##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295053_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3889776
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3152178942952	33.0	33.0	33.0	27.0	33.0
2	31.23801550526303	33.0	33.0	33.0	27.0	33.0
3	31.251759227266557	33.0	33.0	33.0	27.0	33.0
4	34.85454946505917	37.0	37.0	37.0	33.0	37.0
5	35.03371428071951	37.0	37.0	37.0	33.0	37.0
6	35.13009463784033	37.0	37.0	37.0	33.0	37.0
7	34.98048448034026	37.0	37.0	37.0	33.0	37.0
8	34.894382864206065	37.0	37.0	37.0	33.0	37.0
9	34.90087372640481	37.0	37.0	37.0	33.0	37.0
10	34.783272352958114	37.0	37.0	37.0	33.0	37.0
11	34.98618378024853	37.0	37.0	37.0	33.0	37.0
12	34.88923243909161	37.0	37.0	37.0	33.0	37.0
13	34.827237866653505	37.0	37.0	37.0	33.0	37.0
14	36.263480981938294	40.0	37.0	40.0	27.0	40.0
15	36.41888273257894	40.0	37.0	40.0	33.0	40.0
16	36.478805977516444	40.0	37.0	40.0	33.0	40.0
17	36.400966533805544	40.0	37.0	40.0	33.0	40.0
18	36.33984270559539	40.0	37.0	40.0	27.0	40.0
19	36.44695324358009	40.0	37.0	40.0	33.0	40.0
20	36.47233028328623	40.0	37.0	40.0	33.0	40.0
21	36.468583537972364	40.0	37.0	40.0	33.0	40.0
22	36.36248642595358	40.0	37.0	40.0	27.0	40.0
23	36.27543976825401	40.0	37.0	40.0	27.0	40.0
24	36.35295939920448	40.0	37.0	40.0	27.0	40.0
25	35.31823297794012	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.28689258179144517
1101	2	0.25207481018572153
1101	3	0.24750260670393942
1101	4	0.2906144219615783
1101	5	0.2031881273772953
1101	6	0.18028590682990142
1101	7	0.2861046923103885
1101	8	0.33958666999686926
1101	9	0.3353419625511478
1101	10	0.5897547252708009
1101	11	0.36577188437530594
1101	12	0.41409065811605217
1101	13	0.41194040794003683
1101	14	0.8070918217738097
1101	15	0.7297363474582781
1101	16	0.6143611395401649
1101	17	0.6888432206079003
1101	18	0.6817028885564085
1101	19	0.532507769723594
1101	20	0.5402267012794582
1101	21	0.5473287526472532
1101	22	0.5900960018205481
1101	23	0.6709933791674416
1101	24	0.6248643834726906
1101	25	0.707598478675024
1102	1	0.2260424939986052
1102	2	0.2383010889820376
1102	3	0.26631737699252156
1102	4	0.3117060059764256
1102	5	0.0314010026060032
1102	6	0.037074639818385435
1102	7	0.08383125320381879
1102	8	0.22801161898210864
1102	9	0.2286423933424402
1102	10	0.3403568312444065
1102	11	0.13870152882748243
1102	12	0.3039021539689557
1102	13	0.2309428914492173
1102	14	0.5315174245916197
1102	15	0.4507109798092799
1102	16	0.35786199110435746
1102	17	0.46023530396100654
1102	18	0.45964342142995207
1102	19	0.35293160086606434
1102	20	0.38915151580719964
1102	21	0.36214544225079237
1102	22	0.4404453276875344
1102	23	0.5034262163262184
1102	24	0.42649748849191127
1102	25	0.46332726470831886
1103	1	0.17269132803928855
1103	2	0.14095424635538123
1103	3	0.19943927153609664
1103	4	0.18268423857638538
1103	5	0.06767867088184687
1103	6	0.06507644748402441
1103	7	0.0629364575399265
1103	8	0.2472639395448084
1103	9	0.28132709790653365
1103	10	0.32255708864551025
1103	11	0.2021726786960727
1103	12	0.3198424087572107
1103	13	0.2858250443234098
1103	14	0.568527007669573
1103	15	0.5257054546075253
1103	16	0.42571934180838156
