##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295054_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	164531
Sequences flagged as poor quality	0
Sequence length	25
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.90993186694301	33.0	33.0	33.0	27.0	33.0
2	30.868334842674024	33.0	33.0	33.0	27.0	33.0
3	30.838990828476092	33.0	33.0	33.0	27.0	33.0
4	34.350177170259705	37.0	37.0	37.0	27.0	37.0
5	34.59240507867818	37.0	37.0	37.0	27.0	37.0
6	34.57469413058937	37.0	37.0	37.0	27.0	37.0
7	34.474640037439755	37.0	37.0	37.0	27.0	37.0
8	34.3623876351569	37.0	37.0	37.0	27.0	37.0
9	34.24773446949207	37.0	37.0	37.0	27.0	37.0
10	34.246883566014915	37.0	37.0	37.0	27.0	37.0
11	34.343552278901846	37.0	37.0	37.0	27.0	37.0
12	34.299378232673476	37.0	37.0	37.0	27.0	37.0
13	34.2165184676444	37.0	37.0	37.0	27.0	37.0
14	35.62411946684819	40.0	37.0	40.0	27.0	40.0
15	35.844922841288266	40.0	37.0	40.0	27.0	40.0
16	35.915474895308485	40.0	37.0	40.0	27.0	40.0
17	35.84665503765248	40.0	37.0	40.0	27.0	40.0
18	35.77443764396983	40.0	37.0	40.0	27.0	40.0
19	35.920531693115585	40.0	37.0	40.0	27.0	40.0
20	35.93187302088968	40.0	37.0	40.0	27.0	40.0
21	35.94360941099246	40.0	37.0	40.0	27.0	40.0
22	35.86505886428697	40.0	37.0	40.0	27.0	40.0
23	35.76589214190639	40.0	37.0	40.0	27.0	40.0
24	35.839525682090304	40.0	37.0	40.0	27.0	40.0
25	34.89879718715622	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4384134919468572
1101	2	0.5411485315773135
1101	3	0.6562410280034747
1101	4	0.6789350086875459
1101	5	0.43102492767283707
1101	6	0.3794399190092932
1101	7	0.6130602108103105
1101	8	0.7227116772697642
1101	9	0.500836279487352
1101	10	0.8858892621000507
1101	11	0.8433795138307403
1101	12	0.5999807735700884
1101	13	0.7611694065915628
1101	14	1.0314174012219937
1101	15	0.7569358851403862
1101	16	0.8095200798894879
1101	17	0.8744581460939429
1101	18	0.8829833534744154
1101	19	0.9337744277954414
1101	20	1.0181401514072306
1101	21	0.3328754914519081
1101	22	0.5410397042337394
1101	23	0.6837675456616807
1101	24	0.957963901783792
1101	25	0.7432350134829662
1102	1	0.6961170495279738
1102	2	0.7123273629493312
1102	3	0.724042538104829
1102	4	0.48564883767876665
1102	5	0.475443674696848
1102	6	0.42877329769101635
1102	7	0.5875517419986664
1102	8	0.389673108464919
1102	9	0.17987171925335588
1102	10	0.36750615890979077
1102	11	0.38150617188121316
1102	12	0.69126708062403
1102	13	0.6628597677914883
1102	14	0.7485624933925976
1102	15	0.7155781543737731
1102	16	0.4393751917866382
1102	17	1.0698870284828956
1102	18	1.0262049030288694
1102	19	0.874288678526689
1102	20	0.928537403294861
1102	21	0.7431877151101602
1102	22	1.3595044345241973
1102	23	1.0874305617830302
1102	24	0.9598005115382264
1102	25	1.2836914450182348
1103	1	-0.32774304206292015
1103	2	-0.6186277044164363
1103	3	-0.5215438427274535
1103	4	-0.23735507816380164
1103	5	-0.8658319607979408
1103	6	-0.32066255549806755
1103	7	-0.6519119866799059
1103	8	-0.8016722157535696
1103	9	0.1986876746525752
