FastQCFastQC Report
Wed 25 May 2016
SRR1295049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295049_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784385
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT211620.7600242064226032No Hit
GGTATCAACGCAGAGTACTTTTTTT197660.7098874616836393No Hit
TATCAACGCAGAGTACTTTTTTTTT152610.5480923076370545No Hit
ACGCAGAGTACTTTTTTTTTTTTTT86640.31116386562921433No Hit
GTACTTTTTTTTTTTTTTTTTTTTT37900.13611623392598365No Hit
GAGTACTTTTTTTTTTTTTTTTTTT36640.1315909976529826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33950.015.2978551
CGTAATT707.2742314E-613.5702116
GTGGTAT6250.012.1755251
TGTGCGC550.003070733312.0902598
TGTACCG550.003071146312.090045
GTCGTAT802.8310611E-511.89016151
GTCGTAC657.941463E-411.7072351
TCGTCCG658.031763E-411.6914679
TCGTATC907.467881E-611.6109032
CTAGTAC1251.835906E-811.3991813
GCTCGTT1251.835906E-811.39918111
TTCGTCT1201.27784E-711.08253818
TTTCGTC1053.47256E-610.85616817
ACGTAAT1002.4026245E-510.44906115
TGGTATC7450.010.20115952
AGGACCG2250.010.1326065
ACCGTCA750.00265027610.13260612
AGACCGT1901.0004442E-109.9992836
GTATTAG2500.09.5121291
GGCCTAG1701.6319973E-89.5121291
Produced by FastQC (version 0.11.5)