1103	17	0.4315643027785825
1103	18	0.5567059765258975
1103	19	0.48559372929275924
1103	20	0.4736937272469035
1103	21	0.4326955778418693
1103	22	0.43603724050195325
1103	23	0.5226446644570828
1103	24	0.5296553480374158
1103	25	0.6005240392419822
1104	1	0.22520551885438067
1104	2	0.18833438394529622
1104	3	0.15689051046684455
1104	4	0.14855169302384752
1104	5	-9.317111175448645E-4
1104	6	0.019109999403994493
1104	7	0.06938328871398625
1104	8	0.26818182484202424
1104	9	0.2438420041761873
1104	10	0.32434555111162666
1104	11	0.2642013715547904
1104	12	0.20524679364718423
1104	13	0.27356253581216805
1104	14	0.5545198104518008
1104	15	0.5235598156567463
1104	16	0.2937783889574206
1104	17	0.40131179370146697
1104	18	0.5015101646136841
1104	19	0.39635433982016366
1104	20	0.4467601678129256
1104	21	0.40647793679644195
1104	22	0.42179659289613625
1104	23	0.47589805782212125
1104	24	0.3971901826734907
1104	25	0.553914870616417
1105	1	0.2221694275951407
1105	2	0.18392646973516236
1105	3	0.16032873590818042
1105	4	0.19604200922552195
1105	5	0.08277331151948175
1105	6	0.12252169126117707
1105	7	0.21064414066484716
1105	8	0.2279508959792551
1105	9	0.14881398829489711
1105	10	0.2744978414352275
1105	11	0.1843664650163035
1105	12	0.26772698508457893
1105	13	0.1779714157260841
1105	14	0.43653529940973357
1105	15	0.3569932195601382
1105	16	0.26890046322399286
1105	17	0.22152418919603178
1105	18	0.17928581836024193
1105	19	0.21133392972881637
1105	20	0.29483890594756446
1105	21	0.2675666828801013
1105	22	0.28394071941589516
1105	23	0.3951353775496571
1105	24	0.32587513961473746
1105	25	0.2635253278154934
1106	1	0.02841768641904352
1106	2	-0.1641811406962681
1106	3	-0.1673354453640279
1106	4	-0.11378605290980914
1106	5	-0.4155285207022388
1106	6	-0.2963046125307045
1106	7	-0.7451284143797778
1106	8	-0.24081073499887395
1106	9	0.03938580421271354
1106	10	-0.3253507417460213
1106	11	-0.46766961410774144
1106	12	-0.7631102388632414
1106	13	-0.31335560721747413
1106	14	-0.4724582495768104
1106	15	-0.3047786481647563
1106	16	-0.19519049073168304
1106	17	-0.25242765994276084
1106	18	-0.44524126608857273
1106	19	-0.5425267064189114
1106	20	-0.1201774190692575
1106	21	0.012825029223861861
1106	22	0.20257936333437954
1106	23	-0.4896652050922512
1106	24	-0.12863275650644823
1106	25	0.13790054964191967
1107	1	-1.223579998812454
1107	2	-1.3095988630440907
1107	3	-0.9680185082065371
1107	4	-0.9683647232092909
1107	5	-1.1105511318314356
1107	6	-1.0875234975980206
1107	7	-1.2114826054870207
1107	8	-1.039227955954992
1107	9	-1.1784397623993286
1107	10	-1.0489937860121827
1107	11	-1.0085741744684569
1107	12	-1.0476665253876902
1107	13	-0.935247387849202
1107	14	-1.2790447905742255
1107	15	-1.207768719153819
1107	16	-1.2060304036944487
1107	17	-1.244695648052442
1107	18	-1.2815820099550166
1107	19	-1.3399028575130316
1107	20	-1.437240106371199
1107	21	-1.3839759461558003
1107	22	-1.3600350337011662
1107	23	-1.0978153061284388
1107	24	-1.064537344149656
1107	25	-1.0225859943262563
1108	1	-0.11425511352811668
1108	2	-0.23205584552438552
1108	3	-0.1460283686417263
1108	4	-0.28600995222400627
1108	5	-0.5740703638514333
