##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295052_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5057481
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.371847763738508	33.0	33.0	33.0	27.0	33.0
2	31.34127028850924	33.0	33.0	33.0	27.0	33.0
3	31.419464353894757	33.0	33.0	33.0	27.0	33.0
4	34.97556669021594	37.0	37.0	37.0	33.0	37.0
5	35.329993132945035	37.0	37.0	37.0	33.0	37.0
6	35.375937349047874	37.0	37.0	37.0	33.0	37.0
7	35.21670728965665	37.0	37.0	37.0	33.0	37.0
8	35.130137710848544	37.0	37.0	37.0	33.0	37.0
9	35.097687366497276	37.0	37.0	37.0	33.0	37.0
10	34.99309893601182	37.0	37.0	37.0	33.0	37.0
11	35.185722497029644	37.0	37.0	37.0	33.0	37.0
12	35.09635567587896	37.0	37.0	37.0	33.0	37.0
13	35.03863583471693	37.0	37.0	37.0	33.0	37.0
14	36.528437378212594	40.0	37.0	40.0	33.0	40.0
15	36.69017283505366	40.0	37.0	40.0	33.0	40.0
16	36.76840268900664	40.0	37.0	40.0	33.0	40.0
17	36.693381547058706	40.0	37.0	40.0	33.0	40.0
18	36.622934223578895	40.0	37.0	40.0	33.0	40.0
19	36.71617807362993	40.0	37.0	40.0	33.0	40.0
20	36.71812291534066	40.0	37.0	40.0	33.0	40.0
21	36.72960906822981	40.0	37.0	40.0	33.0	40.0
22	36.640513330648204	40.0	37.0	40.0	33.0	40.0
23	36.533723804399855	40.0	37.0	40.0	33.0	40.0
24	36.58698292687605	40.0	37.0	40.0	33.0	40.0
25	35.590275672810236	37.0	37.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.39903082568485715
1101	2	0.34109420142270963
1101	3	0.34308547193544214
1101	4	0.4436076743537001
1101	5	0.39960209468940633
1101	6	0.3283108752943633
1101	7	0.2796304409719639
1101	8	0.42507544567040867
1101	9	0.4328300483054761
1101	10	0.6094253039737438
1101	11	0.41954328737512725
1101	12	0.49826440098939884
1101	13	0.4743865325018959
1101	14	0.9434895072718774
1101	15	0.7628756187580663
1101	16	0.7635517909296681
1101	17	0.7557120421273424
1101	18	0.745136103079119
1101	19	0.6890393163546449
1101	20	0.7419038998876886
1101	21	0.6807227221682197
1101	22	0.7807170718877572
1101	23	0.8188124667318846
1101	24	0.8114132514107837
1101	25	0.9013256611674407
1102	1	0.28276763520919346
1102	2	0.28706225360275184
1102	3	0.3279296232599691
1102	4	0.3402718792903272
1102	5	0.3373439397895126
1102	6	0.2582645371503034
1102	7	0.28615414066499056
1102	8	0.4186352821805741
1102	9	0.4469293207809173
1102	10	0.5511251152838241
1102	11	0.3854387365911549
1102	12	0.43307829843497103
1102	13	0.4317496991839249
1102	14	0.8463313040176601
1102	15	0.7521430829615667
1102	16	0.6716764873387433
1102	17	0.6670968900374277
1102	18	0.6789200752849283
1102	19	0.5699638896766004
1102	20	0.6915786004689082
1102	21	0.6917623039019247
1102	22	0.7196446234358547
1102	23	0.77542638309815
1102	24	0.7951491669708375
1102	25	0.8087098229459357
1103	1	0.2648342657028735
1103	2	0.22242818365423034
1103	3	0.32533605305384583
1103	4	0.34533830103751484
1103	5	0.2756909816617181
1103	6	0.19001243201584828
1103	7	0.19307033396898987
1103	8	0.36559106244046546
1103	9	0.3686134977416984
1103	10	0.4536668549950278
1103	11	0.346456049766239
1103	12	0.4230078962591861
1103	13	0.36008213245398935
1103	14	0.7308104449829074
1103	15	0.7034844572659509
1103	16	0.5818995502287834
1103	17	0.5364755870465459