1103	10	-0.028972901287993125
1103	11	-0.263479558522981
1103	12	-0.24800417443670142
1103	13	-0.22658404982316682
1103	14	-0.4464497850968172
1103	15	-0.46647534001794355
1103	16	-0.42380261401411445
1103	17	-0.686799770481727
1103	18	-0.3360769048957337
1103	19	-0.07848891488939103
1103	20	-0.24390900275810168
1103	21	-0.54067768609778
1103	22	-0.6516291687146705
1103	23	-0.47012900548318015
1103	24	-0.29751680445035333
1103	25	-0.4157012675323699
1104	1	0.6387400003476493
1104	2	0.6590486744247386
1104	3	0.4494523741704022
1104	4	0.5963045753836838
1104	5	0.26232892059848467
1104	6	0.5804126419533162
1104	7	0.44001075839210557
1104	8	0.6724599937108167
1104	9	0.8397077848271266
1104	10	0.7609487818606055
1104	11	0.7872438767992449
1104	12	0.7609392117715714
1104	13	0.8595810792668956
1104	14	1.2444641327368586
1104	15	0.9737748756852511
1104	16	0.6893751917866382
1104	17	0.524805061269781
1104	18	1.0303032636846012
1104	19	0.9111739244283257
1104	20	0.9572259278850268
1104	21	0.6981057478970456
1104	22	1.4783568935405924
1104	23	1.259561709324018
1104	24	1.2794726426857679
1104	25	0.7959865269854447
1105	1	0.3833368554831438
1105	2	0.3630132110590658
1105	3	0.5830087475394237
1105	4	0.3025441203820094
1105	5	0.3444299577346541
1105	6	0.31025539872815244
1105	7	0.522044883517566
1105	8	0.4029216865846905
1105	9	0.28415408793853913
1105	10	0.6138594543263167
1105	11	0.08040212370456601
1105	12	0.2504507540934142
1105	13	0.2877677637767633
1105	14	0.5224835372199621
1105	15	0.488411878027172
1105	16	0.7152868679325053
1105	17	0.5761433014839028
1105	18	0.44323400975352456
1105	19	0.4547838274059117
1105	20	0.5086812641178753
1105	21	0.642351314976338
1105	22	-6.996471084548261E-4
1105	23	0.8429173466609186
1105	24	0.5644843522876428
1105	25	0.4092016490998702
1106	1	0.4042552228300842
1106	2	0.050003006977433984
1106	3	-0.23548615041976007
1106	4	0.24106452854528015
1106	5	0.02886873324485606
1106	6	-0.1482905196391897
1106	7	-0.3768071572987566
1106	8	0.2248604620948953
1106	9	0.4433377614079248
1106	10	-0.2982578926124617
1106	11	-0.34434277425462056
1106	12	-0.6480186336616853
1106	13	-0.14985978724364202
1106	14	-0.6988174831882006
1106	15	-0.4223024076871127
1106	16	0.15380727609577605
1106	17	0.2723167708716545
1106	18	-0.27041394943015007
1106	19	-0.4936855603491921
1106	20	0.08164044779135082
1106	21	-0.11798031767672512
1106	22	0.047004434524197336
1106	23	-0.20670000027785562
1106	24	0.3350200665733567
1106	25	0.6363700164468042
1107	1	-0.804261260686399
1107	2	-1.0773321578577324
1107	3	-0.37154384272745133
1107	4	-0.6326992077184599
1107	5	-1.0306092887331673
1107	6	-0.5427273328260043
1107	7	-0.700090124331723
1107	8	-1.1990406368062025
1107	9	-0.6094094913393278
1107	10	-0.8259364640410283
1107	11	-0.7812933237051638
1107	12	-0.5471944578375059
1107	13	-0.7462196773535368
1107	14	-0.8600125381332546
1107	15	-0.2516980120827199
1107	16	-0.1622641524756574
1107	17	-0.07809531704042882
1107	18	-0.8785870263532303
1107	19	-0.8697844614480914
