FastQCFastQC Report
Wed 25 May 2016
SRR1295057_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295057_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2616327
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC80820.3089063408358359No Hit
CCCATGTACTCTGCGTTGATACCAC72940.2787877814967319No Hit
GAGTACATGGGAAGCAGTGGTATCA41880.160071734152497No Hit
GTATCAACGCAGAGTACTTTTTTTT37340.14271916316270863No Hit
GGTATCAACGCAGAGTACTTTTTTT32870.12563414282694785No Hit
CATGTACTCTGCGTTGATACCACTG30680.11726362950808518No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN30340.11596409775995126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT707.2816892E-613.5687886
TAACGCT550.003057166712.0973624
GACCGTG550.003072118612.0894827
ACCGTGA802.8715753E-511.8735998
AGAACGG951.3530407E-511.0058715
GCAATAC1502.4288966E-810.1381583
GTCTAGT2105.456968E-129.9634211
CTACACT3750.09.8852734
TACACTG6450.09.7261185
GTCTTAG2800.09.5105371
AATACCG900.00110862459.505075
ATTATAC4350.09.3952753
GTGCTAG3200.09.2133331
TAGACAG3750.09.1248675
TAGACTG3350.09.079475
ACAGCGT1054.4993538E-49.0465528
GTCTTGC4700.08.90348151
GTCCTAA2252.564775E-108.8765021
CCAGAAC3250.08.7734053
GTGTAGA4150.08.7084451
Produced by FastQC (version 0.11.5)