1108	6	-0.11796374558201705
1108	7	-0.004749698937665414
1108	8	0.050301194710428376
1108	9	-0.3340568607411001
1108	10	-0.47718560596141657
1108	11	-0.18371397621952923
1108	12	0.03849959211692067
1108	13	0.058245832392366026
1108	14	-0.32260805747959864
1108	15	-0.019052799064908754
1108	16	-0.8874923726061894
1108	17	-0.8041968294364708
1108	18	-0.6817077766305388
1108	19	-0.14257458111487864
1108	20	-1.0494757134445578
1108	21	-0.2421931033172342
1108	22	-0.36387315272470033
1108	23	-0.24530167479444032
1108	24	-0.5831294981457731
1108	25	-1.1875628042731066
1109	1	0.17067765487211162
1109	2	0.12711390211127238
1109	3	0.23181704641882916
1109	4	0.22762616972919858
1109	5	0.20260484660745703
1109	6	0.11389209693013669
1109	7	0.20115659441279377
1109	8	0.23292206409777805
1109	9	0.2829863241935726
1109	10	0.2868514936252069
1109	11	0.27705586789473813
1109	12	0.22956432136713545
1109	13	0.279309211539136
1109	14	0.6382309389019909
1109	15	0.5043799112494725
1109	16	0.4559705711721165
1109	17	0.49111482699014886
1109	18	0.4536487404958294
1109	19	0.4142034194153865
1109	20	0.4031734875263382
1109	21	0.3981023063156286
1109	22	0.4364280443893236
1109	23	0.49729696086503594
1109	24	0.5351619276782458
1109	25	0.39148979267891804
1110	1	0.07560348912043224
1110	2	0.03471781791154882
1110	3	0.07593168827073882
1110	4	-0.9112894384941015
1110	5	-0.294011848857231
1110	6	0.02275669680660286
1110	7	0.05866073068753508
1110	8	0.09877077663009715
1110	9	0.11497240940354914
1110	10	-0.002568370469241188
1110	11	-0.021034506898665484
1110	12	0.14603846421434952
1110	13	0.13837708216658484
1110	14	0.24094516830108148
1110	15	0.1590692546520458
1110	16	0.1359888287778901
1110	17	0.23620687131276696
1110	18	0.3277665267639165
1110	19	0.15476495724344375
1110	20	0.08833707962173776
1110	21	0.1918882001409159
1110	22	-0.33244739578201177
1110	23	0.03876649262915066
1110	24	0.20765236060046988
1110	25	0.3304673403701983
1111	1	-0.02427707604912399
1111	2	-0.20490092148765626
1111	3	-0.16478823845768886
1111	4	-0.07198780288762663
1111	5	-0.078083762201409
1111	6	0.0257715626974786
1111	7	0.018764260622411655
1111	8	0.1851917523747062
1111	9	0.09368533994608441
1111	10	0.09441767742579543
1111	11	-0.07746020922598262
1111	12	0.09980009402281098
1111	13	0.03562810789126303
1111	14	-0.12676192897363592
1111	15	0.04772888536806619
1111	16	-0.0316775046079627
1111	17	0.10195665925920849
1111	18	0.04962402958385326
1111	19	-0.09313820534949713
1111	20	-0.005447077519718846
1111	21	-0.049689304619050745
1111	22	-0.04947277864575028
1111	23	0.1992131140262572
1111	24	0.13095356481967002
1111	25	0.16808073141061897
1112	1	-0.05140012836373131
1112	2	-0.35388615021649983
1112	3	-0.12862386472339082
1112	4	-0.22216676692276138
1112	5	-0.0800069010899449
1112	6	-0.09229094643463043
1112	7	-0.0689461021673381
1112	8	0.02141833085431699
1112	9	-0.11627928471479976
1112	10	0.16728847455240015
1112	11	0.019933889196337873
1112	12	0.026892768453976146
1112	13	0.0015201182647359701
1112	14	-0.24132819501296865
1112	15	-0.2200276866610622
1112	16	-0.16222270959382712
1112	17	-0.34645879669984936