1107	20	-0.9362166950657951
1107	21	-0.8771149330613426
1107	22	-0.5289571039373442
1107	23	-0.4490763739042336
1107	24	-0.3118892740859849
1107	25	-0.5970777857509972
1108	1	0.05764350121836159
1108	2	-0.030812011337584266
1108	3	-0.019712340895949865
1108	4	0.28067075565150645
1108	5	-0.04416240228628254
1108	6	0.05489171479304389
1108	7	-0.05869818293978568
1108	8	0.1630472752817056
1108	9	-0.2050138869437248
1108	10	-0.3198925079970749
1108	11	-0.15821640062824116
1108	12	0.2736846630416139
1108	13	-0.15775813889199242
1108	14	-0.4210015491222663
1108	15	-0.0756906860753972
1108	16	-0.45676964698115086
1108	17	-0.4138096027547178
1108	18	-0.33646248422868297
1108	19	-0.010626952290586189
1108	20	-1.1486709075200068
1108	21	-0.3207046766510828
1108	22	-0.43994611492635016
1108	23	-0.019772344600205827
1108	24	-0.4714130836097894
1108	25	-1.3912169798901886
1109	1	0.602006545581407
1109	2	0.6340638535382794
1109	3	0.5929860718024607
1109	4	0.7286968036775505
1109	5	0.7733793152554398
1109	6	0.7252213851227154
1109	7	0.34207511783351663
1109	8	0.5207599329943662
1109	9	0.4401631582333181
1109	10	0.5685079804034103
1109	11	0.2517551093432644
1109	12	0.23571152506847426
1109	13	0.41346693233307263
1109	14	0.8666256385049209
1109	15	0.5413219309372224
1109	16	0.7155136253021226
1109	17	0.6619331729880571
1109	18	0.9427804950142971
1109	19	0.7733779317143004
1109	20	0.9711907123416097
1109	21	1.1012326452862382
1109	22	1.2613562863760492
1109	23	0.8261373013094442
1109	24	0.9184526062558973
1109	25	1.522580333907122
1110	1	0.2135202331820487
1110	2	-0.0979007197306423
1110	3	-0.03575788955019377
1110	4	-1.6684851608957132
1110	5	-0.4852358216205701
1110	6	-0.10811194821062031
1110	7	-0.04496191920352288
1110	8	0.09209648693961725
1110	9	0.17832740944105296
1110	10	-0.09115385534538234
1110	11	0.2160868323706424
1110	12	0.12604968931967875
1110	13	0.3558984943298569
1110	14	0.48240663689461627
1110	15	0.07795639148472588
1110	16	0.11744599245188425
1110	17	-0.009644926850910451
1110	18	0.12587228245390492
1110	19	0.2767595742263751
1110	20	0.23044997160086922
1110	21	0.31000881275805625
1110	22	-0.3687564350410213
1110	23	-0.03932163588862636
1110	24	-0.15352807007260338
1110	25	-0.14746797527161704
1111	1	0.09033182738494716
1111	2	-0.3442775313248454
1111	3	-0.14083035331831084
1111	4	0.4945583173651542
1111	5	0.3299815696837811
1111	6	0.3773952981661921
1111	7	0.28112503731821903
1111	8	0.1428211246207738
1111	9	0.5768781340787328
1111	10	-0.18173356549030473
1111	11	0.07478248454455638
1111	12	0.11518012410228806
1111	13	-0.14410150567014313
1111	14	0.23240663689461627
1111	15	-0.1865363621384617
1111	16	0.03411265911854855
1111	17	0.13890579778676937
1111	18	0.11862590564231112
1111	19	0.2586436321973906
1111	20	-0.14998481100782612
1111	21	-0.07042596985063909
1111	22	-0.15498831909899025
1111	23	0.14908416121282642
1111	24	0.23777627775348265
1111	25	0.4829668073370783
1112	1	0.5301709737458324
1112	2	0.4417154611898866
