FastQCFastQC Report
Wed 25 May 2016
SRR1295052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295052_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5057481
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC340970.6741893840036176No Hit
GTACATGGGAAGCAGTGGTATCAAC329350.6512135191412484No Hit
GAGTACATGGGAAGCAGTGGTATCA169790.3357204901016929No Hit
GTATCAACGCAGAGTACTTTTTTTT167320.33083663586674866No Hit
GGTATCAACGCAGAGTACTTTTTTT142140.2810490044352119No Hit
CATGTACTCTGCGTTGATACCACTG138010.2728828837913578No Hit
TATCAACGCAGAGTACTTTTTTTTT117660.2326454612483962No Hit
GCGTTGATACCACTGCTTCCCATGT111650.22076207503300554No Hit
ACGCAGAGTACATGGGAAGCAGTGG104750.20711891947789818No Hit
GTATCAACGCAGAGTACATGGGAAG93120.18412328192631866No Hit
GCTTCCCATGTACTCTGCGTTGATA92430.18275896637080793No Hit
TATCAACGCAGAGTACATGGGAAGC85210.16848308476097093No Hit
GGTATCAACGCAGAGTACATGGGAA80130.15843855864213827No Hit
ACTCTGCGTTGATACCACTGCTTCC78350.15491901996270474No Hit
ACGCAGAGTACTTTTTTTTTTTTTT68310.135067239995563No Hit
GTGGTATCAACGCAGAGTACATGGG51260.10135480489200058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCGT500.00150117513.2990458
CTATCGG600.005882339611.08242814
TAGGACT6050.010.0488654
GTCCTAG5200.09.6959921
CGCGATC1502.6554517E-79.49922417
GTCTTAG5050.09.4188611
CCTAGAC4950.09.211463
TAGACAG6750.09.1474915
CTTGCAC3850.09.1292153
CTAGACA5650.09.0789944
TCTGTCG3350.09.0739758
GCGCGAT1502.7080132E-68.86594216
TAGACTG7200.08.8396435
CTATACT6500.08.7686014
GACTGCG1201.7666357E-48.7077087
GTGTAGG6550.08.5690061
CTAAGAC5500.08.4630293
CCTATAC4850.08.4220753
TCAGGAC6700.08.365073
GTCTAAT4450.08.3372781
Produced by FastQC (version 0.11.5)