1112	18	-0.2264353915016386
1112	19	-0.11785215370223057
1112	20	-0.9384359795608717
1112	21	-1.3059994536895445
1112	22	-1.7278162355759292
1112	23	-0.6057877766395023
1112	24	-0.38607745405940364
1112	25	-1.4503521285019403
1113	1	-0.11193665716918844
1113	2	-0.1267023019560085
1113	3	-0.18435033309831894
1113	4	-0.3655711381376392
1113	5	-0.19877465253011195
1113	6	-0.06417944793108887
1113	7	-0.10827950631594518
1113	8	0.06919653143216209
1113	9	-0.1320492790869281
1113	10	-3.26877781240853E-4
1113	11	-0.06834400130322393
1113	12	-0.10116844223924915
1113	13	-0.14824201561170014
1113	14	-0.4917020294555172
1113	15	-0.4296604765842531
1113	16	-0.39377178181658934
1113	17	-0.4871953442997423
1113	18	-0.89927508857361
1113	19	-1.070098977510085
1113	20	-1.1100181078766624
1113	21	-0.9666063346336813
1113	22	-0.46324236949060804
1113	23	-1.9253997537437613
1113	24	-1.0625560703109755
1113	25	-0.4980791598134999
1114	1	-0.39215003317265484
1114	2	-0.4304887912437465
1114	3	-0.32765787803124624
1114	4	-0.5780421704463805
1114	5	-0.6738964746800491
1114	6	-0.5077015546362986
1114	7	-0.47698839780559865
1114	8	-0.2379017521589475
1114	9	-1.2003091112024364
1114	10	-0.2855119782183664
1114	11	-0.3715101429394352
1114	12	-0.39556401291266496
1114	13	-0.4354762103380878
1114	14	-0.7664088178991264
1114	15	-1.3259028297948632
1114	16	-1.2606483729393574
1114	17	-1.5582944908264338
1114	18	-1.7459272058130537
1114	19	-1.594839546718596
1114	20	-0.8582358176628873
1114	21	-0.7373393963611363
1114	22	-0.5885960440470726
1114	23	-0.42462974725175684
1114	24	-1.0259636979333635
1114	25	-0.6370014070808026
1115	1	-0.6028998778602563
1115	2	-0.3635048661071245
1115	3	-0.4108240171466129
1115	4	-0.35125995431379664
1115	5	-0.2276828054642408
1115	6	-0.2279385514352228
1115	7	-0.20585140289249892
1115	8	0.02212557619545663
1115	9	-0.3300122724120982
1115	10	-0.10304699495663527
1115	11	-0.3342063690468038
1115	12	-0.33025776376756255
1115	13	-0.29802039134825975
1115	14	-0.3113426954345542
1115	15	-1.3843053339340443
1115	16	-0.7189352814822456
1115	17	-0.5189341464312776
1115	18	-2.1395704372211384
1115	19	-1.0054127916251758
1115	20	-0.7040714841135056
1115	21	-0.7214566481681715
1115	22	-0.5113983645158626
1115	23	-0.3075341906457112
1115	24	-0.23401557297999176
1115	25	-0.35229189474052447
1116	1	-1.271644191844132
1116	2	-0.6599106785229658
1116	3	-1.443020477390018
1116	4	-0.7548360955723368
1116	5	-0.7821754328734585
1116	6	-0.3041940839018409
1116	7	-0.940664760424518
1116	8	-2.7749045086548847
1116	9	-1.3626954432254834
1116	10	-0.5101981386279206
1116	11	-0.43607627109973635
1116	12	-0.5555176164992304
1116	13	-1.5761585819140507
1116	14	-1.4697014414952534
1116	15	-1.3464137686980635
1116	16	-0.8747495588999215
1116	17	-1.654752311498605
1116	18	-2.156509819331106
1116	19	-1.2282570502290255
1116	20	-0.8148302722433627
1116	21	-0.5702221368967884
1116	22	-0.39383528912801324
1116	23	-0.322863274007716
1116	24	-0.3883083718877103
1116	25	-0.2703573179271075
1201	1	0.26608063782989433
1201	2	0.23136830758945592
1201	3	0.3334650432175579
1201	4	0.3320892376096225
1201	5	0.1913668777607711
