##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295047_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4933827
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32103719891273	33.0	33.0	33.0	27.0	33.0
2	31.17939887231555	33.0	33.0	33.0	27.0	33.0
3	31.306578240380134	33.0	33.0	33.0	27.0	33.0
4	34.89978590655895	37.0	37.0	37.0	33.0	37.0
5	35.13039391125793	37.0	37.0	37.0	33.0	37.0
6	35.15526122014412	37.0	37.0	37.0	33.0	37.0
7	34.999499982467974	37.0	37.0	37.0	33.0	37.0
8	34.94437847131648	37.0	37.0	37.0	33.0	37.0
9	34.84569665697642	37.0	37.0	37.0	33.0	37.0
10	34.74607601766337	37.0	37.0	37.0	27.0	37.0
11	34.95310982732066	37.0	37.0	37.0	33.0	37.0
12	34.86952238090229	37.0	37.0	37.0	33.0	37.0
13	34.790665339502176	37.0	37.0	37.0	33.0	37.0
14	36.179433733691916	40.0	37.0	40.0	27.0	40.0
15	36.352822058819655	40.0	37.0	40.0	27.0	40.0
16	36.41405910665291	40.0	37.0	40.0	33.0	40.0
17	36.33729253174057	40.0	37.0	40.0	27.0	40.0
18	36.25376183640002	40.0	37.0	40.0	27.0	40.0
19	36.34290926698484	40.0	37.0	40.0	27.0	40.0
20	36.36253662724696	40.0	37.0	40.0	27.0	40.0
21	36.35011847800906	40.0	37.0	40.0	27.0	40.0
22	36.25664762870689	40.0	37.0	40.0	27.0	40.0
23	36.146204761537035	40.0	37.0	40.0	27.0	40.0
24	36.219267112527454	40.0	37.0	40.0	27.0	40.0
25	35.0997596794537	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.42967400487190943
1101	2	0.3681130281337701
1101	3	0.4079287790934707
1101	4	0.512729767961055
1101	5	0.3333626590385208
1101	6	0.27614555613805436
1101	7	0.29771815281355174
1101	8	0.46852193544904
1101	9	0.46409119565578294
1101	10	0.6755764007192866
1101	11	0.49798082898830387
1101	12	0.5184555880443256
1101	13	0.489642914547062
1101	14	1.0216359836668119
1101	15	0.8667959911905001
1101	16	0.8571941841567536
1101	17	0.8664260025302752
1101	18	0.8424540408851158
1101	19	0.7843599999206603
1101	20	0.7383644820171043
1101	21	0.7749422143415501
1101	22	0.8040569300784881
1101	23	0.8935977391158048
1101	24	0.8815986409221779
1101	25	0.9541502218289466
1102	1	0.3411140516236131
1102	2	0.3536525562063666
1102	3	0.3838888803234113
1102	4	0.4590217135769308
1102	5	0.2748565190864838
1102	6	0.19247737101240148
1102	7	0.31156421511146704
1102	8	0.35023242465037896
1102	9	0.3387090550185121
1102	10	0.564719218909822
1102	11	0.3455095069819407
1102	12	0.42337173481710266
1102	13	0.3406066629107727
1102	14	0.8113752555089775
1102	15	0.7378250895012073
1102	16	0.7642365402308187
1102	17	0.619022882575976
1102	18	0.7971018905592047
1102	19	0.6949413859385416
1102	20	0.6716438631688462
1102	21	0.7413365852261222
1102	22	0.7237560204489384
1102	23	0.7100736701328856
1102	24	0.6914789372526826
1102	25	0.7932815903424455
1103	1	0.4276437132725235
1103	2	0.31603102180031684
1103	3	0.3783453785902573
1103	4	0.45504524707882865
1103	5	0.2946045939158495
1103	6	0.21083821349120768
1103	7	0.32197958536998783
1103	8	0.44252351166982606
1103	9	0.4704481506316043
1103	10	0.5515993627608466
1103	11	0.4085156144348332
1103	12	0.4703746594412692
1103	13	0.3967561902206995
1103	14	0.8836964412226891
1103	15	0.7853034162194419
1103	16	0.7101164528885491
1103	17	0.7129130081101565
1103	18	0.8552313553162421
1103	19	0.7414486863168577
1103	20	0.748308600078289
1103	21	0.6658747032924097
1103	22	0.7754102396611344
1103	23	0.7931342888101938
1103	24	0.7997475391272317
1103	25	0.9498372960897328
1104	1	0.35405099865458567
