##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295058_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1669333 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.904499581569404 33.0 33.0 33.0 15.0 33.0 2 29.932277143026585 33.0 33.0 33.0 15.0 33.0 3 30.235619855355402 33.0 33.0 33.0 22.0 33.0 4 33.04970308500461 37.0 33.0 37.0 15.0 37.0 5 34.31665102169549 37.0 33.0 37.0 27.0 37.0 6 33.659834197251236 37.0 37.0 37.0 22.0 37.0 7 33.982194684943025 37.0 33.0 37.0 27.0 37.0 8 33.676144903383566 37.0 33.0 37.0 22.0 37.0 9 33.540080978450675 37.0 33.0 37.0 22.0 37.0 10 33.75274675574017 37.0 33.0 37.0 27.0 37.0 11 33.93012897965834 37.0 33.0 37.0 27.0 37.0 12 34.0124792357187 37.0 33.0 37.0 27.0 37.0 13 33.57635654479963 37.0 33.0 37.0 22.0 37.0 14 35.09270229486867 37.0 33.0 40.0 27.0 40.0 15 34.967672118145394 37.0 33.0 40.0 22.0 40.0 16 34.99683885719626 37.0 33.0 40.0 27.0 40.0 17 35.06605931830258 37.0 33.0 40.0 27.0 40.0 18 34.87125576502711 37.0 33.0 40.0 22.0 40.0 19 34.67647557437611 37.0 33.0 40.0 22.0 40.0 20 34.83954849032518 37.0 33.0 40.0 22.0 40.0 21 34.6878166309538 37.0 33.0 40.0 22.0 40.0 22 34.79795163697117 37.0 33.0 40.0 22.0 40.0 23 34.811265337712726 37.0 33.0 40.0 22.0 40.0 24 34.96181408981911 37.0 33.0 40.0 27.0 40.0 25 33.795069647577805 37.0 33.0 40.0 15.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4398219313829941 1101 2 0.41044492524293474 1101 3 0.5519204063169063 1101 4 0.4439944135693068 1101 5 0.3753265410900539 1101 6 0.21141573583782503 1101 7 0.08188372303808933 1101 8 0.2125727280298335 1101 9 0.22353875105879695 1101 10 0.49068341157622797 1101 11 0.3235205015221325 1101 12 0.36787210817919913 1101 13 0.40016111132784005 1101 14 0.9915887748836738 1101 15 0.6557772515005666 1101 16 0.6221262869646864 1101 17 0.6624446589449846 1101 18 0.747416374309779 1101 19 0.6169953721206554 1101 20 0.6973709390628997 1101 21 0.5913847677667903 1101 22 0.5357395033691077 1101 23 0.7338960346223118 1101 24 0.8728166926147409 1101 25 1.0543068235953328 1102 1 0.6171280171646814 1102 2 0.5492097311960862 1102 3 0.5090935789377831 1102 4 0.7555332423914436 1102 5 0.4667593364752278 1102 6 0.46183997568984125 1102 7 0.4035268522623596 1102 8 0.6854263290286298 1102 9 0.5650852547494551 1102 10 0.8864113038875701 1102 11 0.661917833355858 1102 12 0.8935790272323274 1102 13 0.674540490335481 1102 14 1.2307521788470197 1102 15 0.9758691377144544 1102 16 1.0579806554503293 1102 17 1.0577732458849738 1102 18 1.2959523135615996 1102 19 0.9679777199230628 1102 20 1.0865447766032617 1102 21 0.9634104025350041 1102 22 0.951899522609331 1102 23 1.0127699864015014 1102 24 0.9326017962962041 1102 25 1.13913958573454 1103 1 0.40019073900918656 1103 2 0.3795182179449199 1103 3 0.24186154295283657 1103 4 0.3956378995306551 1103 5 0.4002627290360863 1103 6 0.12815272599253547 1103 7 