FastQCFastQC Report
Wed 25 May 2016
SRR1295054_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295054_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164531
Sequences flagged as poor quality0
Sequence length25
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA12230.743324966115808No Hit
GGTATCAACGCAGAGTACTTTTTTT5520.33549908527876204No Hit
GTATCAACGCAGAGTACTTTTTTTT4950.30085515799454204No Hit
TATCAACGCAGAGTACTTTTTTTTT3700.22488163324844557No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2320.14100686192875508No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN1930.11730312220797295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACATC250.006012920818.9981698
CTCCAGG350.002134146216.3140221
TATTCTG350.002138513816.3090345
ATGTACT456.6441944E-414.798944
CCATGTA456.6750916E-414.7898972
GTCTTAG400.00519223814.2747721
GTGCAGA603.9983194E-412.6886851
TTAATAT752.0367198E-411.4093493
GGTATCA1502.3064786E-810.1509481
ATCAACG2601.9274812E-66.5863424
TCAACGC2756.8863847E-76.57303525
GTATCAA2402.8144723E-56.34434271
CAACGCA2703.6680885E-66.3327236
AACGCAG2901.1704105E-55.8959837
TATCAAC2651.2033665E-45.74055343
CGCAGAG3002.0194026E-55.69945059
ACGCAGA3052.6314323E-55.60601668
Produced by FastQC (version 0.11.5)