1104	2	0.2666138232744828
1104	3	0.3237471521069537
1104	4	0.38977373508910773
1104	5	0.25295515836440785
1104	6	0.19233142533495595
1104	7	0.21815218153672333
1104	8	0.4052058358824979
1104	9	0.3213065738947236
1104	10	0.5005180663116988
1104	11	0.29527232116515023
1104	12	0.43245513050811013
1104	13	0.34966369492514815
1104	14	0.6927695289111426
1104	15	0.5601627817987733
1104	16	0.4325717427791318
1104	17	0.5506866691170345
1104	18	0.6806745491065342
1104	19	0.5558960281238114
1104	20	0.6326751491075342
1104	21	0.4578916250556375
1104	22	0.6214288823493135
1104	23	0.706757774159513
1104	24	0.6672562820762593
1104	25	0.7917582607431797
1105	1	0.2594461187629875
1105	2	0.1867522650276925
1105	3	0.1931653081390401
1105	4	0.3419048771188997
1105	5	0.27787612693531116
1105	6	0.25574802182110545
1105	7	0.1923637618348195
1105	8	0.28552151145591864
1105	9	0.30806147333050404
1105	10	0.40081688431605045
1105	11	0.21715214666922122
1105	12	0.3418023206473535
1105	13	0.2937835742640402
1105	14	0.6878361899557746
1105	15	0.538453070448675
1105	16	0.4587522569449547
1105	17	0.46936307216706297
1105	18	0.40825935262198243
1105	19	0.44948671882545455
1105	20	0.4122123622553602
1105	21	0.48353154031480017
1105	22	0.4158150440429438
1105	23	0.5402417724318767
1105	24	0.5562387054180533
1105	25	0.43856006621532373
1106	1	0.0841633991083981
1106	2	-0.13396009763678407
1106	3	-0.0989589412448808
1106	4	-0.0018855519679519261
1106	5	-0.2557349062790095
1106	6	-0.2516381344135681
1106	7	-0.6924534857678282
1106	8	-0.2369066933790407
1106	9	0.011406855426010054
1106	10	-0.2777181003073821
1106	11	-0.3414641739529216
1106	12	-0.7752623768580804
1106	13	-0.20333200768764215
1106	14	-0.2462794969607529
1106	15	-0.1465259130843748
1106	16	-0.05375724080613509
1106	17	-0.1542854663934108
1106	18	-0.3209472894588927
1106	19	-0.4586637420015123
1106	20	-0.04815130957129554
1106	21	0.1082229515278641
1106	22	0.27074496434799755
1106	23	-0.43411661042031824
1106	24	0.12393176238575876
1106	25	0.3736325585348723
1107	1	-1.0924077873819655
1107	2	-1.080197013675317
1107	3	-0.8542801383735714
1107	4	-0.9511237058350517
1107	5	-0.982476069610918
1107	6	-0.8663355756356239
1107	7	-1.0538662368205536
1107	8	-0.9803398189576455
1107	9	-0.9317815413259396
1107	10	-0.8749973360877235
1107	11	-0.8077594920483833
1107	12	-0.8476605964275024
1107	13	-0.7062615668258729
1107	14	-1.1904586293838264
1107	15	-1.0059129373501676
1107	16	-1.0058893035921272
1107	17	-0.9695202141042785
1107	18	-1.2403823327734358
1107	19	-1.1628279145712668
1107	20	-1.1892095820770336
1107	21	-1.119116416810769
1107	22	-1.1479849233017987
1107	23	-0.8811142851182083
1107	24	-0.7340931776377104
1107	25	-0.808255806160254
1108	1	0.03582276766493564
1108	2	-0.195929208317704
1108	3	-0.024899756117306993
1108	4	-0.16254912144815847
1108	5	-0.4207892602702188
1108	6	-0.03359002916656806
1108	7	0.023240237536178654
1108	8	0.2775736480627842
1108	9	-0.178477155781394
1108	10	-0.5071131480219009
1108	11	-0.034791519029958806
1108	12	0.12784579507283667
1108	13	0.20059681238366522
1108	14	-0.2818822138447459
1108	15	0.05741369474538516
1108	16	-0.8102914713646285
1108	17	-0.7089248883475037
1108	18	-0.6275142354704215
1108	19	0.014195833264793123
1108	20	-0.8712137391821031
1108	21	-0.1655288195551634
1108	22	-0.2901456114513863
1108	23	0.03996906217603424
