FastQCFastQC Report
Wed 25 May 2016
SRR1295055_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295055_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3217211
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT135320.420612760555649No Hit
GGTATCAACGCAGAGTACTTTTTTT125330.38956102039934587No Hit
GTACATGGGAAGCAGTGGTATCAAC121410.37737655379146723No Hit
CCCATGTACTCTGCGTTGATACCAC118990.36985451063048086No Hit
TATCAACGCAGAGTACTTTTTTTTT93920.29192987342141996No Hit
GAGTACATGGGAAGCAGTGGTATCA63870.1985259903686765No Hit
ATTCCATTCCATTCCATTCCATTCC54740.17014737298859167No Hit
ACGCAGAGTACTTTTTTTTTTTTTT50670.15749666403602375No Hit
CATGTACTCTGCGTTGATACCACTG44320.13775907144417945No Hit
GAATGGAATGGAATGGAATGGAATG44220.13744824321438662No Hit
GCTTCCCATGTACTCTGCGTTGATA39660.12327447593583386No Hit
ACGCAGAGTACATGGGAAGCAGTGG37080.11525510760717901No Hit
GGTATCAACGCAGAGTACATGGGAA36920.1147577824395105No Hit
GCGTTGATACCACTGCTTCCCATGT36250.11267523329989858No Hit
GTATCAACGCAGAGTACATGGGAAG34740.10798172703002694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCGC500.001500933313.2990866
TATACCG550.003071253412.0900775
ATACCGT600.00588098111.0825726
AGAAGCG700.001493009710.8563975
AGCCGTG856.5983715E-410.0586017
TCGTTCC1151.02807335E-59.91236219
TAGACTG4750.09.7993275
GTGTAGG3800.09.7628351
GTCTAGG3450.09.6503681
GTCTTAG3300.09.5125061
CCTACAC4350.09.3901593
TATACTG7800.09.3775625
GTGTTAG5800.09.3484971
TAGACAG5400.09.3234345
GTCTAGA4300.09.2912841
TACACTG8700.09.2809715
TCGCTCT1752.6755515E-89.2279374
GTTCAGG3750.09.1320051
CCGCGCC1151.1121036E-49.08633119
CTGTTCG1054.4978608E-49.0469989
Produced by FastQC (version 0.11.5)