FastQCFastQC Report
Wed 25 May 2016
SRR1295048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295048_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3761294
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC365470.971660284997663No Hit
CCCATGTACTCTGCGTTGATACCAC354570.9426808965212504No Hit
GTATCAACGCAGAGTACTTTTTTTT212130.5639814383028819No Hit
GAGTACATGGGAAGCAGTGGTATCA192520.5118451256402716No Hit
GGTATCAACGCAGAGTACTTTTTTT186940.497009805667943No Hit
TATCAACGCAGAGTACTTTTTTTTT151840.4036908574549078No Hit
CATGTACTCTGCGTTGATACCACTG141110.37516344109234745No Hit
ACGCAGAGTACATGGGAAGCAGTGG113540.30186419886347626No Hit
GCGTTGATACCACTGCTTCCCATGT112060.2979293828134679No Hit
GCTTCCCATGTACTCTGCGTTGATA97650.25961809951575177No Hit
GTATCAACGCAGAGTACATGGGAAG95600.2541678475545916No Hit
GGTATCAACGCAGAGTACATGGGAA90440.24044916456942744No Hit
TATCAACGCAGAGTACATGGGAAGC88970.2365409351143516No Hit
ACGCAGAGTACTTTTTTTTTTTTTT84860.22561384459709877No Hit
ACTCTGCGTTGATACCACTGCTTCC81380.21636170956059272No Hit
GTGGTATCAACGCAGAGTACATGGG58280.1549466752665439No Hit
ATTCCATTCCATTCCATTCCATTCC53680.14271684159759912No Hit
CAGTGGTATCAACGCAGAGTACATG49520.13165681810568386No Hit
ACATGGGAAGCAGTGGTATCAACGC47630.1266319516634435No Hit
GTACTTTTTTTTTTTTTTTTTTTTT44740.11894842572795428No Hit
CATGGGAAGCAGTGGTATCAACGCA44070.11716712386747752No Hit
GAATGGAATGGAATGGAATGGAATG42780.1137374531211865No Hit
GGGAAGCAGTGGTATCAACGCAGAG41170.10945701133705582No Hit
GCAGTGGTATCAACGCAGAGTACAT41030.10908479900800097No Hit
GAGTACTTTTTTTTTTTTTTTTTTT39560.10517656955292515No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCT402.7632652E-416.6243384
CTGTCCG658.032548E-411.691539
CTACGCT600.005880034511.0828914
CTAGACA3550.010.9713944
TAGCCCG803.7747482E-410.6870745
CGTGCAC909.52882E-510.55485410
GGAACGA1909.094947E-1210.49930215
CTTGGAC2950.010.3046743
GAACGAA1201.5266924E-610.29098216
GTCTAGG3050.09.6658891
GCCCTAA1804.114554E-99.5099891
TAGACAG5400.09.4996215
CCTACAC4100.09.4996213
TACGCTG800.0045194529.4996215
ACCGTTT800.00451994739.4994958
TCGGAAC1201.7032173E-59.49936913
GTCCTAC2600.09.1442191
GTCTAGA3250.09.0710651
TCGCGTT1054.4982097E-49.04701810
AACCGAG950.00182559548.9995217
Produced by FastQC (version 0.11.5)