1108	24	-0.4058119412333667
1108	25	-1.18219019896447
1109	1	0.18489981238485598
1109	2	0.10544299091073484
1109	3	0.19964004816894843
1109	4	0.1887885132490581
1109	5	0.1343565184889215
1109	6	0.10788209067739984
1109	7	0.2037549261958418
1109	8	0.23584555444227817
1109	9	0.27407859376882726
1109	10	0.34501852131834454
1109	11	0.18100694063272726
1109	12	0.2847655382571048
1109	13	0.2831914818492578
1109	14	0.5632989460801525
1109	15	0.4391458335555285
1109	16	0.3004191019634561
1109	17	0.42289116124310766
1109	18	0.44445769635768784
1109	19	0.4046489008352836
1109	20	0.40876399644861294
1109	21	0.4922287336811806
1109	22	0.4527202629742746
1109	23	0.5293265733778583
1109	24	0.5788943931917672
1109	25	0.5687843815449298
1110	1	0.07568707246394268
1110	2	0.03718300261801133
1110	3	0.14572726768471256
1110	4	-0.959520268788296
1110	5	-0.15731733998194386
1110	6	0.04641377887713105
1110	7	0.09804222175622357
1110	8	0.19635020063185493
1110	9	0.1402605250851039
1110	10	0.0566627366442205
1110	11	0.16770213900721842
1110	12	0.20275129801995462
1110	13	0.18541821937107983
1110	14	0.3453742327271314
1110	15	0.11924811263182278
1110	16	0.16651572324609276
1110	17	0.24871769709485392
1110	18	0.307057411949593
1110	19	0.2024988276880535
1110	20	0.20098466961388084
1110	21	0.2693807668210155
1110	22	-0.29982768092376233
1110	23	0.12249529418907201
1110	24	0.3161905888228915
1110	25	0.39760451558053234
1111	1	-0.050795401469905244
1111	2	-0.3066886339780126
1111	3	-0.16045045659600632
1111	4	-0.14324768362678242
1111	5	-0.10623138522291242
1111	6	-0.062408528711834776
1111	7	-0.08596406826691805
1111	8	0.07006825816052498
1111	9	0.018570199253815645
1111	10	-0.05002390003937052
1111	11	-0.0935805172830868
1111	12	-0.048607417895496496
1111	13	-0.11172169639024077
1111	14	-0.23565597979290231
1111	15	-0.13563412146874754
1111	16	-0.19080352661709554
1111	17	-0.13591838734284778
1111	18	-0.1111283194803363
1111	19	-0.15627346306567347
1111	20	-0.17711983187653146
1111	21	-0.2522426883735207
1111	22	-0.09948861226477135
1111	23	-0.05716709635017736
1111	24	0.009389858679199392
1111	25	-0.06193020934605897
1112	1	-0.05753670193057303
1112	2	-0.37964674955335553
1112	3	-0.03315274259248824
1112	4	-0.06426335776167491
1112	5	-0.03675109663023335
1112	6	-0.03721720765540226
1112	7	0.049619026267855304
1112	8	0.07710269602885944
1112	9	0.04304647704382347
1112	10	0.25486470758936974
1112	11	0.06503336820235717
1112	12	0.04614011448290967
1112	13	0.05926920435820904
1112	14	-0.19673790432651828
1112	15	-0.16442986471771093
1112	16	-0.037910049802313495
1112	17	-0.18845879219673378
1112	18	-0.07596293128926135
1112	19	0.04357794039317753
1112	20	-0.8118937145702247
1112	21	-1.2227547130735488
1112	22	-1.6935778714406098
1112	23	-0.47904395249751985
1112	24	-0.2729792638094608
1112	25	-1.455048204805891
1113	1	-0.2142513568504114
1113	2	-0.1112528211268149
1113	3	-0.1972747105307313
1113	4	-0.5005679065770039
1113	5	-0.2538228011192558
1113	6	-0.1491925231105924
1113	7	-0.0866295899316043
1113	8	0.014142903585799615
1113	9	-0.07205535368593985
1113	10	0.017854953728324574
1113	11	-0.07144065607234751
1113	12	-0.09068970711086877
1113	13	-0.19351644098084364
1113	14	-0.4232045556654995
1113	15	-0.446290554368332
1113	16	-0.46053754004741165
1113	17	-0.4191012453944225
1113	18	-0.8977735773830915
1113	19	-1.0206887696897127
