FastQCFastQC Report
Wed 25 May 2016
SRR1295053_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295053_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3889776
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC398411.0242492112656358No Hit
GTACATGGGAAGCAGTGGTATCAAC367700.9452986495880482No Hit
GAGTACATGGGAAGCAGTGGTATCA188180.4837810711979302No Hit
ATTCCATTCCATTCCATTCCATTCC179920.4625459152403634No Hit
GAATGGAATGGAATGGAATGGAATG151420.38927691466038145No Hit
GTATCAACGCAGAGTACTTTTTTTT149020.3831068935589093No Hit
CATGTACTCTGCGTTGATACCACTG147540.3793020472130015No Hit
GCGTTGATACCACTGCTTCCCATGT126850.32611132363406015No Hit
GGTATCAACGCAGAGTACTTTTTTT122830.3157765382890943No Hit
ACGCAGAGTACATGGGAAGCAGTGG113620.29209908231219484No Hit
TATCAACGCAGAGTACTTTTTTTTT105600.27148092846477534No Hit
GTATCAACGCAGAGTACATGGGAAG103920.26716191369374487No Hit
GCTTCCCATGTACTCTGCGTTGATA96540.24818909880671794No Hit
GGTATCAACGCAGAGTACATGGGAA96510.2481119735429495No Hit
TATCAACGCAGAGTACATGGGAAGC93600.2406308229574145No Hit
ACTCTGCGTTGATACCACTGCTTCC88700.22803369654190883No Hit
GTGGTATCAACGCAGAGTACATGGG61190.15730982966628412No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56820.14607524957735354No Hit
CAGTGGTATCAACGCAGAGTACATG51460.13229553578406572No Hit
ACATGGGAAGCAGTGGTATCAACGC48020.12345183887195561No Hit
CATGGGAAGCAGTGGTATCAACGCA43530.11190875772795143No Hit
GCAGTGGTATCAACGCAGAGTACAT42390.10897799770475215No Hit
ATACCACTGCTTCCCATGTACTCTG42050.10810391138204359No Hit
GGGAAGCAGTGGTATCAACGCAGAG41030.10548165241391792No Hit
GGAATGGAATGGAATGGAATGGAAT39520.10159968080424167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG957.40547E-812.9989889
GTCGTAC1209.824362E-911.8916551
GTTCTAG3100.011.3546111
TCGTACC1201.2785313E-711.0824712
CTTACAC7200.011.0824713
CGTACCC1303.2750904E-810.9606863
ACGCCGA700.001493244110.8562987
AACGCCG700.001493244110.8562986
GCCCTAG1701.364242E-1010.6325391
CGCAAAA1409.7134034E-810.1777792
GCAGGAC2400.09.8950633
TTACACT10000.09.8792314
GCTCTAG2251.8189894E-129.7247311
TAGACAG5450.09.58641055
CGAATTG1606.652408E-89.49926119
CGCCGAG800.00452092489.4992618
ACGCAAA1951.6425474E-99.2693931
CCTTACA7900.09.2587732
ACGAATT1752.6768248E-89.22785418
CTGTCGC1651.0683107E-79.2114059
Produced by FastQC (version 0.11.5)