0.314456520871083 1103 8 0.3284606271807462 1103 9 0.2959615254506289 1103 10 0.47834815085840887 1103 11 0.22961520381936396 1103 12 0.4419513914005364 1103 13 0.39430082065742056 1103 14 0.5780762468845069 1103 15 0.5223501535165056 1103 16 0.6145365594030849 1103 17 0.5658201863711483 1103 18 0.8124473687359881 1103 19 0.6779684408293107 1103 20 0.5630634951914573 1103 21 0.6913639311777402 1103 22 0.759336940468657 1103 23 0.6639267685911605 1103 24 0.7412023778780608 1103 25 0.7165665516778503 1104 1 0.28534043353601746 1104 2 0.21044579165141286 1104 3 0.24285950646505938 1104 4 0.3018115840525013 1104 5 0.17610457590234319 1104 6 0.13303522656291733 1104 7 0.12979524540442355 1104 8 0.43063907759100317 1104 9 0.45306768476988424 1104 10 0.5693394387468018 1104 11 0.4727515639988482 1104 12 0.3156874592045895 1104 13 0.4509439113867586 1104 14 0.8258508981042922 1104 15 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9 -0.14239942886949564 2213 10 -1.2775794074119915 2213 11 -0.24203097262847706 2213 12 0.11599539597109754 2213 13 0.08199122504928624 2213 14 0.07745595598354527 2213 15 -0.012181664313850149 2213 16 -0.07207048373376779 2213 17 -0.1275688185001158 2213 18 0.0304409596360955 2213 19 -6.169178998689517E-4 2213 20 -0.08832270892033023 2213 21 0.04356802578498531 2213 22 0.07355122434194783 2213 23 -0.8432134497993502 2213 24 -1.4570759282638477 2213 25 -1.530437237079596 2214 1 0.09345180908085737 2214 2 -0.19932912379938372 2214 3 0.05206442727802596 2214 4 0.05849368045056025 2214 5 0.0046732701079577055 2214 6 -0.05938820767588027 2214 7 -0.03271965538132804 2214 8 -0.047851295129198945 2214 9 -0.2317898799033955 2214 10 -0.011430631045875828 2214 11 -0.18847430446890456 2214 12 0.0038495853831079785 2214 13 -0.07870453562414781 2214 14 -0.1145445515088852 2214 15 -0.09634098450293749 2214 16 -0.1840810742994634 2214 17 0.030316416665868928 2214 18 -0.05976963541308322 2214 19 -0.5931818199333208 2214 20 -0.3334787556878567 2214 21 -0.31818655297482934 2214 22 -0.5228434807567766 2214 23 -1.9669371468816976 2214 24 -1.3189521334987475 2214 25 -1.9209071111556497 2215 1 -0.7673211798799109 2215 2 -0.3870839097589496 2215 3 -1.3584708674039305 2215 4 -1.1476934212973866 2215 5 -0.4544711691866681 2215 6 -0.4505619076968017 2215 7 -0.38185781857610124 2215 8 -0.3548161478142262 2215 9 -0.3511803700517717 2215 10 -1.5738187621666597 2215 11 -0.5973787378457374 2215 12 -0.634081058512372 2215 13 -0.6990974680191115 2215 14 -0.8814696208869535 2215 15 -1.2375649048808484 2215 16 -0.916483086812363 2215 17 -0.7763383296490929 2215 18 -0.7366473508665052 2215 19 -1.3002451484391813 2215 20 -1.7005122409344011 2215 21 -0.9137873884011611 2215 22 -0.5403799208558482 2215 23 -0.8122257376718949 2215 24 -2.0188334308367786 2215 25 -1.2507621598329877 2216 1 -0.6231538176687046 2216 2 -0.6762618045017668 