1103	18	0.6517336292332985
1103	19	0.567614569308077
1103	20	0.6320283386774292
1103	21	0.588581613628584
1103	22	0.6266029130368338
1103	23	0.748884549149686
1103	24	0.6965287566771323
1103	25	0.7868718164366229
1104	1	0.26058333152204227
1104	2	0.26228090285205496
1104	3	0.21607323091600605
1104	4	0.23599542531714235
1104	5	0.2936561619112581
1104	6	0.1799043329949015
1104	7	0.16575960954633473
1104	8	0.3461864298285917
1104	9	0.2992882515991724
1104	10	0.5038464863393841
1104	11	0.3297343684986842
1104	12	0.3157959579573699
1104	13	0.31139309881636734
1104	14	0.673021542722104
1104	15	0.5973356305401509
1104	16	0.4256284638927781
1104	17	0.5782321583338117
1104	18	0.5492183456701625
1104	19	0.46523155205296973
1104	20	0.5425059294559631
1104	21	0.45381238586991657
1104	22	0.49570561021992177
1104	23	0.5250493065384063
1104	24	0.6147188874935878
1104	25	0.7196649659847196
1105	1	0.16977914931797145
1105	2	0.05189536384154536
1105	3	0.12468512770536577
1105	4	0.20156681070395166
1105	5	0.1552018799295709
1105	6	0.09119267973056822
1105	7	0.11707949615499302
1105	8	0.26923574576003517
1105	9	0.26130632783603147
1105	10	0.4418894499036412
1105	11	0.25323860270549403
1105	12	0.349957371501759
1105	13	0.3405323900570707
1105	14	0.5844428029641051
1105	15	0.48552440259903307
1105	16	0.4428488751794859
1105	17	0.3903281858318479
1105	18	0.3976840006087343
1105	19	0.42393276249072187
1105	20	0.44460517013141043
1105	21	0.4681403033081679
1105	22	0.4291593848578046
1105	23	0.48036188316846307
1105	24	0.49707553116066805
1105	25	0.4570363860400235
1106	1	-0.05691520722211507
1106	2	-0.15083589787903406
1106	3	-0.18052430443247047
1106	4	-0.11517091198709295
1106	5	-0.3222506517159971
1106	6	-0.23041091555775495
1106	7	-0.4517422924057186
1106	8	-0.22032744282412153
1106	9	0.05719057244456138
1106	10	-0.34142263987601496
1106	11	-0.34017443058095864
1106	12	-0.6077551372886774
1106	13	-0.1906040668111899
1106	14	-0.36131837554034263
1106	15	-0.1863322839926198
1106	16	-0.10462863595506633
1106	17	-0.2109742637696499
1106	18	-0.2946580645577015
1106	19	-0.4337885367357188
1106	20	-0.11790215523193126
1106	21	0.021653913833247884
1106	22	0.14367898753010167
1106	23	-0.4853993685896256
1106	24	0.0631466457879526
1106	25	0.17535052692140596
1107	1	-1.2324488380983176
1107	2	-1.2929108046821156
1107	3	-0.8704766113673159
1107	4	-0.9889583299880229
1107	5	-1.075103437314496
1107	6	-1.0229879629868748
1107	7	-1.1094179446064203
1107	8	-0.9278715573171041
1107	9	-0.9967745847637701
1107	10	-0.9122135665366145
1107	11	-0.9201516675440544
1107	12	-0.9882266423152615
1107	13	-0.9176809865611162
1107	14	-1.2006705902854833
1107	15	-1.0787510877304527
1107	16	-1.076562283953912
1107	17	-1.2059895911971594
1107	18	-1.298650927557631
1107	19	-1.2564426982478238
1107	20	-1.3040511553646468
1107	21	-1.2779845926105509
1107	22	-1.2255805590813935
1107	23	-1.0595789038966217
1107	24	-0.9262076204151626
1107	25	-0.9588504472322938
1108	1	0.06293479070388486
1108	2	-0.18222855741575117
1108	3	-0.01977221142046659
1108	4	-0.16950525581948028
1108	5	-0.4176235569756628
1108	6	-0.04439922683755526
