FastQCFastQC Report
Wed 25 May 2016
SRR1295050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3186452
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC169400.5316257706063044No Hit
CCCATGTACTCTGCGTTGATACCAC164470.5161540170697692No Hit
GTATCAACGCAGAGTACTTTTTTTT127560.4003198541826458No Hit
GGTATCAACGCAGAGTACTTTTTTT120760.37897950447707984No Hit
TATCAACGCAGAGTACTTTTTTTTT92570.2905112018006234No Hit
GAGTACATGGGAAGCAGTGGTATCA91250.28636866332836647No Hit
CATGTACTCTGCGTTGATACCACTG66310.20809979249648197No Hit
GCTTCCCATGTACTCTGCGTTGATA55210.173264809888867No Hit
ACGCAGAGTACATGGGAAGCAGTGG53390.1675531280559067No Hit
GCGTTGATACCACTGCTTCCCATGT52150.16366165252136233No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51630.1620297434262308No Hit
GTATCAACGCAGAGTACATGGGAAG46360.1454909724044172No Hit
GGTATCAACGCAGAGTACATGGGAA44320.13908886749274743No Hit
TATCAACGCAGAGTACATGGGAAGC43110.13529154055984524No Hit
ATTCCATTCCATTCCATTCCATTCC37610.11803096359210809No Hit
ACTCTGCGTTGATACCACTGCTTCC37380.11730915764618453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGACG550.00307152412.0899314
GCACCGT1002.4026898E-510.4491546
TACACCG1106.056349E-610.3627985
CTGTCGC2600.010.2301029
GACCGTG1054.1108353E-59.9517317
TAGACAG5150.09.5914565
TCTGTCG2500.09.499388
GTCTTAC3350.09.3706861
TGTCGCC2501.8189894E-129.11940510
ATTACAC3450.09.0862213
ATACCGT850.00744327628.9404536
TAATACT5550.08.9001814
CTAATAC4400.08.8515573
CTAGACT3550.08.8302724
GTTCTAT3350.08.8027651
CTAGAAC3700.08.7290233
TCTAGAC2850.08.6659653
TGCACTG5400.08.6196735
GTACTAG2553.8198777E-118.580051
AGCCCCG1000.00290826878.5493085
Produced by FastQC (version 0.11.5)