FastQCFastQC Report
Wed 25 May 2016
SRR1295047_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295047_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4933827
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC361450.7325956098582298No Hit
CCCATGTACTCTGCGTTGATACCAC335190.6793712061651128No Hit
GAGTACATGGGAAGCAGTGGTATCA161320.3269672811794982No Hit
CATGTACTCTGCGTTGATACCACTG126030.25544065489122336No Hit
GCGTTGATACCACTGCTTCCCATGT111040.22505856001841978No Hit
GTATCAACGCAGAGTACATGGGAAG92020.1865083635887517No Hit
TATCAACGCAGAGTACATGGGAAGC83820.16988840508595054No Hit
ACGCAGAGTACATGGGAAGCAGTGG83040.16830748220397676No Hit
ACTCTGCGTTGATACCACTGCTTCC74760.1515253777645629No Hit
GGTATCAACGCAGAGTACATGGGAA73720.14941748058859786No Hit
GTATCAACGCAGAGTACTTTTTTTT72690.1473298516547094No Hit
GCTTCCCATGTACTCTGCGTTGATA69990.14185742629403097No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN59360.12031228496661922No Hit
GGTATCAACGCAGAGTACTTTTTTT56280.11406966640703049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCCG1950.013.15183510
GACCCGT1750.013.0259186
GGTCCGC2350.010.91200511
CGACGTA700.001494049710.8557029
ACGTGCG700.001494049710.8557029
CGCACGA803.7826915E-410.68477912
ACCCGTG2250.010.5541557
TATACCG1303.822097E-710.2356525
GTCTTAA4350.010.0574181
GTATACG1151.0137817E-59.9243341
GTGCTAG3750.09.8912521
GTCCTAC3200.09.8080321
TAGGACT6250.09.7331374
GTATTAG5700.09.6776761
TAGGCCG1201.6926304E-59.5045345
GAACCGT1002.763212E-49.4980656
GTCCGCT2600.09.49758212
GGTCTAG3750.09.3840081
ACGCAAA2750.09.3378951
ACCGTCC1054.5013556E-49.0465118
Produced by FastQC (version 0.11.5)