468915
TCGAAAC500.001502485613.296638516
Produced by FastQC (version 0.11.5)