FastQCFastQC Report
Wed 25 May 2016
SRR1295049_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295049_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784385
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71560.25700468864758286No Hit
GGTATCAACGCAGAGTACTTTTTTT64710.2324032057348391No Hit
TATCAACGCAGAGTACTTTTTTTTT48010.1724258678307777No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA40440.14523853561917624No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN33940.1218940627822661No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACC456.7343924E-414.7865324
GGTATCA16000.014.5630981
CGTGCGT602.5732346E-514.24792210
TCGTCGT500.001501308613.2985388
GACGCGT751.4817961E-512.664826
CGCGTGC853.9513216E-612.2927688
GTCGTCG550.00307243212.0893637
ACGCGTG802.8720213E-511.8734827
TTGACGC1459.822543E-1111.78989817
CGTCGTA658.035526E-411.6908139
AGACGCG951.3527877E-511.006125
TTTGACG1752.382876E-1010.311110516
TGACCCG1303.8153667E-710.2364625
TGGACGG2002.5465852E-119.980555
GCGTGCG1054.113147E-59.9511089
GTACTAG1356.350092E-79.8628391
TCTGGAC3000.09.8214213
GTCCAGG6750.09.7219411
TACACTG5150.09.5975695
GTCCCGC1106.756994E-59.5105951
Produced by FastQC (version 0.11.5)