2216 3 -1.4650595195237806 2216 4 -1.0863299268816178 2216 5 -0.8374851198228797 2216 6 -0.796763098923293 2216 7 -0.7576721734798326 2216 8 -0.9190515314425269 2216 9 -0.7064701992033164 2216 10 -0.5748879134887659 2216 11 -0.7195430453388809 2216 12 -0.707842847282592 2216 13 -1.4077193813627815 2216 14 -1.1314261848401443 2216 15 -1.5705791394127857 2216 16 -1.3490623930847576 2216 17 -2.2654929449419825 2216 18 -1.4595690921693603 2216 19 -1.0790835606671365 2216 20 -0.8015081471579606 2216 21 -0.6562604608910974 2216 22 -0.8627730483080001 2216 23 -0.5604653002453546 2216 24 -0.7521758488334953 2216 25 -0.8107061755948806 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2222.0 3 225.0 4 252.0 5 235.0 6 158.0 7 144.0 8 167.0 9 296.0 10 631.0 11 1056.0 12 2095.0 13 4016.0 14 7762.0 15 14153.0 16 20002.0 17 21143.0 18 20196.0 19 19293.0 20 19048.0 21 19728.0 22 19469.0 23 18330.0 24 18706.0 25 18379.0 26 17842.0 27 18340.0 28 19222.0 29 20978.0 30 24750.0 31 31067.0 32 41270.0 33 57151.0 34 81546.0 35 129909.0 36 241469.0 37 758083.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.27947126992082 17.4695081281614 8.45113669275706 23.79988390916072 2 8.35366783132429 29.445273294452733 30.038024410999714 32.16303446322327 3 24.76896869642883 35.62041718887304 23.181401289789726 16.429212824908404 4 14.469318821767828 25.449774077307225 24.229803543918337 35.8511035570066 5 7.961655493962056 47.80932718158993 33.35086856443507 10.878148760012946 6 32.38229797000686 27.59084912557004 19.569849362048455 20.45700354237465 7 19.847768534228972 39.03455097299338 20.983562674842016 20.134117817935632 8 29.010338687775473 36.319878591556396 23.079490593607087 11.590292127061044 9 28.774609120570062 22.619650076990265 15.735549692489172 32.870191109950504 10 18.117070130768358 38.36542254311765 27.915361094417264 15.602146231696723 11 23.48156582587489 34.01878381209225 12.571420682601357 29.928229679431507 12 24.258686004848954 41.65170502669084 22.157573531133117 11.932035437327086 13 30.883626887077614 25.523657095928108 15.442233677945557 28.15048233904872 14 20.68002170614111 36.29541721892468 21.654096794022166 21.370464280912042 15 40.75874301114315 21.596235395802633 16.999480806340763 20.645540786713443 16 14.008619396042041 30.85491683723387 36.44149795019238 18.694965816531713 17 30.708260153973775 34.88197464822887 23.359992028801955 11.0497731689954 18 29.859403314943656 24.152401212266 28.043645417597308 17.944550055193037 19 23.086785607741756 39.354757773057166 26.80808101422647 10.750375604974613 20 24.794448519393086 30.244128772645478 23.477286396327504 21.484136311633932 21 14.103632957655007 34.26247544345828 32.567031180880804 19.06686041800591 22 20.575886155310748 35.71500171969923 12.495280581084268 31.21383154390575 23 11.903507076459862 35.49808937384525 25.858281120756615 