FastQCFastQC Report
Wed 25 May 2016
SRR1295051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295051_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5319385
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT270830.5091378044642378No Hit
CCCATGTACTCTGCGTTGATACCAC213110.4006290200840886No Hit
GGTATCAACGCAGAGTACTTTTTTT211000.3966623961228601No Hit
GTACATGGGAAGCAGTGGTATCAAC192280.3614703579455144No Hit
TATCAACGCAGAGTACTTTTTTTTT189400.3560561982259227No Hit
ACGCAGAGTACTTTTTTTTTTTTTT96350.18112996145231075No Hit
GAGTACATGGGAAGCAGTGGTATCA94570.177783709958952No Hit
CATGTACTCTGCGTTGATACCACTG80100.15058131720114262No Hit
GCGTTGATACCACTGCTTCCCATGT68120.1280599167008968No Hit
ACGCAGAGTACATGGGAAGCAGTGG59350.11157304838811254No Hit
GTACTTTTTTTTTTTTTTTTTTTTT56440.10610249117144183No Hit
TATCAACGCAGAGTACATGGGAAGC53670.10089512227447346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC1750.013.0459351
TCGTACC1800.012.6660212
TAGACCG855.3315292E-511.1757965
GGGTACG1855.456968E-1210.7981551
CGTACCC2151.8189894E-1210.1621773
ATCCGTA951.6469946E-49.99911412
CGCAAGT1054.113293E-59.95149912
CCTATAC4450.09.6061613
GTATTAG7350.09.5773731
GTACGAC2002.6557245E-109.4994283
GGCCCGT1606.6531356E-89.4994276
TAGACAG7350.09.4348055
GTATTAT8600.09.2914351
GTGCTAG5350.09.245951
GGTACGA2106.693881E-109.0471582
CGACCAT2106.693881E-109.0470736
GCACCGT1502.707342E-68.8661326
TAGGACG1401.0871581E-58.8208954
TAGACTG6250.08.6634775
TATACTG10200.08.66124255
Produced by FastQC (version 0.11.5)