1108	7	-0.008719978466594114
1108	8	0.16469595593240172
1108	9	-0.2346213462592317
1108	10	-0.488462206041234
1108	11	-0.09532544084956385
1108	12	0.07925077534060421
1108	13	0.04484610072195494
1108	14	-0.2685361703479714
1108	15	-0.02118150992960466
1108	16	-0.7330459115712742
1108	17	-0.7347748578555269
1108	18	-0.6431197900338006
1108	19	-0.04814351399085126
1108	20	-1.055475033299146
1108	21	-0.19210890752157894
1108	22	-0.2871846434914147
1108	23	-0.08334057759355318
1108	24	-0.4439667432668557
1108	25	-1.086260274241873
1109	1	0.12784433327632883
1109	2	0.05650536423588193
1109	3	0.0642947830545495
1109	4	0.068265073648071
1109	5	0.04894093706904812
1109	6	0.0988694907246952
1109	7	0.07631867159566497
1109	8	0.13359351814853682
1109	9	0.163595308079735
1109	10	0.2082029875037037
1109	11	0.0536127795263468
1109	12	0.20849275341745255
1109	13	0.15078752630061842
1109	14	0.3662657257922959
1109	15	0.24195096713233255
1109	16	0.1943849176101793
1109	17	0.19842107198666525
1109	18	0.22269114797258993
1109	19	0.18133915667030465
1109	20	0.10718719229316775
1109	21	0.16453201828115027
1109	22	0.24130522065574667
1109	23	0.26297550160767713
1109	24	0.32261542823862754
1109	25	0.33277729656488475
1110	1	0.016153453607977042
1110	2	-0.06895551615976814
1110	3	0.10653356143437165
1110	4	-1.046650817232809
1110	5	-0.28617737026333856
1110	6	-0.023092565471287685
1110	7	0.0011755451789454696
1110	8	0.13123787188261105
1110	9	0.10042390487718933
1110	10	0.018338537887352402
1110	11	0.05447897769113297
1110	12	0.09104140670181238
1110	13	0.1699474806019836
1110	14	0.2314459141627836
1110	15	0.1426630233283177
1110	16	0.10501241012505602
1110	17	0.19941222924087043
1110	18	0.27039318562244574
1110	19	0.13317164754875677
1110	20	0.14383629771518258
1110	21	0.24912556410706088
1110	22	-0.3402738064603881
1110	23	0.10395968891145912
1110	24	0.20399794820905015
1110	25	0.19319914523816806
1111	1	0.007404840717502026
1111	2	-0.17089577281670287
1111	3	-0.023137544776545838
1111	4	-0.040611562628555475
1111	5	-0.11480981124272205
1111	6	-0.06465087288809457
1111	7	-0.04221627694717256
1111	8	0.08520342751224774
1111	9	0.013845558788005974
1111	10	0.0693221949986409
1111	11	-0.08210317848430293
1111	12	0.0326906203952646
1111	13	-0.08286183291572513
1111	14	-0.15825708961396856
1111	15	-0.08128509520346228
1111	16	-0.07964368791798648
1111	17	-0.05383842918966053
1111	18	-0.05947443769338179
1111	19	0.002828001581974604
1111	20	-0.061437248617849605
1111	21	-0.1289044962828072
1111	22	-0.08391965418076808
1111	23	0.08530306579090308
1111	24	0.08012207248453507
1111	25	-0.06268234798226047
1112	1	-0.046900872359728396
1112	2	-0.447824568028544
1112	3	-0.0986227052989328
1112	4	-0.20914782754944383
1112	5	-0.09782404621141438
1112	6	-0.08686431132738193
1112	7	-0.06838623566090263
1112	8	-0.036405052830915
1112	9	-0.1311876236988283
1112	10	0.14107789104481583
1112	11	-0.09465667658842847
1112	12	0.030274456238338132
1112	13	-0.047253000722164984
1112	14	-0.18554084198614618
1112	15	-0.1614283806626844
1112	16	-0.17561365336926826
1112	17	-0.25730729978732114
1112	18	-0.20051157288254018
1112	19	-0.20569905533918842
