FastQCFastQC Report
Wed 25 May 2016
SRR1295051_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295051_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5319385
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC201770.3793107661881966No Hit
CCCATGTACTCTGCGTTGATACCAC193800.36432783113085443No Hit
GTATCAACGCAGAGTACTTTTTTTT96560.1815247439318643No Hit
GAGTACATGGGAAGCAGTGGTATCA91490.17199356692549986No Hit
CATGTACTCTGCGTTGATACCACTG71840.13505320633870269No Hit
GGTATCAACGCAGAGTACTTTTTTT71430.1342824405452886No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN64480.12121702038863515No Hit
TATCAACGCAGAGTACTTTTTTTTT63620.11960029213903486No Hit
GCGTTGATACCACTGCTTCCCATGT62390.1172879947587926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC2050.011.5906143
ATCGCTA752.078878E-411.39807210
CGACCAT1850.011.2949616
GTCGTAC1303.213063E-810.9751971
GTACCGT1106.067914E-610.3614936
GGGTACG1752.3101165E-1010.3271381
TCGTACC1651.0168151E-89.7922212
GGTACGA2151.0913936E-119.7252432
GTCTAGA5200.09.5118371
AAGACCG2303.6379788E-129.5043955
GGCGCAA1701.675653E-89.49731915
TACGACC2151.05501385E-109.2844114
CGTACCC1856.65932E-99.2474323
GAACCGT1054.5053012E-49.0457486
TGCGCTA950.0018277798.99847810
TAGACAG6350.08.980535
CTCGCAC850.0074121058.9452283
CGCAATC1606.7761175E-78.90390517
ATCGCTC1201.7681823E-48.7070258
CGCTATC1752.6391172E-78.68359112
Produced by FastQC (version 0.11.5)