1201	6	0.11645570378709635
1201	7	0.09417913755019214
1201	8	0.1762489053992624
1201	9	0.16501234466247183
1201	10	0.37697268666198624
1201	11	0.24932144068076667
1201	12	0.33565546251549705
1201	13	0.25158817924135235
1201	14	0.6184431301329951
1201	15	0.6209903952477589
1201	16	0.4185978826745469
1201	17	0.47411642509369756
1201	18	0.4407171026887369
1201	19	0.4768556353313045
1201	20	0.4701745487076181
1201	21	0.5770068610655201
1201	22	0.6103525606597842
1201	23	0.5707027336799442
1201	24	0.520222009224014
1201	25	0.6597444604273051
1202	1	0.2778342827594855
1202	2	0.3048491356553953
1202	3	0.2774178129492597
1202	4	0.2939573710815253
1202	5	0.14159996429659572
1202	6	-0.006436858259853295
1202	7	-0.01863151967779686
1202	8	0.14948416495143135
1202	9	0.08431854640042502
1202	10	0.3076828843227517
1202	11	0.09687769983581518
1202	12	0.18283349853579978
1202	13	0.17409130357644642
1202	14	0.5076180675502755
1202	15	0.2604982750244176
1202	16	0.1970273349192695
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2210	19	0.4134432790811573
2210	20	0.40971578000807796
2210	21	0.414341904261164
2210	22	0.5210199891133556
2210	23	0.6455404797221433
2210	24	0.6203141555004095
2210	25	0.7342932097994179
2211	1	0.1697937604710802
2211	2	-0.0014344991600054868
2211	3	0.1618480112055174
2211	4	0.20953124388275057
2211	5	0.12897431457066233
2211	6	0.1277025067597819
2211	7	0.19685599864372705
2211	8	0.22196535716061305
2211	9	0.18885219192355152
2211	10	0.4067286878187417
2211	11	0.21022052005240965
2211	12	0.21586201615210143
2211	13	0.1802548563317643
2211	14	0.16751550800010762
2211	15	0.1117753078002437
2211	16	0.11409585124074795
2211	17	0.05147764730930504
2211	18	0.2404157154708173
2211	19	0.15871570551131242
2211	20	0.23190209077484525
2211	21	0.2301975073562943
2211	22	0.2133824347028579
2211	23	0.365197782610295
2211	24	0.3677763933755216
2211	25	0.3481925092352469
2212	1	0.14396367018381184
2212	2	0.19132946682563556
2212	3	0.19261251765529863
2212	4	0.18541716828031696
2212	5	0.19043552686391507
2212	6	0.23300719882796272
2212	7	0.18113061214855009
2212	8	0.3072135848838613
2212	9	0.18096577468399744
2212	10	0.3480258529995055
2212	11	0.2774958396041285
2212	12	0.3032241548156094
2212	13	0.18041369635768945
2212	14	0.2618092323187611
2212	15	0.2747223088002855
2212	16	0.1133457401659399
2212	17	0.19242816602872637
2212	18	0.24058276621236274
2212	19	0.2621635566811946
2212	20	0.29387179517490125
2212	21	0.28528571146652126
2212	22	-0.25090049202712805
2212	23	0.25762111088557305
2212	24	0.3566464200113302
2212	25	0.3495138373080451
2213	1	0.02462126680379484
2213	2	-0.5895132793431372
2213	3	-0.03589224616800735
2213	4	0.06548143679157192
2213	5	0.12260654637923807
2213	6	-0.003067879596535761
2213	7	0.02642300734411407
2213	8	0.20455795353363015
2213	9	-0.0863581081632745
2213	10	-1.389856205214727
2213	11	-0.24575540678178953
2213	12	-0.06210334377901461
2213	13	-0.0716515638703541
2213	14	0.16549099163064085
2213	15	0.09078844975979905
2213	16	0.1662178036226507
2213	17	-0.0061543254215763454
2213	18	0.2024738960465271
2213	19	0.15129737129894494
2213	20	0.054572993580400464