1112	20	-1.008509741028739
1112	21	-1.287811289615611
1112	22	-1.6262920241926366
1112	23	-0.5680548222083814
1112	24	-0.3322875412660764
1112	25	-1.3687060267653095
1113	1	-0.18777318613562244
1113	2	-0.16255671615531853
1113	3	-0.16796542060671982
1113	4	-0.44462891605084565
1113	5	-0.22728698719372176
1113	6	-0.1753787373153628
1113	7	-0.16326532142115013
1113	8	-0.08116419708642297
1113	9	-0.19284407118315983
1113	10	-0.05657652790449674
1113	11	-0.17252409171457117
1113	12	-0.16455693271795013
1113	13	-0.1765503363026255
1113	14	-0.519419999881805
1113	15	-0.4959952294166001
1113	16	-0.5252286470410326
1113	17	-0.4523896762335795
1113	18	-0.8615283323415639
1113	19	-1.080701820529697
1113	20	-1.1692082303384836
1113	21	-1.0094410688594166
1113	22	-0.4655799821704818
1113	23	-1.8928233222072848
1113	24	-1.0824341510113484
1113	25	-0.5873323500674488
1114	1	-0.13724373286314062
1114	2	-0.31476675092955375
1114	3	-0.21259920150355427
1114	4	-0.4092245957130345
1114	5	-0.44674252163469674
1114	6	-0.30716868734220526
1114	7	-0.2604693179772539
1114	8	-0.031036230000133003
1114	9	-1.0452858559655525
1114	10	-0.2735997601247746
1114	11	-0.2959562560274165
1114	12	-0.28635878931077485
1114	13	-0.3146491514126737
1114	14	-0.6198423119415679
1114	15	-1.0812932187782494
1114	16	-1.064810183884326
1114	17	-1.439794576812524
1114	18	-1.5860066070495336
1114	19	-1.4090554678906102
1114	20	-0.7521668432313717
1114	21	-0.5792226648444014
1114	22	-0.3538797080087832
1114	23	-0.26849296875639794
1114	24	-0.7918196743079449
1114	25	-0.43644406257897117
1115	1	-0.6608411217128207
1115	2	-0.4775634529967867
1115	3	-0.49889087749371086
1115	4	-0.6612485097418741
1115	5	-0.4609386074911086
1115	6	-0.5188850123389059
1115	7	-0.2969065838142768
1115	8	-0.29933142309673855
1115	9	-0.6174073367075295
1115	10	-0.327804724810278
1115	11	-0.5585192971483011
1115	12	-0.598565816638633
1115	13	-0.4566813705175008
1115	14	-0.5480202585940077
1115	15	-1.5515954001875016
1115	16	-0.8900431993584519
1115	17	-0.7567286124196428
1115	18	-2.267987460908884
1115	19	-1.2465553244523377
1115	20	-0.9927816770437872
1115	21	-0.9318197582112404
1115	22	-0.819296023251681
1115	23	-0.5189699010133708
1115	24	-0.561254843077414
1115	25	-0.65691585974578
1116	1	-1.3957364172032811
1116	2	-0.7836802756254251
1116	3	-1.6412337559390586
1116	4	-0.9545610171346155
1116	5	-0.937599050962703
1116	6	-0.48931951960672393
1116	7	-1.141468830384376
1116	8	-2.9874114627550696
1116	9	-1.3555208015353486
1116	10	-0.705971643328617
1116	11	-0.5079200363562251
1116	12	-0.6598752526370717
1116	13	-1.6016161116950514
1116	14	-1.624160203067028
1116	15	-1.465556821029658
1116	16	-0.9665023608452117
1116	17	-1.6063269787727918
1116	18	-2.2950209804396806
1116	19	-1.3131803093345908
1116	20	-0.9656638470084502
1116	21	-0.818018186763112
1116	22	-0.5435120435433447
1116	23	-0.4193682951432933
1116	24	-0.6227790839672309
1116	25	-0.45296966297187424
1201	1	0.3180457213165404
1201	2	0.244742913360799
1201	3	0.29998769898956823
1201	4	0.3154652160111695
1201	5	0.28650995851570826
1201	6	0.15223412678758308
1201	7	0.13659083382098913
1201	8	0.29250255370286027