1112	3	0.3502510290674188
1112	4	0.6396451146258642
1112	5	0.9521745940507174
1112	6	0.5091041690054965
1112	7	0.7178585936169952
1112	8	0.9542560664905011
1112	9	0.7510300691002314
1112	10	1.2918291037245382
1112	11	1.0139447715329268
1112	12	0.6656260549830009
1112	13	0.5675165863827303
1112	14	0.6083024801817629
1112	15	0.7118551014703911
1112	16	0.6531204629089586
1112	17	0.7839925950474793
1112	18	0.7148195670533681
1112	19	0.6853437436801144
1112	20	0.07477231592321942
1112	21	-0.3463457022921119
1112	22	-1.143242818223058
1112	23	0.5040371792093197
1112	24	0.5212609090642033
1112	25	-0.32528291395612285
1113	1	0.09033182738494716
1113	2	-0.018190574803103488
1113	3	-0.010395570709615498
1113	4	-0.4039924072725256
1113	5	-0.22074306799738252
1113	6	0.2759460228038719
1113	7	-0.17177351340642133
1113	8	0.17905300867874274
1113	9	-0.4376146195444548
1113	10	-0.14187849302653888
1113	11	-0.12086968936849019
1113	12	0.2456149067109834
1113	13	0.0877825523008724
1113	14	-0.7965788703517589
1113	15	-0.6865363621384617
1113	16	-0.5963221234901468
1113	17	0.14252898619256626
1113	18	-0.4538378624736268
1113	19	-1.1543998460634768
1113	20	-1.2007094486889827
1113	21	-1.0269477089810763
1113	22	-0.4412202031569592
1113	23	-1.6770027953089155
1113	24	-0.7187454613769546
1113	25	-0.14746797527161704
1114	1	-0.38698778682929813
1114	2	-0.19348326653553016
1114	3	-0.43065963796424356
1114	4	-0.5707364373926964
1114	5	-0.5339490095106072
1114	6	-0.2807098129793033
1114	7	-0.5470378385392252
1114	8	-0.147343401657011
1114	9	-1.3883856752977195
1114	10	-0.37558059215491113
1114	11	-0.4174553822871516
1114	12	0.150341813720118
1114	13	-0.2595922478435426
1114	14	-0.42369991836867626
1114	15	-0.9508357071278866
1114	16	-1.5103068571019875
1114	17	-1.2318456592249376
1114	18	-1.516966440533352
1114	19	-1.5698802731097388
1114	20	-0.8401312858131291
1114	21	-0.12187266643117312
1114	22	-0.1894279498174427
1114	23	-0.028271555946389526
1114	24	-0.9673916009406227
1114	25	-0.6317889820280271
1115	1	-0.07271165087592024
1115	2	-0.07978477770165426
1115	3	-0.23503325186903368
1115	4	-0.0815286391565877
1115	5	0.22490910591566404
1115	6	0.32304747207923157
1115	7	0.3101105445645942
1115	8	0.11021242896860173
1115	9	0.05513900364395141
1115	10	0.3689910721908518
1115	11	0.35376799179093155
1115	12	0.024600413957365674
1115	13	0.35952168273565377
1115	14	0.2106675064598349
1115	15	-0.994507376631212
1115	16	-0.5999453118959437
1115	17	0.10629710213459731
1115	18	-1.591519021893916
1115	19	-0.397153469251883
1115	20	-0.2659268399933339
1115	21	-0.28057089738687324
1115	22	0.15660588379955698
1115	23	0.232417494546155
1115	24	0.41168932123174073
1115	25	-0.1800766709237962
1116	1	-0.3613253121727631
1116	2	0.47697369565131
1116	3	-0.7694751942444604
1116	4	-0.08815431038471644
1116	5	-0.48347474996516127
1116	6	0.4835215179248351
1116	7	0.07223595596248344
1116	8	-1.7707997541828533
1116	9	-0.32274078156304853
1116	10	0.44517907380757293
