FastQCFastQC Report
Wed 25 May 2016
SRR1295054_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295054_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164531
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTT18451.1213692252523841No Hit
GTATCAACGCAGAGTACTTTTTTTT16731.0168296552017553No Hit
TATCAACGCAGAGTACTTTTTTTTT12060.7329925667503389No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8640.5251290030450189No Hit
GTGGTATCAACGCAGAGTACTTTTT3310.2011778935276635No Hit
GTACTTTTTTTTTTTTTTTTTTTTT2880.1750430010150063No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2840.1726118482231312No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2070.12581215697953577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2800.012.58256151
GTGGTAT1053.3116048E-610.8822141
TGGTATC1053.4098139E-610.8557542
GTACTGT750.002628906910.1320366
GTATCAA4900.08.5503111
ATCAACG6000.06.6491493
TCAACGC6000.06.6491494
CAACGCA6150.06.48697475
AACGCAG6350.06.4322486
TATCAAC6250.06.3831832
AGTACTT6801.8189894E-125.30814413
CTTTTTT7700.05.18115517
ACGCAGA7550.05.158287
GTACTTT7201.8189894E-125.14517514
TACTTTT7551.8189894E-125.032468315
Produced by FastQC (version 0.11.5)