FastQCFastQC Report
Wed 25 May 2016
SRR1295057_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295057_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2616327
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT101800.3890950940001001No Hit
GGTATCAACGCAGAGTACTTTTTTT94830.36245469316335455No Hit
GTACATGGGAAGCAGTGGTATCAAC80600.3080654673517492No Hit
CCCATGTACTCTGCGTTGATACCAC80280.30684237864762315No Hit
TATCAACGCAGAGTACTTTTTTTTT67540.25814815961460474No Hit
GAGTACATGGGAAGCAGTGGTATCA45330.17325815924385599No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44530.17020043748354086No Hit
CATGTACTCTGCGTTGATACCACTG34830.13312556113971993No Hit
ACGCAGAGTACATGGGAAGCAGTGG28830.11019264793735645No Hit
GCTTCCCATGTACTCTGCGTTGATA26400.10090481809039925No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGA350.002171739716.28458410
AGTACCG500.001500809713.2990785
ATCCCGT604.0960847E-412.6657886
ACAGCGG700.001492856710.8563898
GTAGGAC2200.010.3629173
GGGTTAG1752.3101165E-1010.326991
GGAACGA856.600362E-410.058125515
GTCTTAC2400.09.9080221
GTATTAG4050.09.8639861
GGTCTAC1002.7225836E-49.5117031
GCTCTAG1304.1749063E-69.5117021
CTAGGAC3150.09.3485573
TAGACAG3800.08.9993745
TAGGACC1701.6876584E-78.9405564
ATTAGAC2701.8189894E-128.7956863
GTCTTGG3250.08.7800321
CCTGCAC4450.08.7522023
TTACACT4400.08.6357644
GTCCTAT2002.4869223E-88.5605321
TAACCCG1451.691254E-58.516655
Produced by FastQC (version 0.11.5)