26.740122428938275 24 18.97317676440218 46.63346780971592 17.7537641380304 16.6395912878515 25 20.38195951450057 28.043481001374087 14.00928425512552 37.56527522899982 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 26807.0 1 26807.0 2 27872.5 3 28938.0 4 28938.0 5 28938.0 6 30843.0 7 32748.0 8 32748.0 9 32748.0 10 29755.5 11 26763.0 12 26763.0 13 26763.0 14 24691.0 15 22619.0 16 22619.0 17 22619.0 18 21600.5 19 20582.0 20 20582.0 21 20582.0 22 20276.5 23 19971.0 24 19971.0 25 19971.0 26 21188.0 27 22405.0 28 22405.0 29 22405.0 30 22766.5 31 23128.0 32 23128.0 33 23128.0 34 23967.5 35 24807.0 36 24807.0 37 24807.0 38 27983.0 39 31159.0 40 31159.0 41 31159.0 42 67885.0 43 104611.0 44 104611.0 45 104611.0 46 404430.5 47 704250.0 48 704250.0 49 704250.0 50 567709.0 51 431168.0 52 431168.0 53 431168.0 54 269677.0 55 108186.0 56 108186.0 57 108186.0 58 63807.5 59 19429.0 60 19429.0 61 19429.0 62 14890.5 63 10352.0 64 10352.0 65 10352.0 66 8133.5 67 5915.0 68 5915.0 69 5915.0 70 4506.0 71 3097.0 72 3097.0 73 3097.0 74 2271.5 75 1446.0 76 1446.0 77 1446.0 78 1036.0 79 626.0 80 626.0 81 626.0 82 416.5 83 207.0 84 207.0 85 207.0 86 136.5 87 66.0 88 66.0 89 66.0 90 50.0 91 34.0 92 34.0 93 34.0 94 23.5 95 13.0 96 13.0 97 13.0 98 9.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3066494222542776 2 0.18180914173505225 3 0.19918134967678705 4 0.22278359081141988 5 0.24644573611136902 6 0.19283150815325642 7 0.19085467069781764 8 0.21271969103827695 9 0.21194093688916474 10 0.2226038783154709 11 0.19912144551147073 12 0.20403358706740957 13 0.21541537847751166 14 0.2063698495147463 15 0.19696489556008298 16 0.19906154134615445 17 0.19978039132995035 18 0.20139780379349118 19 0.20091857047096054 20 0.19930115800741974 21 0.19648566223755237 22 0.2003195288177973 23 0.20289540792639932 24 0.21643374928788922 25 0.20978438693777693 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1669333.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.936415730088335 #Duplication Level Percentage of deduplicated Percentage of total 1 77.33551056525307 9.231088048054993 2 10.375117793017381 2.4768343845218403 3 4.110533434205778 1.471951078293236 4 2.194087547575581 1.047581644642884 5 1.327460284334842 0.7922558909500971 6 0.8969282495146051 0.6423665079760036 7 0.5885910564711353 0.4917967281535958 8 0.45293000495611496 0.4325088668629727 9 0.31094600864055233 0.3340422745870745 >10 1.9049859641048932 4.354516504363567 >50 0.23005951452475842 1.9206978809773123 >100 0.1948206392146049 4.527256280458768 >500 0.024163800212664177 2.0287987284267657 >1k 0.03523887531013526 8.67300490085233 >5k 0.004027300035444029 3.734616881510882 >10k+ 0.014598962628484608 57.84068339936768 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 155606 9.321447548212369 No Hit CCCATGTACTCTGCGTTGATACCAC 145851 8.737082415551601 No Hit