FastQCFastQC Report
Wed 25 May 2016
SRR1295048_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295048_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3761294
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC361660.9615307923283849No Hit
CCCATGTACTCTGCGTTGATACCAC328110.8723327663298854No Hit
GAGTACATGGGAAGCAGTGGTATCA170820.4541522146367713No Hit
CATGTACTCTGCGTTGATACCACTG126940.33749023607301104No Hit
GCGTTGATACCACTGCTTCCCATGT104840.2787338612722111No Hit
GTATCAACGCAGAGTACATGGGAAG92150.244995472302883No Hit
GCTTCCCATGTACTCTGCGTTGATA91110.24223046642990417No Hit
ACGCAGAGTACATGGGAAGCAGTGG88220.23454694049441496No Hit
TATCAACGCAGAGTACATGGGAAGC80370.21367646347241137No Hit
GGTATCAACGCAGAGTACATGGGAA79620.2116824688524747No Hit
GTATCAACGCAGAGTACTTTTTTTT75360.20035657941123455No Hit
ACTCTGCGTTGATACCACTGCTTCC73970.1966610427156186No Hit
GGTATCAACGCAGAGTACTTTTTTT62820.16701698936589376No Hit
ATTCCATTCCATTCCATTCCATTCC56040.14899127800166645No Hit
GTGGTATCAACGCAGAGTACATGGG53940.14340809306584382No Hit
TATCAACGCAGAGTACTTTTTTTTT50960.1354852877759622No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN44700.11884207934822431No Hit
CAGTGGTATCAACGCAGAGTACATG40460.10756936309684911No Hit
GAATGGAATGGAATGGAATGGAATG40270.10706421779313183No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG657.993554E-411.6983145
ATAGACG700.00148752810.8612733
GAACCGC700.001493494310.8560626
GTATACG856.543996E-410.0677491
TCGTCTA856.6125236E-410.05634814
CGTCTAT856.6141435E-410.0560815
TAGACAG6000.09.504885
ATTAGAC2303.6379788E-129.5036133
CAGTGCG800.00451968759.499569
GTAGAAC2800.09.1641993
TAGACTG4950.09.0248365
ACCGCGC950.00182946688.99742518
GAACGAT850.0074538848.93897516
GTCTAAG3450.08.8194131
GTGTTAG5500.08.8169081
TTAGACA3800.08.7547284
GTACTAG3050.08.7290511
GTATAGG4700.08.6992021
AGAACCG1107.111841E-48.64085
CGCGATC1107.166744E-48.63412317
Produced by FastQC (version 0.11.5)