2213	21	0.1410349693058066
2213	22	0.22797328726839794
2213	23	-0.7028304277521542
2213	24	-1.2183613157085844
2213	25	-1.1913169264504688
2214	1	-0.059799045857090505
2214	2	-0.15626773414823347
2214	3	-0.06601114938611019
2214	4	-0.04020220709764999
2214	5	0.002213321372586563
2214	6	-0.08820806303502593
2214	7	-0.02147724576380483
2214	8	0.005651670783535678
2214	9	-0.3288363597655035
2214	10	0.018622525670572543
2214	11	-0.11929774415084893
2214	12	-0.1430380323418845
2214	13	-0.061662596937310354
2214	14	-0.11110849143339863
2214	15	-0.13582515845900645
2214	16	-0.1804936585779302
2214	17	-0.16854043034729216
2214	18	-0.03627904261368542
2214	19	-0.6888316126647069
2214	20	-0.3301212853451716
2214	21	-0.18737796577362076
2214	22	-0.601473824866595
2214	23	-1.7459873112559734
2214	24	-1.1533310570322115
2214	25	-1.4257033082259056
2215	1	-0.4117438806806355
2215	2	-0.23849230914851915
2215	3	-1.153280962846754
2215	4	-0.8686897746028919
2215	5	-0.24344058637471022
2215	6	-0.23836518332804246
2215	7	-0.1336711482873696
2215	8	-0.03156278937176893
2215	9	-0.2380222075253542
2215	10	-1.2317210896296729
2215	11	-0.45899002038660086
2215	12	-0.36480475393004497
2215	13	-0.4494413153594934
2215	14	-0.696190314659006
2215	15	-0.9917398627850602
2215	16	-0.5276970153947005
2215	17	-0.42250865430562357
2215	18	-0.36539035305737855
2215	19	-0.9109186668535258
2215	20	-1.190654320286491
2215	21	-0.5845339052428002
2215	22	-0.30463803126965416
2215	23	-0.54930589537841
2215	24	-1.5746161360078332
2215	25	-0.609192127978595
2216	1	-0.3860979353464131
2216	2	-0.3626552225524975
2216	3	-1.2934663106906257
2216	4	-0.6923613895544776
2216	5	-0.44496244874054014
2216	6	-0.47064365776951433
2216	7	-0.2538669201305268
2216	8	-0.39544259359993106
2216	9	-0.2389429249124575
2216	10	-0.26463725534219407
2216	11	-0.3518807282841294
2216	12	-0.31378218451804685
2216	13	-0.9468351078886883
2216	14	-0.5393923928140865
2216	15	-0.9727619546034632
2216	16	-0.7092886894848291
2216	17	-1.5492435130313567
2216	18	-0.6901627174156104
2216	19	-0.4122077542552276
2216	20	-0.47366320145082597
2216	21	-0.32974602947527387
2216	22	-0.279298784081746
2216	23	-0.1758148315698307
2216	24	-0.2598670076161369
2216	25	-0.25790792033751586
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5281.0
3	520.0
4	668.0
5	573.0
6	399.0
7	355.0
8	635.0
9	1399.0
10	2318.0
11	3788.0
12	6014.0
13	8409.0
14	11141.0
15	14589.0
16	17030.0
17	18016.0
18	18342.0
19	18855.0
20	19399.0
21	20839.0
22	21954.0
23	23087.0
24	24384.0
25	26100.0
26	27414.0
27	31363.0
28	35422.0
29	41827.0
30	51712.0
31	65803.0
32	89455.0
33	128817.0
34	191243.0
35	312946.0
36	587962.0
37	2061717.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.518106326601455	22.791146791617216	13.051977962404452	21.638768919376876
2	13.440418746326413	23.016289215613046	41.692273376113924	21.85101866194662
3	17.403743899624086	30.163190208072283	30.164169153531624	22.26889673877201
4	11.25390628220879	17.810791645709482	38.859468663165096	32.07583340891663
5	13.522105947927605	37.9976473656836	34.011159033234314	14.469087653154483
