FastQCFastQC Report
Wed 25 May 2016
SRR1295056_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295056_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2323733
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC58110.25007175953519617No Hit
CCCATGTACTCTGCGTTGATACCAC50070.21547225950657845No Hit
GTATCAACGCAGAGTACTTTTTTTT31980.1376233844421885No Hit
GGTATCAACGCAGAGTACTTTTTTT31220.1343527849369958No Hit
GAGTACATGGGAAGCAGTGGTATCA29590.1273382096824377No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN27250.11726820594276538No Hit
ATTCCATTCCATTCCATTCCATTCC24140.10388456849388461No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACCG657.9900236E-411.6985775
GTATTCG752.0540279E-411.412271
GTCTAGG3000.09.8272321
TAGACAG4050.09.6224395
TAGAACC2002.6193447E-109.50534
TAGGACT2401.8189894E-129.5052994
TACACTA3200.09.5050935
CTGTCGC1804.1927706E-99.4987389
ACGCTGT800.0045218169.4987386
GTTCTAG2850.09.0096871
TAGACTG2850.09.0048265
TCTGTCG1901.0604708E-88.9988048
GTCTAAG2553.6379788E-128.95081
GTAGGAC1606.698956E-78.9102553
AATACTG4300.08.8419485
TTTAGAC2502.5465852E-118.7439313
GTCCTAG2850.08.6759951
GTCTTAC2201.5734258E-98.6456591
CGTTCCG1651.0528584E-68.63484410
CTGTGCA4850.08.6174129
Produced by FastQC (version 0.11.5)