1201	9	0.29271332151417795
1201	10	0.5715782793587749
1201	11	0.3472288665565131
1201	12	0.4160543779862351
1201	13	0.37653269206572304
1201	14	0.7553129436014032
1201	15	0.6247047068437084
1201	16	0.5248103560091977
1201	17	0.56788230055448
1201	18	0.49553217084287837
1201	19	0.49921794649820583
1201	20	0.5668477077590737
1201	21	0.5127210267350364
1201	22	0.5661610887383262
1201	23	0.6626372606066724
1201	24	0.5581943679337016
1201	25	0.6714658837183265
1202	1	0.18121532045637423
1202	2	0.25775529035862377
1202	3	0.3514487569658158
1202	4	0.33556202253871703
1202	5	0.3330226962814322
1202	6	0.07114769406403099
1202	7	0.05681351646198607
1202	8	0.2465945965423657
1202	9	0.2324063253417421
1202	10	0.39461440288918936
1202	11	0.19353231541442284
1202	12	0.3072003596486681
1202	13	0.1921842476064768
1202	14	0.6192251588260973
1202	15	0.40528453173360646
1202	16	0.37333412174867675
1202	17	0.38618860830644053
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2210	22	0.474129613293222
2210	23	0.5253759671857097
2210	24	0.5038346036634067
2210	25	0.6445246976580776
2211	1	0.1696665614916597
2211	2	0.054396296544855005
2211	3	0.16992109188643667
2211	4	0.19596068818966472
2211	5	0.19890639600875204
2211	6	0.11791877598867018
2211	7	0.1629213272350114
2211	8	0.25908698738716396
2211	9	0.19622124695231236
2211	10	0.3504722325907963
2211	11	0.21431106906963748
2211	12	0.22373466185425173
2211	13	0.15967770601324816
2211	14	0.13215418709582138
2211	15	0.19441974821439345
2211	16	0.1262219557229045
2211	17	0.1678865441803623
2211	18	0.18844006200949082
2211	19	0.10915998111481429
2211	20	0.14653846116137714
2211	21	0.2152031659502356
2211	22	0.14524649739568218
2211	23	0.28225077322810677
2211	24	0.27349645001781653
2211	25	0.2300136198839624
2212	1	0.24528743135598674
2212	2	0.20475795053671675
2212	3	0.19353255029279737
2212	4	0.29890903568080773
2212	5	0.2967699779703352
2212	6	0.21877982275666596
2212	7	0.22603124613399928
2212	8	0.2890914523731922
2212	9	0.1929186561290308
2212	10	0.41056595125751727
2212	11	0.258479181958684
2212	12	0.25559869773599075
2212	13	0.14627035932411303
2212	14	0.20020855916066438
2212	15	0.15147886381311793
2212	16	0.14233289698067608
2212	17	0.23957993141304712
2212	18	0.32573050318504926
2212	19	0.2809933626446721
2212	20	0.20519900689180304
2212	21	0.2630815031489533
2212	22	-0.3916340358999406
2212	23	0.20384185414294365
2212	24	0.2510796001252231
2212	25	0.2127696065460185
2213	1	-0.032977471817218884
2213	2	-0.716236772800567
2213	3	-0.14700117590139428
2213	4	-0.028125047266442493
2213	5	0.006256881240688017
2213	6	-0.038166035614253246
2213	7	-0.03530122209811992
2213	8	0.09945294402459126
2213	9	-0.2279313476747049
2213	10	-1.482219289279655
2213	11	-0.47190308720367824
2213	12	-0.10926319990637978
2213	13	-0.13245415312631792
2213	14	-0.0403560599408479
2213	15	-0.09550032385451601
2213	16	-0.13293362261545383
2213	17	-0.14587500137166387
2213	18	-0.03325073561858716
2213	19	-0.02963511159448018
2213	20	-0.19847928956965433
2213	21	-0.09779690616047532
2213	22	-0.04033407744165629
2213	23	-0.9167788412826567
2213	24	-1.5026048843438744