1116	11	0.814201619598542
1116	12	0.6633786344088932
1116	13	-0.3940437656158693
1116	14	-0.5850048764722047
1116	15	-0.07163365412991851
1116	16	0.3365406284008401
1116	17	-0.42322250061383215
1116	18	-1.6383173358843308
1116	19	-0.035873949649136705
1116	20	0.039878922464083644
1116	21	0.6159589253511655
1116	22	0.47603829906603323
1116	23	0.6956370799725704
1116	24	0.6651104194475792
1116	25	0.8942492139425369
1201	1	0.12330284187770246
1201	2	0.013693483167909193
1201	3	-0.07506948375309364
1201	4	-0.37500690002615045
1201	5	-0.0020836477075292237
1201	6	-0.4622786148772846
1201	7	-0.14079525253685432
1201	8	0.010574747809179996
1201	9	0.17905204712221234
1201	10	0.2990635359589717
1201	11	0.11101436860252534
1201	12	0.1454337472906957
1201	13	0.006985450851594521
1201	14	0.467551564430849
1201	15	0.04317378278907569
1201	16	0.441902514191014
1201	17	0.5818405803954647
1201	18	0.6775027172365142
1201	19	0.46226682060319035
1201	20	0.3971166382675406
1201	21	0.2841030156566049
1201	22	0.8470044345241945
1201	23	0.6650261901983328
1201	24	0.5906748284781216
1201	25	1.0086914450182363
1202	1	-0.0028271016251224523
1202	2	0.18544489559728916
1202	3	0.34872995127515694
1202	4	0.057196489804354655
1202	5	-0.4322390149391637
1202	6	-0.51912889736316
1202	7	0.31379514294336985
1202	8	-0.6313744047331937
1202	9	-0.32179541050491167
1202	10	-5.127352274740815E-4
1202	11	0.6830482669076119
1202	12	0.19974165689521328
1202	13	0.13396285198153635
1202	14	-0.1280699044957032
1202	15	-0.07999005901883294
1202	16	0.33434601701586786
1202	17	-0.5286820184745906
1202	18	0.3381665590444243
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2210	13	-0.04977555543771217
2210	14	0.16676540090883663
2210	15	0.13531214756894627
2210	16	0.05660377205264666
2210	17	-0.1878921240070497
2210	18	-0.34506017584524784
2210	19	-0.19119229889366807
2210	20	-0.02678273280164234
2210	21	0.1061942106251621
2210	22	0.10573084961853851
2210	23	0.8156551210159435
2210	24	0.2507377215598794
2210	25	0.003502765772950056
2211	1	-0.11684867327381454
2211	2	-0.2863065168320915
2211	3	-0.4125694837530922
2211	4	-0.26515841517766603
2211	5	-0.505492738616617
2211	6	0.08506986997119981
2211	7	0.23912898988739073
2211	8	-0.08791010067567129
2211	9	-0.0626146195444548
2211	10	-0.18123949434406228
2211	11	0.031468914057072084
2211	12	-0.05873291937597003
2211	13	-0.6892266703605259
2211	14	0.09785459473388158
2211	15	-0.28485652024122743
2211	16	-0.3751429403544435
2211	17	-0.545053358998473
2211	18	-0.8092397070059079
2211	19	-0.6316725733362034
2211	20	-0.4968227556718574
2211	21	-0.3783969843433894
2211	22	-0.29579859577883383
2211	23	-0.5645192643471262
2211	24	-0.013870626067337355
2211	25	0.026115687442477054
2212	1	0.5147836349384214
2212	2	0.3273861721394091
2212	3	0.16332890534232192
2212	4	0.2569633601968988
2212	5	0.31016852081787505
2212	6	0.4364977172168878
2212	7	0.21123386766141294
2212	8	0.7099241901883602
2212	9	-0.13711895659525908
2212	10	0.3857735731336902
