FastQCFastQC Report
Wed 25 May 2016
SRR1295053_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295053_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3889776
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC383180.9850952856925437No Hit
CCCATGTACTCTGCGTTGATACCAC360410.9265572104923266No Hit
ATTCCATTCCATTCCATTCCATTCC184410.47408899638436763No Hit
GAGTACATGGGAAGCAGTGGTATCA174220.44789211512436705No Hit
GAATGGAATGGAATGGAATGGAATG136490.35089424172497335No Hit
CATGTACTCTGCGTTGATACCACTG131210.3373201953017346No Hit
GCGTTGATACCACTGCTTCCCATGT117020.3008399455392804No Hit
GTATCAACGCAGAGTACATGGGAAG99440.25564454097099676No Hit
ACGCAGAGTACATGGGAAGCAGTGG94780.24366441666563832No Hit
GGTATCAACGCAGAGTACATGGGAA89400.2298332860298382No Hit
GCTTCCCATGTACTCTGCGTTGATA88230.22682540074287055No Hit
TATCAACGCAGAGTACATGGGAAGC86820.22320051334575564No Hit
ACTCTGCGTTGATACCACTGCTTCC79860.20530745215148635No Hit
GTATCAACGCAGAGTACTTTTTTTT57120.14684650221503756No Hit
GTGGTATCAACGCAGAGTACATGGG56480.145201163254645No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN47870.1230662125531136No Hit
CAGTGGTATCAACGCAGAGTACATG44510.11442818301105256No Hit
GGTATCAACGCAGAGTACTTTTTTT44100.11337413773955106No Hit
ACATGGGAAGCAGTGGTATCAACGC40440.10396485555980602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC901.3278623E-1015.853111
TCGTACC901.3460522E-1015.8400282
CGTACCC954.8803486E-914.0059193
TACGACC1600.013.0697094
GTACGAC1501.8189894E-1212.6720223
CCGTTCG550.003072006912.0897869
AGCGTAA550.003072303512.08962910
ATAGCCG550.003076457412.08744816
TCGGAAC2100.010.85403513
GTGCTAG3600.010.8329591
TATTCCG5400.010.7369895
GGTACGA1605.4205884E-1010.6920192
GGGTACG1803.45608E-1110.5687411
ACGACCA1803.45608E-1110.5609725
GTCCTAC1553.594323E-910.432371
ACTCGGA2500.010.25679911
GTCTAGC2950.09.9955211
CTCGGAA2500.09.87729912
CGACCAT1752.582965E-99.7701436
GGAACGA5000.09.68710215
Produced by FastQC (version 0.11.5)