2213	25	-1.3741090226705595
2214	1	0.005237915706977958
2214	2	-0.14353511854090328
2214	3	-0.0472690463567389
2214	4	-0.08897294591641725
2214	5	-0.07223846990490301
2214	6	-0.0583976012442875
2214	7	-0.07163777517359904
2214	8	-0.02795043783336837
2214	9	-0.38987862931113426
2214	10	-0.025668505910736883
2214	11	-0.18356655604169703
2214	12	-0.2025231813933246
2214	13	-0.07411573509126868
2214	14	-0.12420309405388252
2214	15	-0.15866289049521498
2214	16	-0.3141016961505372
2214	17	-0.22362920287142884
2214	18	-0.042630537454520834
2214	19	-0.7538790739860772
2214	20	-0.3616130245476228
2214	21	-0.3034341775801437
2214	22	-0.4972669016771434
2214	23	-1.772713068091953
2214	24	-1.2731445327941486
2214	25	-1.5871894632573458
2215	1	-0.3948128455061699
2215	2	-0.16187566913522033
2215	3	-1.1866190969745531
2215	4	-0.9139758749118556
2215	5	-0.30893317138900755
2215	6	-0.2634072730271342
2215	7	-0.11939512960574206
2215	8	-0.08793491087405414
2215	9	-0.3532029023031029
2215	10	-1.4310407378760317
2215	11	-0.534599509739877
2215	12	-0.40767589215280964
2215	13	-0.41745213845319284
2215	14	-0.7858491055956947
2215	15	-1.0985501742270003
2215	16	-0.6765101551590647
2215	17	-0.5089623697704511
2215	18	-0.43068898216451146
2215	19	-0.9812891614651349
2215	20	-1.1362619049369371
2215	21	-0.6532695601291962
2215	22	-0.33258189151592177
2215	23	-0.654545852914886
2215	24	-1.6706782342003308
2215	25	-0.7231322029577925
2216	1	-0.4358843164175177
2216	2	-0.43269433497992793
2216	3	-1.3660876425550725
2216	4	-0.673496662306377
2216	5	-0.5560724907964598
2216	6	-0.5379905372052676
2216	7	-0.38653555549639407
2216	8	-0.46960446230049513
2216	9	-0.2173618301735729
2216	10	-0.3626435051378252
2216	11	-0.38006108447623177
2216	12	-0.3702665118097457
2216	13	-1.1017315412684
2216	14	-0.7122496893973036
2216	15	-1.1170510739032196
2216	16	-0.8843303042181603
2216	17	-1.807051051764546
2216	18	-0.9176042650685545
2216	19	-0.6300594898008569
2216	20	-0.6007094668538997
2216	21	-0.4602472276099192
2216	22	-0.5891814051902102
2216	23	-0.471280594643261
2216	24	-0.6016653752848455
2216	25	-0.4396730203327408
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6729.0
3	686.0
4	855.0
5	730.0
6	477.0
7	409.0
8	563.0
9	1091.0
10	1885.0
11	3306.0
12	5758.0
13	8586.0
14	11822.0
15	15338.0
16	18362.0
17	19239.0
18	19595.0
19	20464.0
20	21350.0
21	22899.0
22	25047.0
23	27009.0
24	28954.0
25	31365.0
26	33700.0
27	38488.0
28	43434.0
29	52501.0
30	64280.0
31	82945.0
32	114179.0
33	165125.0
34	245293.0
35	404332.0
36	761894.0
37	2758791.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.72847346369795	20.674099091102573	13.079292439329315	23.518135005870157
2	15.06959378402235	20.97646822044978	39.99790418355073	23.956033811977136
3	18.48779853798018	25.968544542151946	29.79770822370298	25.745948696164895
4	12.242214183161769	17.749175368767954	37.96634890248989	32.04226154558038
5	12.632559212705466	38.83444031017156	35.42775848736754	13.105241989755434
6	31.20829529388643	38.249713911659725	17.393477661334806	13.148513133119044
7	26.25777469602041	33.00516579579505	23.037428493598476	17.699631014586068
