FastQCFastQC Report
Wed 25 May 2016
SRR1295055_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295055_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3217211
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC120890.37576024699654453No Hit
CCCATGTACTCTGCGTTGATACCAC108950.33864735635928134No Hit
GAGTACATGGGAAGCAGTGGTATCA57030.17726533945084733No Hit
ATTCCATTCCATTCCATTCCATTCC56320.1750584590193183No Hit
GTATCAACGCAGAGTACTTTTTTTT53240.16548494954169932No Hit
GGTATCAACGCAGAGTACTTTTTTT47130.1464933447013578No Hit
CATGTACTCTGCGTTGATACCACTG40500.1258854330660936No Hit
GAATGGAATGGAATGGAATGGAATG40500.1258854330660936No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN38520.11973103411619568No Hit
GCTTCCCATGTACTCTGCGTTGATA35540.11046835286836953No Hit
GCGTTGATACCACTGCTTCCCATGT34550.10739115339342058No Hit
TATCAACGCAGAGTACTTTTTTTTT34430.10701815951766919No Hit
GGTATCAACGCAGAGTACATGGGAA33690.10471803061720228No Hit
GTATCAACGCAGAGTACATGGGAAG32950.1024179017167354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG500.001493974613.3072874
CTAGCAC3300.010.0808083
GTCCTAG2201.8189894E-129.9426841
GTTAGAC1151.0211323E-59.9180123
CCGTACC1701.6518243E-89.5052064
TAGAACG900.00110862529.5052054
TATACTG6350.09.1304165
GTCCTAC2401.0913936E-119.1141271
GTACTAG2151.020453E-98.8468781
GTCTTAG3300.08.6458121
GTCTTAC3300.08.6458121
AGTGCGA1107.154033E-48.63557810
GTGTAGA5200.08.5959331
TCCGTAC2002.5191184E-88.5542853
TATTCGA1000.00291388068.54749116
TTATACT6350.08.5322324
GGAACGA1451.6964634E-58.51474215
GAGGCCG2159.777068E-98.39288615
GTACTAC2951.8189894E-128.3820421
AGGTGCG1252.748283E-48.3589797
Produced by FastQC (version 0.11.5)