FastQCFastQC Report
Wed 25 May 2016
SRR1295056_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295056_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2323733
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTT90460.3892874095259653No Hit
GTATCAACGCAGAGTACTTTTTTTT88300.3799920214585755No Hit
TATCAACGCAGAGTACTTTTTTTTT67180.28910378257743036No Hit
GTACATGGGAAGCAGTGGTATCAAC57520.24753274149827026No Hit
CCCATGTACTCTGCGTTGATACCAC56060.24124974771197893No Hit
ACGCAGAGTACTTTTTTTTTTTTTT41240.1774730573607209No Hit
GAGTACATGGGAAGCAGTGGTATCA34610.14894138009831592No Hit
CATGTACTCTGCGTTGATACCACTG24770.10659572334687333No Hit
ATTCCATTCCATTCCATTCCATTCC23240.1000114901324722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCCG500.00150078213.29900710
CCCCTAA1252.52857E-69.8928851
TGGACTG2800.09.838555
GAACTGT3400.09.49928956
GCACCGT900.00111471239.49928956
TATTCCG800.0045196559.49928955
GGAACGA800.0045196559.499289515
TTACACC2151.07320375E-109.2783764
CTCTTAG3000.09.195311
CCCTTAC2600.09.1465291
GTGTAGG2304.1836756E-119.0988081
CCGGGCA950.00182540678.9993289
CTTACAC3550.08.8303263
GTATTAA4000.08.7989611
GTCTTAT2502.5465852E-118.7513981
GTTACAC1856.584014E-88.7290773
GTTCTAG2950.08.7062551
ACAGTGC4500.08.6549098
GGGTAGC1107.053632E-48.6476271
GGACTGC2201.6079866E-98.6357186
Produced by FastQC (version 0.11.5)