2212	11	0.30618165484787596
2212	12	0.4607838092485679
2212	13	0.20026302210314384
2212	14	0.47074239466628853
2212	15	0.24199658328473816
2212	16	-0.010591290022119892
2212	17	0.21791554941653146
2212	18	0.34331746320925305
2212	19	0.27775628776551997
2212	20	-0.05517580287248336
2212	21	0.34310239607792425
2212	22	-0.3365922197508979
2212	23	0.2709121877200147
2212	24	0.20771324235668231
2212	25	0.11547583163533659
2213	1	-0.2311977388423898
2213	2	-0.9672008257751799
2213	3	-0.358852875158437
2213	4	-0.4551462728484523
2213	5	-0.37901454201647056
2213	6	-0.06804226179946937
2213	7	-0.06093172175869199
2213	8	-0.0578050431315873
2213	9	-0.5566622385920752
2213	10	-1.2376089379086253
2213	11	-0.8628532272162914
2213	12	-0.8418339646721407
2213	13	-0.3845069997871988
2213	14	-0.302802674825827
2213	15	-0.5638529303352016
2213	16	-0.18874498871258538
2213	17	-1.0680577935882738
2213	18	-0.8783190016949618
2213	19	-0.17966117010529103
2213	20	-0.39614700865464414
2213	21	-0.2788034884084354
2213	22	-0.9288230916082085
2213	23	-1.5047182929607885
2213	24	-1.8822055431827422
2213	25	-2.279897405155978
2214	1	0.4534587171774618
2214	2	0.28863353112954115
2214	3	0.5448645690047016
2214	4	0.34082043810334284
2214	5	0.2979463283116601
2214	6	0.524981576969239
2214	7	0.3117527090938381
2214	8	0.47730136651421873
2214	9	-0.08749471546772014
2214	10	0.511833320131629
2214	11	0.32324458442986526
2214	12	-0.024560257505463312
2214	13	0.20776482735039536
2214	14	0.36641247570182855
2214	15	0.5994087947794853
2214	16	0.20320347342362766
2214	17	0.3544724748798771
2214	18	0.32783245344754874
2214	19	-0.15619840721455347
2214	20	0.1501142401860065
2214	21	0.4051761571434156
2214	22	-0.04876894677076393
2214	23	-0.7967244093220529
2214	24	-0.28114771348830914
2214	25	-0.7037186269242142
2215	1	-0.9599415234841011
2215	2	-0.5529138715177382
2215	3	-1.7224745845834626
2215	4	-1.6419108146168995
2215	5	-0.6015646678735962
2215	6	-0.028040773833396315
2215	7	-0.3762193332011492
2215	8	-0.47118681802712103
2215	9	-0.4631187713831295
2215	10	-1.7350111971371192
2215	11	-0.6772418591554796
2215	12	-0.685957118664227
2215	13	-1.0994498990890946
2215	14	-1.1105326585822013
2215	15	-1.7622236074837616
2215	16	-1.1864634407318846
2215	17	-0.9329132748832976
2215	18	-0.8194575437361991
2215	19	-1.5093820524691708
2215	20	-1.712017406809565
2215	21	-1.2535452642959442
2215	22	-0.7801752807782947
2215	23	-1.5656381039890945
2215	24	-1.7218399995171367
2215	25	-1.4182658503554322
2216	1	-0.30677770335154264
2216	2	-0.21536475722862036
2216	3	-1.1675261132450387
2216	4	-0.6575347810670422
2216	5	0.17633952453535073
2216	6	0.05359808896410101
2216	7	-0.1791007048292954
2216	8	-0.3718601964287416
2216	9	-0.2374907038071612
2216	10	-0.08262791385515555
2216	11	-0.12132763883241893
2216	12	-0.19349128368823898
2216	13	-1.4205548217630266
2216	14	-0.3359490551478359
2216	15	-0.5275201453397997
2216	16	-0.4529704721782579
2216	17	-1.3917498780927602
2216	18	-0.872295919690373