6	30.647249574432127	38.94056784973848	17.525230095170578	12.886952480658817
7	23.078985817853596	33.89985327392851	26.410976222957682	16.61018468526021
8	22.684401002387446	38.82514749555156	22.830286199858083	15.660165302202914
9	23.236926127544848	16.67123572855238	23.6164502717733	36.47538787212948
10	15.662326792858508	30.35729102407313	32.519909770204684	21.460472412863673
11	31.43329238987515	28.4442997226012	21.61015988557815	18.5122480019455
12	19.398383463750825	29.74997610465414	33.58819984701826	17.263440584576774
13	24.715036106506076	26.317734161500926	27.51154465142908	21.455685080563914
14	19.092742730196385	23.442554826084034	29.255769868409153	28.20893257531043
15	24.572059493313496	29.264000032973986	23.599919419822722	22.564021053889796
16	21.684307987657988	32.83230727972093	26.242799226421614	19.240585506199473
17	19.511777906529506	31.50147215781058	30.740314144488124	18.246435791171795
18	19.540982626733953	31.540608553801064	30.53702652208009	18.381382297384892
19	20.05933051579936	28.3492694463301	31.014196300795156	20.57720373707539
20	22.58870184926044	28.24256885007038	28.34625871510095	20.822470585568226
21	22.711539908106097	30.935647476936364	27.323829661389826	19.028982953567716
22	19.563168798595694	30.073208795772246	30.288419676158373	20.07520272947369
23	18.75469520457953	31.559065544798305	29.968131862469793	19.71810738815237
24	19.647094897357075	28.73406631915546	29.63596354482044	21.98287523866702
25	21.8585731165558	27.804006684506444	27.058982060767583	23.27843813817017
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8648.0
1	8648.0
2	7262.5
3	5877.0
4	5877.0
5	5877.0
6	8016.5
7	10156.0
8	10156.0
9	10156.0
10	13901.0
11	17646.0
12	17646.0
13	17646.0
14	27807.0
15	37968.0
16	37968.0
17	37968.0
18	58962.5
19	79957.0
20	79957.0
21	79957.0
22	112019.5
23	144082.0
24	144082.0
25	144082.0
26	187256.0
27	230430.0
28	230430.0
29	230430.0
30	279635.0
31	328840.0
32	328840.0
33	328840.0
34	385663.5
35	442487.0
36	442487.0
37	442487.0
38	498291.0
39	554095.0
40	554095.0
41	554095.0
42	530795.0
43	507495.0
44	507495.0
45	507495.0
46	532241.0
47	556987.0
48	556987.0
49	556987.0
50	479293.5
51	401600.0
52	401600.0
53	401600.0
54	322715.5
55	243831.0
56	243831.0
57	243831.0
58	196465.0
59	149099.0
60	149099.0
61	149099.0
62	119450.5
63	89802.0
64	89802.0
65	89802.0
66	67497.5
67	45193.0
68	45193.0
69	45193.0
70	32849.0
71	20505.0
72	20505.0
73	20505.0
74	14680.5
75	8856.0
76	8856.0
77	8856.0
78	6241.0
79	3626.0
80	3626.0
81	3626.0
82	2626.5
83	1627.0
84	1627.0
85	1627.0
86	1112.0
87	597.0
88	597.0
89	597.0
90	431.0
91	265.0
92	265.0
93	265.0
94	176.5
95	88.0
96	88.0
97	88.0
98	53.5
99	19.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3188358404185742
2	0.18844272780746243
3	0.20690137426936667
4	0.22746811127427388
5	0.2537421177980429
6	0.20319936160848334
7	0.19826334472730564
8	0.2221721764955103
9	0.22109242280275265
10	0.23183854288781666
11	0.20566737004907223
12	0.21253151852446
13	0.2251543533612218
14	0.2127371858945091
15	0.20381636371863057
16	0.2075955016432823
17	0.2071841669031841
18	0.21029488587517636
19	0.20903517323362578