GAGTACATGGGAAGCAGTGGTATCA 82683 4.95305610084986 No Hit CATGTACTCTGCGTTGATACCACTG 69270 4.149561531461967 No Hit GCGTTGATACCACTGCTTCCCATGT 51447 3.0818895930290724 No Hit ACGCAGAGTACATGGGAAGCAGTGG 38239 2.290675377531026 No Hit TATCAACGCAGAGTACATGGGAAGC 35309 2.1151561731541877 No Hit ACTCTGCGTTGATACCACTGCTTCC 32698 1.9587463975132582 No Hit GTATCAACGCAGAGTACATGGGAAG 32622 1.9541936809492173 No Hit GCTTCCCATGTACTCTGCGTTGATA 31755 1.902256769619962 No Hit GGTATCAACGCAGAGTACATGGGAA 29914 1.791973201272604 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 20660 1.2376200554353145 No Hit GCAGTGGTATCAACGCAGAGTACAT 18089 1.083606446407038 No Hit CAGTGGTATCAACGCAGAGTACATG 17083 1.0233428560988131 No Hit GCAGAGTACATGGGAAGCAGTGGTA 16372 0.9807509945589047 No Hit GTTGATACCACTGCTTCCCATGTAC 15876 0.9510385285620065 No Hit GTACTCTGCGTTGATACCACTGCTT 15455 0.925818874963833 No Hit GTGGTATCAACGCAGAGTACATGGG 15118 0.9056311712522306 No Hit ATACCACTGCTTCCCATGTACTCTG 15006 0.8989219047368021 No Hit GGGAAGCAGTGGTATCAACGCAGAG 13965 0.8365616686425057 No Hit AAGCAGTGGTATCAACGCAGAGTAC 13586 0.8138579899876178 No Hit ACATGGGAAGCAGTGGTATCAACGC 13541 0.8111623025483831 No Hit CATGGGAAGCAGTGGTATCAACGCA 12936 0.7749202825320053 No Hit CTGCTTCCCATGTACTCTGCGTTGA 12797 0.7665936035530359 No Hit TACCACTGCTTCCCATGTACTCTGC 12633 0.7567693204411583 No Hit CCACTGCTTCCCATGTACTCTGCGT 12273 0.7352038209272805 No Hit GATACCACTGCTTCCCATGTACTCT 11163 0.6687101974261576 No Hit GTACATGGTAAGCAGTGGTATCAAC 10472 0.6273164191925756 No Hit CTTCCCATGTACTCTGCGTTGATAC 10158 0.6085065112832491 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 9419 0.5642373331144834 No Hit ATGGGAAGCAGTGGTATCAACGCAG 9225 0.5526159250431161 No Hit CCATGTACTCTGCGTTGATACCACT 9089 0.5444689585600956 No Hit TTCCCATGTACTCTGCGTTGATACC 8527 0.5108028176523198 No Hit ACCATGTACTCTGCGTTGATACCAC 8123 0.48660153486452373 No Hit TGATACCACTGCTTCCCATGTACTC 7419 0.4444290024818296 No Hit GTACATGGGTAAGCAGTGGTATCAA 5292 0.31701284285400216 No Hit ATCAACGCAGAGTACATGGGAAGCA 5057 0.30293536400466536 No Hit CCCCATGTACTCTGCGTTGATACCA 4909 0.2940695475378489 No Hit GGTAAGCAGTGGTATCAACGCAGAG 4653 0.27873408121686927 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 4542 0.27208471886675695 No Hit GAGTACATGGTAAGCAGTGGTATCA 4353 0.26076283162197117 No Hit TCCATGTACTCTGCGTTGATACCAC 4295 0.2572883900336242 No Hit GGAAGCAGTGGTATCAACGCAGAGT 4195 0.2512979735019915 No Hit GCTTACCATGTACTCTGCGTTGATA 3714 0.22248406998483825 No Hit GTACATGGAAAGCAGTGGTATCAAC 3410 0.20427320372867486 No Hit AAACAAAAAAAAAAAAAAAAAAAAA 3330 0.1994808705033687 No Hit AAAAAGTACTCTGCGTTGATACCAC 3191 0.19115419152439928 No Hit CTGCGTTGATACCACTGCTTCCCAT 3081 0.1845647333396033 No Hit GCGTTGATACCACTGCTTACCATGT 2937 0.17593853353405223 No Hit CTCTGCGTTGATACCACTGCTTCCC 2805 0.16803118371229706 No Hit GAGTACATGGAAAGCAGTGGTATCA 2752 0.16485626295053174 