8	24.348283580093156	37.135961144616815	21.149689729619404	17.36606554567063
9	24.282987300424125	17.031742895761703	21.842549197059522	36.84272060675465
10	14.419051529850657	30.538093467664133	34.220524105740715	20.822330896744496
11	32.0854245022554	25.672365582854866	22.913696795679677	19.328513119210054
12	20.746846611753455	27.864739215748045	31.646883129414988	19.74153104308351
13	26.803888568190544	23.542911519557283	26.40878556258978	23.244414349662396
14	20.075643867498798	23.714281128642327	28.168523187036875	28.041551816822004
15	23.55914080112883	30.205495831181217	23.90161734279334	22.333746024896612
16	21.18812814953976	31.15667287345017	26.601185695100977	21.054013281909096
17	20.536533697259614	30.36107716473529	28.48281890704822	20.619570230956878
18	20.778864388252085	29.694318731425806	29.28019062454862	20.246626255773492
19	21.057552659683022	29.34365359429209	29.482507534133934	20.116286211890955
20	22.142996824181633	28.697617274359683	28.616067156403258	20.54331874505543
21	21.773203409075375	29.122860684743344	28.376964471066106	20.72697143511517
22	21.07316100882413	28.886390685655737	28.454109946082795	21.586338359437335
23	20.637762815272325	29.30499510004585	28.68032524758341	21.37691683709842
24	21.08345572126506	29.339255779646635	28.473599382336463	21.103689116751845
25	21.57918933111395	28.40802940681073	27.505751567933896	22.507029694141426
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10409.0
1	10409.0
2	8417.5
3	6426.0
4	6426.0
5	6426.0
6	8945.0
7	11464.0
8	11464.0
9	11464.0
10	16699.5
11	21935.0
12	21935.0
13	21935.0
14	35300.0
15	48665.0
16	48665.0
17	48665.0
18	75768.0
19	102871.0
20	102871.0
21	102871.0
22	145799.0
23	188727.0
24	188727.0
25	188727.0
26	243860.5
27	298994.0
28	298994.0
29	298994.0
30	355644.5
31	412295.0
32	412295.0
33	412295.0
34	459590.5
35	506886.0
36	506886.0
37	506886.0
38	537205.0
39	567524.0
40	567524.0
41	567524.0
42	568860.0
43	570196.0
44	570196.0
45	570196.0
46	616351.5
47	662507.0
48	662507.0
49	662507.0
50	608685.0
51	554863.0
52	554863.0
53	554863.0
54	484001.5
55	413140.0
56	413140.0
57	413140.0
58	355134.5
59	297129.0
60	297129.0
61	297129.0
62	246248.0
63	195367.0
64	195367.0
65	195367.0
66	150758.0
67	106149.0
68	106149.0
69	106149.0
70	78135.5
71	50122.0
72	50122.0
73	50122.0
74	35376.5
75	20631.0
76	20631.0
77	20631.0
78	14101.5
79	7572.0
80	7572.0
81	7572.0
82	5083.5
83	2595.0
84	2595.0
85	2595.0
86	1665.0
87	735.0
88	735.0
89	735.0
90	474.5
91	214.0
92	214.0
93	214.0
94	130.5
95	47.0
96	47.0
97	47.0
98	32.5
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.313950759281152
2	0.18445941764289375
3	0.20201756566164067
4	0.2231545704274519
5	0.2503222454024049
6	0.19948666144272217
7	0.19487962485672217
8	0.21900230569328882
9	0.21931866872065361
10	0.22993660282658504
11	0.20294688205452477
12	0.20974868714286818
13	0.22149366453378666
14	0.211053684630748
15	0.2002577963219239
16	0.2039552892042501
17	0.2037575623121471
18	0.2074550551944733
19	0.20664437493685098
20	0.20322369970346899
21	0.20029734170034452
22	0.20361915348767498
23	0.20751437326210423
24	0.22477593094269657
25	0.2181125346788253