2216	19	-0.5698273553571553
2216	20	-0.4455103753631917
2216	21	0.09031429198870455
2216	22	0.2805081519963153
2216	23	0.014406611511837752
2216	24	-0.1119893598738031
2216	25	-0.4532974025654113
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	213.0
3	20.0
4	27.0
5	27.0
6	14.0
7	12.0
8	40.0
9	85.0
10	170.0
11	291.0
12	423.0
13	615.0
14	804.0
15	1057.0
16	1109.0
17	1230.0
18	1239.0
19	1291.0
20	1345.0
21	1249.0
22	1298.0
23	1297.0
24	1265.0
25	1298.0
26	1261.0
27	1368.0
28	1462.0
29	1719.0
30	2028.0
31	2581.0
32	3453.0
33	4995.0
34	7339.0
35	11915.0
36	23735.0
37	86256.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.92659800854873	24.46997274406863	15.363930707739588	22.23949853964305
2	14.580352086517559	23.81607711559423	40.1818281462176	21.421742651670613
3	16.813340073204746	27.976759624110066	32.577330889114904	22.632569413570284
4	11.758004776061211	21.18707052000585	39.74913494809689	27.305789755836052
5	11.472582915430223	40.435553551516335	36.26647492885999	11.825388604193451
6	27.221464412724707	41.870463292249234	18.27592926389633	12.632143031129731
7	22.362075264888563	36.34697357203751	24.718061137498477	16.572890025575447
8	21.14344531878091	39.395083684556724	22.585085389918873	16.876385606743487
9	21.61520190023753	20.347158779462816	24.37968207564407	33.657957244655584
10	12.904994243736637	33.156076969464394	35.989133149376556	17.94979563742241
11	28.477821352885297	28.22875307529291	25.889094585048593	17.404330986773196
12	17.701400010961375	30.3739655203912	33.15084676609038	18.773787702557048
13	23.57312089264071	27.885277153489618	28.220870105428563	20.320731848441106
14	17.791242920650387	28.349674197673714	29.822787893550938	24.03629498812496
15	19.711731680286444	35.49524424254972	25.93805945610211	18.854964621061736
16	18.49443114902842	34.77167406541345	28.176741750245103	18.557153035313032
17	17.300993813026746	33.63375066984947	30.710771179422224	18.354484337701564
18	17.49768597457008	32.71057631412286	31.75086471476592	18.04087299654114
19	18.008708096093535	32.418475778704746	32.10059982340225	17.47221630179947
20	19.01519949335022	32.0608223315633	31.154089735470357	17.769888439616118
21	18.913306905853624	31.883475518045586	31.01818868246226	18.185028893638528
22	18.773293060190486	31.702311645921128	31.151194796969772	18.373200496918617
23	18.768307015887903	31.8866350410747	31.02251344290647	18.322544500130928
24	19.250470511563318	31.39119153627355	30.986155692124033	18.372182260039104
25	18.89338895825086	31.74030877919547	30.89131824964219	18.474984012911477
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1575.0
1	1575.0
2	1092.0
3	609.0
4	609.0
5	609.0
6	714.5
7	820.0
8	820.0
9	820.0
10	1098.0
11	1376.0
12	1376.0
13	1376.0
14	2260.0
15	3144.0
16	3144.0
17	3144.0
18	4675.5
19	6207.0
20	6207.0
21	6207.0
22	8515.5
23	10824.0
24	10824.0
25	10824.0
26	13299.5
27	15775.0
28	15775.0
29	15775.0
30	17803.5
31	19832.0
32	19832.0
33	19832.0
34	20612.5
35	21393.0
36	21393.0
37	21393.0