No Hit TGGGAAGCAGTGGTATCAACGCAGA 2585 0.15485226734270513 No Hit ACTCTGCGTTGATACCACTGCTTAC 2525 0.15125801742372552 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2404 0.14400961342044996 No Hit GTACATGGAAGCAGTGGTATCAACG 2399 0.14371009259386833 No Hit AAAGTACTCTGCGTTGATACCACTG 2359 0.14131392598121525 No Hit AGTGGTATCAACGCAGAGTACATGG 2330 0.13957670518704177 No Hit GCTTTCCATGTACTCTGCGTTGATA 2286 0.13694092191312338 No Hit TCCCATGTACTCTGCGTTGATACCA 2239 0.134125426143256 No Hit ACTGCTTCCCATGTACTCTGCGTTG 2216 0.1327476303409805 No Hit ACGCAGAGTACATGGTAAGCAGTGG 2164 0.1296326137445315 No Hit AAAAACAAAAAAAAAAAAAAAAAAA 2152 0.12891376376073557 No Hit TATCAACGCAGAGTACATGGTAAGC 2069 0.12394171803948044 No Hit TAAAAAAAAAAAAAAAAAAAAAAAA 2061 0.12346248471694983 No Hit CTGCTTACCATGTACTCTGCGTTGA 2050 0.12280353889847022 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 2014 0.12064698894708246 No Hit ATGGTAAGCAGTGGTATCAACGCAG 2001 0.11986823479797021 No Hit TCAACGCAGAGTACATGGGAAGCAG 1979 0.11855034316101101 No Hit AACGCAGAGTACATGGGAAGCAGTG 1933 0.11579475155645998 No Hit GTACAAAAAAAAAAAAAAAAAAAAA 1909 0.11435705158886814 No Hit GTATCAACGCAGAGTACTTTTTTTT 1897 0.1136382016050722 No Hit GAAAACAAAAAAAAAAAAAAAAAAA 1890 0.11321887244785793 No Hit TTGATACCACTGCTTCCCATGTACT 1845 0.1105231850086232 No Hit GTATCAACGCAGAGTACATGGTAAG 1821 0.10908548504103135 No Hit GGTATCAACGCAGAGTACATGGTAA 1805 0.10812701839597012 No Hit GCGTTGATACCACTGCTTTCCATGT 1715 0.1027356435175007 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.990416531632694E-5 0.0 2 0.0 0.0 0.0 5.990416531632694E-5 0.0 3 0.0 0.0 0.0 5.990416531632694E-5 0.0 4 0.0 0.0 0.0 5.990416531632694E-5 0.0 5 0.0 0.0 0.0 5.990416531632694E-5 0.0 6 0.0 0.0 0.0 5.990416531632694E-5 0.0 7 0.0 0.0 0.0 5.990416531632694E-5 0.0 8 5.990416531632694E-5 0.0 0.0 1.1980833063265388E-4 0.0 9 5.990416531632694E-5 0.0 0.0 1.1980833063265388E-4 0.0 10 1.1980833063265388E-4 0.0 0.0 1.7971249594898083E-4 0.0 11 1.1980833063265388E-4 0.0 0.0 3.5942499189796167E-4 0.0 12 1.1980833063265388E-4 0.0 0.0 4.792333225306155E-4 0.0 13 1.1980833063265388E-4 0.0 0.0 5.391374878469425E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAA 835 0.0 16.28637 1 GTACAAG 190 0.0 16.016628 1 GTATCTA 55 1.1311124E-5 15.541525 18 TACATAA 570 0.0 15.507719 2 GTACATA 680 0.0 15.243767 1 TACAAAA 880 0.0 15.22917 2 AATAAGC 50 8.7288776E-5 15.19707 6 AGAACAA 965 0.0 15.069677 2 TACAAGA 165 0.0 14.977151 2 CGGCGTT 45 6.7664176E-4 14.776704 9 GGTGTCC 45 6.7664176E-4 14.776704 8 CTCTCTT 65 3.381334E-6 14.6116905 19 GTAAATA 85 1.6934791E-8 14.544512 1 GTAAAAA 490 0.0 14.36185 1 GTCTCAC 40 0.0052430583 14.264809 1 GTATATA 60 2.5434425E-5 14.264809 1 AAGCATA 60 2.5702391E-5 14.248965 9 TATCTAC 60 2.5746082E-5 14.246399 19 GTCGAAA 55 1.9644934E-4 13.814689 15 TCGAAAC 50 0.0015024856 13.2966385 16 >>END_MODULE