##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295054_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 164531 Sequences flagged as poor quality 0 Sequence length 25 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.091192541223236 33.0 33.0 33.0 27.0 33.0 2 32.07900638785396 33.0 33.0 33.0 27.0 33.0 3 32.15775750466477 33.0 33.0 33.0 27.0 33.0 4 35.93218299287064 37.0 37.0 37.0 33.0 37.0 5 36.005883389756335 37.0 37.0 37.0 33.0 37.0 6 35.45863089630526 37.0 37.0 37.0 33.0 37.0 7 35.28784241267603 37.0 37.0 37.0 33.0 37.0 8 35.317192504755944 37.0 37.0 37.0 33.0 37.0 9 35.23257623183473 37.0 37.0 37.0 33.0 37.0 10 35.266703539150676 37.0 37.0 37.0 33.0 37.0 11 35.24853674991339 37.0 37.0 37.0 33.0 37.0 12 35.247473120566944 37.0 37.0 37.0 33.0 37.0 13 35.19053552218123 37.0 37.0 37.0 33.0 37.0 14 36.8364441959266 40.0 37.0 40.0 33.0 40.0 15 36.907628349672706 40.0 37.0 40.0 33.0 40.0 16 36.9656782004607 40.0 37.0 40.0 33.0 40.0 17 36.93221946016252 40.0 37.0 40.0 33.0 40.0 18 36.949176750885854 40.0 37.0 40.0 33.0 40.0 19 36.978180403692924 40.0 37.0 40.0 33.0 40.0 20 36.9081085023491 40.0 37.0 40.0 33.0 40.0 21 36.94848995022215 40.0 37.0 40.0 33.0 40.0 22 36.899307729242516 40.0 37.0 40.0 33.0 40.0 23 36.87044386772098 40.0 37.0 40.0 33.0 40.0 24 36.88305547282883 40.0 37.0 40.0 33.0 40.0 25 35.90296053631231 37.0 37.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21605085797453683 1101 2 0.2817903643696482 1101 3 0.07513549022989707 1101 4 -0.12938910357890165 1101 5 0.05936802581521761 1101 6 0.0702749299205152 1101 7 0.2033031678610584 1101 8 0.10119404666111365 1101 9 0.08396029306388897 1101 10 0.1741328338195416 1101 11 0.0929354374014224 1101 12 -0.13801974773897996 1101 13 0.18941083284057925 1101 14 0.23764713992555642 1101 15 -0.1728913991640013 1101 16 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0.1554977344727959 2215 6 -0.3155285874522349 2215 7 -0.33077045774990665 2215 8 -0.1873870433995819 2215 9 -0.38677994999218157 2215 10 -0.4086258377889749 2215 11 -0.3297251848575513 2215 12 -0.4386059765306456 2215 13 0.04495047342474123 2215 14 -0.5540570581813853 2215 15 -0.6498778583125642 2215 16 -0.5648131827989502 2215 17 -0.8247602145635042 2215 18 -0.7714434708705582 2215 19 -1.0417966723302712 2215 20 -0.7700009308697915 2215 21 -0.42173293447207527 2215 22 -0.18629470050647257 2215 23 -0.671804819059787 2215 24 -0.5323518055414098 2215 25 -0.12218100935226772 2216 1 -0.3260676383774026 2216 2 -0.3561957221039975 2216 3 -0.2684915902433467 2216 4 -0.02246164827339925 2216 5 -0.7885549716087752 2216 6 -0.29399106215093695 2216 7 -0.2556272490819751 2216 8 -0.0016457318330793669 2216 9 -0.19594530473955984 2216 10 -0.3356390275388037 2216 11 -0.4499543187262418 2216 12 -0.7213852168963015 2216 13 -0.008787504323343853 2216 14 -0.5996324725935338 2216 15 -0.46071012226632035 2216 16 -0.3984000803634089 2216 17 -0.5716281450824994 2216 18 -0.6717464250040948 2216 19 -0.5264968271146984 2216 20 -0.537903668040542 2216 21 -0.26780842164613716 2216 22 -0.253328263591051 2216 23 -0.33212576522920045 2216 24 -0.3214216437453814 2216 25 -0.759165226632561 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 4.0 9 21.0 10 19.0 11 51.0 12 87.0 13 165.0 14 252.0 15 329.0 16 380.0 17 479.0 18 546.0 19 658.0 20 687.0 21 760.0 22 788.0 23 875.0 24 926.0 25 971.0 26 973.0 27 1203.0 28 1224.0 29 1522.0 30 1868.0 31 2513.0 32 3433.0 33 5165.0 34 7432.0 35 12240.0 36 24161.0 37 94797.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.41874280983888 23.64033672779952 14.684058994320914 23.256861468040686 2 14.543597972356952 22.907625542467454 40.15049293120844 22.398283553967154 3 16.560405030055126 27.017400976107847 33.858057144246374 22.564136849590653 4 11.434927156584473 19.97313576164978 40.77772577812084 27.814211303644903 5 11.010757916489395 39.694888470187806 37.3214611317085 11.972892481614297 6 27.45500847864536 41.73924670730744 17.832505728403767 12.973239085643435 7 22.41765989388018 36.219314293355055 24.678024202126043 16.685001610638725 8 21.81074461037264 38.49777242917662 22.095192944708835 17.596290015741907 9 22.21344305936267 18.268289866347377 24.64459585123776 34.87367122305219 10 13.699545982216119 31.89550905300521 35.86315040934535 