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5057481.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.90328921254708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.38628627020786	61.52037833529755
2	11.406898451806711	16.632008337004407
3	2.4972994561247823	5.461840335006047
4	0.7823982481167316	2.28157623047377
5	0.30401041789850625	1.108167970984105
6	0.14794030603381436	0.6471200950185536
7	0.10230556116014354	0.5220888339316909
8	0.064892101999587	0.3784678143748797
9	0.043532766384573124	0.2856313672161104
>10	0.23559122870992813	3.1161865583665467
>50	0.016028938279264816	0.8126836924203774
>100	0.010337531146883313	1.4124906827853494
>500	0.0010078341771433785	0.47974799806285656
>1k	0.001089546632595653	1.9447222289531578
>5k	2.4514799233077634E-4	1.3327221230139903
>10k+	1.3619332907265351E-4	2.064167397090508
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	33835	0.6690089394305189	No Hit
CCCATGTACTCTGCGTTGATACCAC	31318	0.6192410806881924	No Hit
GAGTACATGGGAAGCAGTGGTATCA	15956	0.31549302903955545	No Hit
CATGTACTCTGCGTTGATACCACTG	12564	0.24842406723821603	No Hit
GCGTTGATACCACTGCTTCCCATGT	10274	0.20314460894662778	No Hit
GTATCAACGCAGAGTACATGGGAAG	9113	0.18018851677346884	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	8615	0.17034171754673919	No Hit
GCTTCCCATGTACTCTGCGTTGATA	8508	0.16822603980123702	No Hit
TATCAACGCAGAGTACATGGGAAGC	7818	0.15458288424612965	No Hit
GGTATCAACGCAGAGTACATGGGAA	7514	0.14857198672619829	No Hit
ACTCTGCGTTGATACCACTGCTTCC	7370	0.145724719479915	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6745	0.13336678872347715	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	6116	0.12092976721019813	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5314	0.10507207046353709	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.9772689210300542E-5	0.0
3	0.0	0.0	0.0	3.9545378420601083E-5	0.0
4	0.0	0.0	0.0	3.9545378420601083E-5	0.0
5	0.0	0.0	0.0	3.9545378420601083E-5	0.0
6	1.9772689210300542E-5	0.0	0.0	3.9545378420601083E-5	0.0
7	1.9772689210300542E-5	0.0	0.0	3.9545378420601083E-5	0.0
8	1.9772689210300542E-5	0.0	0.0	3.9545378420601083E-5	0.0
9	3.9545378420601083E-5	0.0	0.0	3.9545378420601083E-5	0.0
10	3.9545378420601083E-5	0.0	0.0	3.9545378420601083E-5	0.0
11	3.9545378420601083E-5	0.0	0.0	3.9545378420601083E-5	0.0
12	3.9545378420601083E-5	0.0	0.0	3.9545378420601083E-5	0.0
13	3.9545378420601083E-5	0.0	0.0	3.9545378420601083E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGCGT	70	1.09265166E-4	12.213311	8
GTACGGA	80	2.8320273E-5	11.890203	1
GACGGCG	80	2.871987E-5	11.873935	7
AGACCGT	100	1.934768E-6	11.39875	6
CTTAACG	60	0.0058575566	11.088715	2
TAGGACG	155	3.6288839E-9	10.426379	4
ACACCGT	185	6.002665E-11	10.269145	6
GCGGTTG	115	1.0310061E-5	9.910287	18
CCGTGCA	135	6.459177E-7	9.851066	9
CTAGGAC	550	0.0	9.850333	3
GTCTACG	90	0.0011014619	9.512162	1
TACACCG	100	2.741177E-4	9.5056505	5
AGGACCG	130	4.2117445E-6	9.50565	5
CGGCGTG	90	0.001115241	9.499242	9
CACTCGT	80	0.0045232414	9.498771	10
CCTACAC	545	0.0	9.417508	3
TCTGTCG	280	0.0	9.159983	8
GTGTTAG	765	0.0	9.076965	1
CTAGGCG	95	0.0018137792	9.006068	4
ACTCCGC	95	0.0018262625	8.999281	8
>>END_MODULE