FastQCFastQC Report
Wed 25 May 2016
SRR1295050_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295050_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3186452
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC168330.5282678038143992No Hit
CCCATGTACTCTGCGTTGATACCAC148030.4645605833698421No Hit
GAGTACATGGGAAGCAGTGGTATCA81260.255017178981513No Hit
CATGTACTCTGCGTTGATACCACTG57380.18007489207431965No Hit
GCTTCCCATGTACTCTGCGTTGATA49740.15609838152277203No Hit
GTATCAACGCAGAGTACTTTTTTTT48470.15211275738658545No Hit
GCGTTGATACCACTGCTTCCCATGT47370.14866064199303802No Hit
GTATCAACGCAGAGTACATGGGAAG43930.1378649356713988No Hit
GGTATCAACGCAGAGTACTTTTTTT43590.13679791818612047No Hit
ACGCAGAGTACATGGGAAGCAGTGG42970.1348521804188483No Hit
GGTATCAACGCAGAGTACATGGGAA41390.12989368739902563No Hit
TATCAACGCAGAGTACATGGGAAGC37670.1182192607953925No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN37620.11806234645932215No Hit
ATTCCATTCCATTCCATTCCATTCC37460.11756022058389706No Hit
ACTCTGCGTTGATACCACTGCTTCC34250.10748632020818139No Hit
TATCAACGCAGAGTACTTTTTTTTT32550.10215123278178989No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC604.0599555E-412.6792551
CCGTGCA751.4816249E-512.6650949
CGTACCC802.8574568E-511.8795053
GAGCCGT658.0379576E-411.6904896
TGTACCG856.57402E-410.0626395
GTCTTGC5650.09.9302131
AGAGCCG900.00111032929.5036045
TGTGCGA800.00452227159.4988210
CAATCCG1106.850028E-59.4988210
CGAATGT1106.855924E-59.49807415
CTGTCGC2252.7284841E-119.2877359
GTCGCCC1951.6916601E-99.25438711
GTGTAGG3700.09.2524291
TGTCGCC2201.6916601E-109.06705610
CTAGTAC2106.6393113E-109.0510523
GTCCTAG3600.08.9811381
GTGTAGA4450.08.9752031
TACACTG6950.08.8882635
ATAACAC4950.08.8316323
GCTACAC2950.08.6982143
Produced by FastQC (version 0.11.5)