FastQCFastQC Report
Wed 25 May 2016
SRR1295058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295058_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669333
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1664839.973025154358057No Hit
GTACATGGGAAGCAGTGGTATCAAC1540359.22733810450042No Hit
GAGTACATGGGAAGCAGTGGTATCA894025.355552187610261No Hit
CATGTACTCTGCGTTGATACCACTG784664.70044023571091No Hit
GCGTTGATACCACTGCTTCCCATGT512893.0724247349090925No Hit
ACGCAGAGTACATGGGAAGCAGTGG499432.9917937283933163No Hit
TATCAACGCAGAGTACATGGGAAGC398412.3866418503677815No Hit
ACTCTGCGTTGATACCACTGCTTCC375912.2518574784060457No Hit
GTATCAACGCAGAGTACATGGGAAG354672.1246210312741676No Hit
GGTATCAACGCAGAGTACATGGGAA339472.033566699993351No Hit
GCTTCCCATGTACTCTGCGTTGATA329841.9758789887937278No Hit
CAGTGGTATCAACGCAGAGTACATG214741.2863820460028046No Hit
ACATGGGAAGCAGTGGTATCAACGC181801.0890577254508238No Hit
GCAGTGGTATCAACGCAGAGTACAT176761.058866026131395No Hit
CATGGGAAGCAGTGGTATCAACGCA173621.0400561182220684No Hit
GTGGTATCAACGCAGAGTACATGGG166550.9977038733434251No Hit
AAGCAGTGGTATCAACGCAGAGTAC165490.9913540318198945No Hit
GGGAAGCAGTGGTATCAACGCAGAG165030.9885984402153434No Hit
ATACCACTGCTTCCCATGTACTCTG161900.9698484364713331No Hit
GCAGAGTACATGGGAAGCAGTGGTA152690.914676700214996No Hit
GTTGATACCACTGCTTCCCATGTAC152540.9137781377352512No Hit
GTACTCTGCGTTGATACCACTGCTT149750.897064875611996No Hit
CCACTGCTTCCCATGTACTCTGCGT146630.8783747760333018No Hit
CTGCTTCCCATGTACTCTGCGTTGA142530.853814068253608No Hit
TACCACTGCTTCCCATGTACTCTGC136610.8183508023863423No Hit
GATACCACTGCTTCCCATGTACTCT129590.7762980783342808No Hit
GTACTTTTTTTTTTTTTTTTTTTTT121950.730531296032607No Hit
CTTCCCATGTACTCTGCGTTGATAC117150.70177729668077No Hit
ATGGGAAGCAGTGGTATCAACGCAG114070.6833268137633414No Hit
CCATGTACTCTGCGTTGATACCACT110950.6646367141846474No Hit
GTATCAACGCAGAGTACTTTTTTTT109080.6534346352704943No Hit
TATCAACGCAGAGTACTTTTTTTTT107920.6464857520938003No Hit
GTACATGGTAAGCAGTGGTATCAAC107560.6443292021424125No Hit
GGTATCAACGCAGAGTACTTTTTTT100740.6034745613966777No Hit
ACCATGTACTCTGCGTTGATACCAC89680.5372205545568199No Hit
TTCCCATGTACTCTGCGTTGATACC87540.524401063179126No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA85790.5139178342487688No Hit
ACGCAGAGTACTTTTTTTTTTTTTT77510.4643171853668501No Hit
TGATACCACTGCTTCCCATGTACTC72020.43142979860818664No Hit
GAGTACTTTTTTTTTTTTTTTTTTT67750.40585072001811506No Hit
ATCAACGCAGAGTACATGGGAAGCA63990.3833267538591761No Hit
CCCCATGTACTCTGCGTTGATACCA59440.35607035864024733No Hit
GTACATGGGTAAGCAGTGGTATCAA57750.3459465547017881No Hit
GGTAAGCAGTGGTATCAACGCAGAG55250.33097051337270633No Hit
GGAAGCAGTGGTATCAACGCAGAGT51770.3101238638426246No Hit