20	0.2058730374191213
21	0.20330219529350793
22	0.2057187868915845
23	0.20967788376502913
24	0.2274166944317616
25	0.22011550279501957
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3889776.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.48687615792975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.99949982793532	48.994067149465764
2	12.677762893260766	15.336765481685221
3	3.3772061075951703	6.128299427697391
4	1.2071070099286887	2.920565288757019
5	0.5245860995795142	1.5865287219718738
6	0.29284100401928115	1.0627822526446842
7	0.1831600944434259	0.7755147364771954
8	0.1273677969508113	0.6163264128537652
9	0.08387650683691873	0.4566085093444006
>10	0.4437276010433616	4.980117346735756
>50	0.039964070626595356	1.680620479717761
>100	0.034871440977704504	4.252129131335999
>500	0.004809140348140091	2.0597600452709064
>1k	0.002576325163382598	3.594256061653008
>5k	3.435100217843463E-4	1.6937322639866002
>10k+	3.0057126906130304E-4	3.8619266904025813
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	38318	0.9850952856925437	No Hit
CCCATGTACTCTGCGTTGATACCAC	36041	0.9265572104923266	No Hit
ATTCCATTCCATTCCATTCCATTCC	18441	0.47408899638436763	No Hit
GAGTACATGGGAAGCAGTGGTATCA	17422	0.44789211512436705	No Hit
GAATGGAATGGAATGGAATGGAATG	13649	0.35089424172497335	No Hit
CATGTACTCTGCGTTGATACCACTG	13121	0.3373201953017346	No Hit
GCGTTGATACCACTGCTTCCCATGT	11702	0.3008399455392804	No Hit
GTATCAACGCAGAGTACATGGGAAG	9944	0.25564454097099676	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	9478	0.24366441666563832	No Hit
GGTATCAACGCAGAGTACATGGGAA	8940	0.2298332860298382	No Hit
GCTTCCCATGTACTCTGCGTTGATA	8823	0.22682540074287055	No Hit
TATCAACGCAGAGTACATGGGAAGC	8682	0.22320051334575564	No Hit
ACTCTGCGTTGATACCACTGCTTCC	7986	0.20530745215148635	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5712	0.14684650221503756	No Hit
GTGGTATCAACGCAGAGTACATGGG	5648	0.145201163254645	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	4787	0.1230662125531136	No Hit
CAGTGGTATCAACGCAGAGTACATG	4451	0.11442818301105256	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4410	0.11337413773955106	No Hit
ACATGGGAAGCAGTGGTATCAACGC	4044	0.10396485555980602	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.570842125613403E-5	0.0
10	0.0	0.0	0.0	2.570842125613403E-5	0.0
11	0.0	0.0	0.0	2.570842125613403E-5	0.0
12	0.0	0.0	0.0	5.141684251226806E-5	0.0
13	0.0	0.0	0.0	5.141684251226806E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAC	90	1.3278623E-10	15.85311	1
TCGTACC	90	1.3460522E-10	15.840028	2
CGTACCC	95	4.8803486E-9	14.005919	3
TACGACC	160	0.0	13.069709	4
GTACGAC	150	1.8189894E-12	12.672022	3
CCGTTCG	55	0.0030720069	12.089786	9
AGCGTAA	55	0.0030723035	12.089629	10
ATAGCCG	55	0.0030764574	12.087448	16
TCGGAAC	210	0.0	10.854035	13
GTGCTAG	360	0.0	10.832959	1
TATTCCG	540	0.0	10.736989	5
GGTACGA	160	5.4205884E-10	10.692019	2
GGGTACG	180	3.45608E-11	10.568741	1
ACGACCA	180	3.45608E-11	10.560972	5
GTCCTAC	155	3.594323E-9	10.43237	1
ACTCGGA	250	0.0	10.256799	11
GTCTAGC	295	0.0	9.995521	1
CTCGGAA	250	0.0	9.877299	12
CGACCAT	175	2.582965E-9	9.770143	6
GGAACGA	500	0.0	9.687102	15
>>END_MODULE