38	20421.0
39	19449.0
40	19449.0
41	19449.0
42	17966.5
43	16484.0
44	16484.0
45	16484.0
46	15107.0
47	13730.0
48	13730.0
49	13730.0
50	12475.5
51	11221.0
52	11221.0
53	11221.0
54	9806.5
55	8392.0
56	8392.0
57	8392.0
58	7208.0
59	6024.0
60	6024.0
61	6024.0
62	5018.5
63	4013.0
64	4013.0
65	4013.0
66	3066.5
67	2120.0
68	2120.0
69	2120.0
70	1525.5
71	931.0
72	931.0
73	931.0
74	644.5
75	358.0
76	358.0
77	358.0
78	263.0
79	168.0
80	168.0
81	168.0
82	113.0
83	58.0
84	58.0
85	58.0
86	40.0
87	22.0
88	22.0
89	22.0
90	13.0
91	4.0
92	4.0
93	4.0
94	2.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32212774492344903
2	0.1884143413703193
3	0.20300125812156977
4	0.23035172703016454
5	0.2540554667509466
6	0.19145328236016312
7	0.18902212956828804
8	0.20847135190328875
9	0.2072557755073512
10	0.2200193276646954
11	0.1938844351520382
12	0.19327664695406946
13	0.2090791401012575
14	0.19510001154797577
15	0.1878065531723505
16	0.19084549416219435
17	0.19145328236016312
18	0.19145328236016312
19	0.1920610705581319
20	0.19145328236016312
21	0.18719876497438173
22	0.1938844351520382
23	0.19327664695406946
24	0.2115102928931326
25	0.20421683451750736
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	164531.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.75814143416737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.77691690724076	52.022611946801355
2	14.328598269520738	19.417583761991043
3	4.660569077116591	9.473744960729187
4	1.858328106270658	5.036674346231027
5	0.8742433096065291	2.9618550910096886
6	0.5097480855628104	2.0723749726416574
7	0.2996699790100224	1.4213556574934545
8	0.19911556875163086	1.0793360695374146
9	0.13838523769436614	0.8439053857286158
>10	0.3414829565702623	3.2784809650325424
>50	0.006969039891473915	0.32447517241644563
>100	0.003982308509413665	0.8702140798694696
>500	9.955771273534163E-4	0.3723706760371684
>1k	9.955771273534163E-4	0.8250169144809002
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	1223	0.743324966115808	No Hit
GGTATCAACGCAGAGTACTTTTTTT	552	0.33549908527876204	No Hit
GTATCAACGCAGAGTACTTTTTTTT	495	0.30085515799454204	No Hit
TATCAACGCAGAGTACTTTTTTTTT	370	0.22488163324844557	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	232	0.14100686192875508	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	193	0.11730312220797295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACATC	25	0.0060129208	18.998169	8
CTCCAGG	35	0.0021341462	16.314022	1
TATTCTG	35	0.0021385138	16.309034	5
ATGTACT	45	6.6441944E-4	14.79894	4
CCATGTA	45	6.6750916E-4	14.789897	2
GTCTTAG	40	0.005192238	14.274772	1
GTGCAGA	60	3.9983194E-4	12.688685	1
TTAATAT	75	2.0367198E-4	11.409349	3
GGTATCA	150	2.3064786E-8	10.150948	1
ATCAACG	260	1.9274812E-6	6.586342	4
TCAACGC	275	6.8863847E-7	6.5730352	5
GTATCAA	240	2.8144723E-5	6.3443427	1
CAACGCA	270	3.6680885E-6	6.332723	6
AACGCAG	290	1.1704105E-5	5.895983	7
TATCAAC	265	1.2033665E-4	5.7405534	3
CGCAGAG	300	2.0194026E-5	5.6994505	9
ACGCAGA	305	2.6314323E-5	5.6060166	8
>>END_MODULE