GAGTACATGGTAAGCAGTGGTATCA50280.3011981432104919No Hit
TCCATGTACTCTGCGTTGATACCAC44350.26567497317791No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA43060.25794733585210383No Hit
GCTTACCATGTACTCTGCGTTGATA38640.2314696947822873No Hit
CTGCGTTGATACCACTGCTTCCCAT36380.21793135342079742No Hit
GTACATGGAAAGCAGTGGTATCAAC34940.20930515361524635No Hit
AAAAAGTACTCTGCGTTGATACCAC33610.20133789962817486No Hit
CTCTGCGTTGATACCACTGCTTCCC32510.19474844144337888No Hit
GAGTACATGGAAAGCAGTGGTATCA31670.18971649155680742No Hit
TGGGAAGCAGTGGTATCAACGCAGA30550.1830072250413788No Hit
ACTCTGCGTTGATACCACTGCTTAC29240.17515977938493998No Hit
GCGTTGATACCACTGCTTACCATGT29200.17492016272367467No Hit
ACGCAGAGTACATGGTAAGCAGTGG29100.1743211210705114No Hit
AGTGGTATCAACGCAGAGTACATGG27990.1676717587203991No Hit
TCAACGCAGAGTACATGGGAAGCAG26040.15599044648371535No Hit
ATGGTAAGCAGTGGTATCAACGCAG25530.15293533405258267No Hit
AAAGTACTCTGCGTTGATACCACTG25230.15113820909309286No Hit
ACTGCTTCCCATGTACTCTGCGTTG25140.15059907160524594No Hit
TCCCATGTACTCTGCGTTGATACCA24960.14952079662955206No Hit
GTACATGGAAGCAGTGGTATCAACG24810.14862223414980713No Hit
AACGCAGAGTACATGGGAAGCAGTG24080.14424923008171528No Hit
GCAGAGTACTTTTTTTTTTTTTTTT23950.143470475932603No Hit
GCTTTCCATGTACTCTGCGTTGATA23850.14287143427943974No Hit
CTGCTTACCATGTACTCTGCGTTGA23090.13831871771539891No Hit
TATCAACGCAGAGTACATGGTAAGC21950.13148964286933762No Hit
GTATCAACGCAGAGTACATGGTAAG19560.11717254735873549No Hit
ACGCAGAGTACATGGAAAGCAGTGG18830.11279954329064364No Hit
CTGCTTACCCATGTACTCTGCGTTG18730.11220050163748035No Hit
GAGTACATGGGTAAGCAGTGGTATC18640.11166136414963342No Hit
GGTATCAACGCAGAGTACATGGTAA18310.10968452669419464No Hit
GTGGTATCAACGCAGAGTACTTTTT18310.10968452669419464No Hit
CGTTGATACCACTGCTTCCCATGTA18290.10956471836356196No Hit
ATGGAAAGCAGTGGTATCAACGCAG17650.10573085178331705No Hit
TTGATACCACTGCTTCCCATGTACT17290.10357430183192927No Hit
GCGTTGATACCACTGCTTTCCATGT17230.1032148768400313No Hit
CAACGCAGAGTACATGGGAAGCAGT17050.10213660186433743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGCC250.006034349618.99886113
GTACAAA4550.017.9754281
GGACGCA1250.016.7198
TATTACA350.002171158816.2847392
TACAAAA4300.015.9060252
TACAAAG901.3460522E-1015.8323852
GGGACGC1350.015.4805557
CGCAAAA2450.015.1215422
TGGGACG1450.015.0680636
GTATTAC400.005241662714.2653841
ACGCCGA400.005281417614.2491467
CGAGTAC400.005281417614.24914611
CAACGCC400.005281417614.2491465
CCGAGTA400.005281417614.24914610
GACGCAG1500.013.9324999
GTACAAG1301.8189894E-1213.8996051
AGAACAA1550.013.4830642
TCTAGAA500.001500306113.2992032
AAAAAGT5400.013.2086881
ACGCAGT1800.013.19365410
Produced by FastQC (version 0.11.5)