18.54179455543332 11 29.125818234861516 26.66062930390018 25.832821778266705 18.3807306829716 12 18.574007329925667 29.2467680862573 33.63986118117558 18.53936340264145 13 24.76448064182824 25.643347717741445 29.118093964626514 20.474077675803805 14 18.512620721930823 26.715330241717368 30.837349800341578 23.934699236010236 15 20.1712747141876 33.50857893041433 27.567449295269586 18.752697060128487 16 18.149771167743463 33.3262424710237 29.68316001239888 18.840826348833957 17 17.15786082865843 32.13133087381709 31.698585676863328 19.012222620661152 18 17.258145881323276 30.385155381052808 33.86413502622606 18.492563711397853 19 17.482419726373756 30.250834189301713 34.95876157076782 17.307984513556715 20 18.400179905306597 30.16452826519015 34.04890263840857 17.386389191094686 21 18.657274313047388 29.69713914095216 33.84954810947481 17.79603843652564 22 18.263427560763628 29.688022317982632 33.81368860579465 18.23486151545909 23 18.483446888428322 29.77311266569826 33.777829102114495 17.965611343758926 24 18.380954117511198 29.696892341167818 33.67856122628837 18.243592315032608 25 18.272766503169656 29.935756006539883 33.679169021874564 18.112308468415904 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 178.0 1 178.0 2 188.5 3 199.0 4 199.0 5 199.0 6 428.5 7 658.0 8 658.0 9 658.0 10 947.5 11 1237.0 12 1237.0 13 1237.0 14 1875.5 15 2514.0 16 2514.0 17 2514.0 18 4174.5 19 5835.0 20 5835.0 21 5835.0 22 8416.0 23 10997.0 24 10997.0 25 10997.0 26 13662.0 27 16327.0 28 16327.0 29 16327.0 30 18513.5 31 20700.0 32 20700.0 33 20700.0 34 21819.5 35 22939.0 36 22939.0 37 22939.0 38 21535.5 39 20132.0 40 20132.0 41 20132.0 42 18268.0 43 16404.0 44 16404.0 45 16404.0 46 15115.5 47 13827.0 48 13827.0 49 13827.0 50 12551.5 51 11276.0 52 11276.0 53 11276.0 54 9840.5 55 8405.0 56 8405.0 57 8405.0 58 7149.5 59 5894.0 60 5894.0 61 5894.0 62 4843.0 63 3792.0 64 3792.0 65 3792.0 66 2831.0 67 1870.0 68 1870.0 69 1870.0 70 1375.0 71 880.0 72 880.0 73 880.0 74 598.0 75 316.0 76 316.0 77 316.0 78 216.0 79 116.0 80 116.0 81 116.0 82 72.5 83 29.0 84 29.0 85 29.0 86 17.5 87 6.0 88 6.0 89 6.0 90 3.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1483003203043803 2 0.003038940989843859 3 0.0 4 0.0 5 6.077881979687719E-4 6 0.0 7 0.0 8 0.0012155763959375437 9 0.0 10 0.0 11 0.0 12 0.0 13 6.077881979687719E-4 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0012155763959375437 25 0.0012155763959375437 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 164531.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.1369543204497 #Duplication Level Percentage of deduplicated Percentage of total 1 76.2213485929673 49.64826501543184 2 14.52750674376701 18.925550863175534 3 4.632952251225592 9.053291974707179 4 2.059860462653151 5.366921474493547 5 0.9329479052507231 3.03846925438378 6 0.5188468114394799 2.027766063362663 7 0.31928945697002586 1.4558279941560435 8 0.2045447394045712 1.0658737077667073 9 0.14667353884963089 0.85984808400604 >10 0.42006503074884827 3.7773522576093974 >50 0.0069844541916033445 0.32561140412785994 >100 0.0049888958511452456 0.8234523932734503 >500 9.977791702290492E-4 0.5615334394540339 >1k 0.0029933375106871475 3.070236074051917 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTT 1845 1.1213692252523841 No Hit GTATCAACGCAGAGTACTTTTTTTT 1673 1.0168296552017553 No Hit TATCAACGCAGAGTACTTTTTTTTT 1206 0.7329925667503389 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 864 0.5251290030450189 No Hit GTGGTATCAACGCAGAGTACTTTTT 331 0.2011778935276635 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 288 0.1750430010150063 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 284 0.1726118482231312 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 207 0.12581215697953577 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 280 0.0 12.5825615 1 GTGGTAT 105 3.3116048E-6 10.882214 1 TGGTATC 105 3.4098139E-6 10.855754 2 GTACTGT 75 0.0026289069 10.132036 6 GTATCAA 490 0.0 8.550311 1 ATCAACG 600 0.0 6.649149 3 TCAACGC 600 0.0 6.649149 4 CAACGCA 615 0.0 6.4869747 5 AACGCAG 635 0.0 6.432248 6 TATCAAC 625 0.0 6.383183 2 AGTACTT 680 1.8189894E-12 5.308144 13 CTTTTTT 770 0.0 5.181155 17 ACGCAGA 755 0.0 5.15828 7 GTACTTT 720 1.8189894E-12 5.145175 14 TACTTTT 755 1.8189894E-12 5.0324683 15 >>END_MODULE