FastQCFastQC Report
Wed 25 May 2016
SRR1295052_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295052_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5057481
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC338350.6690089394305189No Hit
CCCATGTACTCTGCGTTGATACCAC313180.6192410806881924No Hit
GAGTACATGGGAAGCAGTGGTATCA159560.31549302903955545No Hit
CATGTACTCTGCGTTGATACCACTG125640.24842406723821603No Hit
GCGTTGATACCACTGCTTCCCATGT102740.20314460894662778No Hit
GTATCAACGCAGAGTACATGGGAAG91130.18018851677346884No Hit
ACGCAGAGTACATGGGAAGCAGTGG86150.17034171754673919No Hit
GCTTCCCATGTACTCTGCGTTGATA85080.16822603980123702No Hit
TATCAACGCAGAGTACATGGGAAGC78180.15458288424612965No Hit
GGTATCAACGCAGAGTACATGGGAA75140.14857198672619829No Hit
ACTCTGCGTTGATACCACTGCTTCC73700.145724719479915No Hit
GTATCAACGCAGAGTACTTTTTTTT67450.13336678872347715No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN61160.12092976721019813No Hit
GGTATCAACGCAGAGTACTTTTTTT53140.10507207046353709No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCGT701.09265166E-412.2133118
GTACGGA802.8320273E-511.8902031
GACGGCG802.871987E-511.8739357
AGACCGT1001.934768E-611.398756
CTTAACG600.005857556611.0887152
TAGGACG1553.6288839E-910.4263794
ACACCGT1856.002665E-1110.2691456
GCGGTTG1151.0310061E-59.91028718
CCGTGCA1356.459177E-79.8510669
CTAGGAC5500.09.8503333
GTCTACG900.00110146199.5121621
TACACCG1002.741177E-49.50565055
AGGACCG1304.2117445E-69.505655
CGGCGTG900.0011152419.4992429
CACTCGT800.00452324149.49877110
CCTACAC5450.09.4175083
TCTGTCG2800.09.1599838
GTGTTAG7650.09.0769651
CTAGGCG950.00181377929.0060684
ACTCCGC950.00182626258.9992818
Produced by FastQC (version 0.11.5)