1113	20	-1.2462504308171987
1113	21	-1.0633686829288536
1113	22	-0.4416315361942509
1113	23	-2.0714907361459822
1113	24	-1.1371261392406993
1113	25	-0.6802366473624417
1114	1	-0.26323480323419446
1114	2	-0.3548650422112587
1114	3	-0.32375834332096787
1114	4	-0.5035759015168679
1114	5	-0.5427126841089986
1114	6	-0.40235461204821377
1114	7	-0.4227409867923342
1114	8	-0.12855955159503907
1114	9	-1.164575413610649
1114	10	-0.3228555180745474
1114	11	-0.40263274434384755
1114	12	-0.4050148567088314
1114	13	-0.4135321189986527
1114	14	-0.7567372646084678
1114	15	-1.2876699513172412
1114	16	-1.1361731830581476
1114	17	-1.5440693244177481
1114	18	-1.7115573502281194
1114	19	-1.5952942354523003
1114	20	-0.849849945546076
1114	21	-0.7526541571619418
1114	22	-0.5668522577022799
1114	23	-0.37641675416314513
1114	24	-0.875082324628579
1114	25	-0.5714462397725271
1115	1	-0.8709353189829585
1115	2	-0.6738296375178336
1115	3	-0.7545936881623838
1115	4	-0.8279181271572114
1115	5	-0.6433241204889484
1115	6	-0.6836266405278906
1115	7	-0.5677578431409458
1115	8	-0.42224174444277907
1115	9	-0.8152716495536794
1115	10	-0.5158611705797398
1115	11	-0.6973012076770573
1115	12	-0.7479204086063547
1115	13	-0.6036640238984958
1115	14	-0.7403281926716758
1115	15	-1.769004075178934
1115	16	-1.1835048394535406
1115	17	-0.9897380009637473
1115	18	-2.562115867550446
1115	19	-1.431345586237363
1115	20	-1.2760169505935508
1115	21	-1.1125169173327123
1115	22	-1.0492460687908292
1115	23	-0.7422599671034718
1115	24	-0.6555789598431332
1115	25	-1.0162602981227664
1116	1	-2.1210556237221105
1116	2	-1.315786026187407
1116	3	-2.2415337487041036
1116	4	-1.6571109272068938
1116	5	-1.5164541728197847
1116	6	-1.0258260043568228
1116	7	-1.8847174599794698
1116	8	-3.7829628138883358
1116	9	-2.3548955259783853
1116	10	-1.4876744256878922
1116	11	-1.17646251459756
1116	12	-1.2010576959784487
1116	13	-2.3494964331376664
1116	14	-2.4264966961911227
1116	15	-2.273026373694371
1116	16	-1.7517971725769144
1116	17	-2.4145238821848736
1116	18	-3.3360489207477144
1116	19	-2.1538866825531144
1116	20	-1.7147455153052746
1116	21	-1.4309207824612855
1116	22	-1.3738970569062374
1116	23	-1.2783958439382275
1116	24	-1.4552104064472502
1116	25	-1.439841406976619
1201	1	0.39404852539013646
1201	2	0.3739857405074396
1201	3	0.4652219337600805
1201	4	0.5203898446258606
1201	5	0.3693654506516566
1201	6	0.1872978937873171
1201	7	0.1949345119802004
1201	8	0.35436364732476733
1201	9	0.3122369816399484
1201	10	0.5725047114143251
1201	11	0.39521169513332666
1201	12	0.4318927094415912
1201	13	0.41338090544817874
1201	14	0.9287595635185824
1201	15	0.7951302110614051
1201	16	0.737318839616016
1201	17	0.7082029636188949
1201	18	0.6104449812117707
1201	19	0.6144451205965069
1201	20	0.6846205707004884
1201	21	0.6484936402729744
1201	22	0.6892999350356916
1201	23	0.8041166053758388
1201	24	0.7541655527262847
1201	25	0.9190957422439254
1202	1	0.35925896743547625
1202	2	0.3539270320925638
1202	3	0.4598502542499716
1202	4	0.563213841105231
1202	5	0.37069982644169386
1202	6	0.21541650913015786
1202	7	0.1980905340403325
1202	8	0.383208954865097
1202	9	0.3121558011045522
1202	10	0.5434564993524162
1202	11	0.30663764481450784
1202	12	0.3348633365073894
1202	13	0.3008396831520912
1202	14	0.7065752734035016
1202	15	0.5126242887213053
1202	16	0.46659654820879837
1202	17	0.45478542225615115
1202	18	0.5670681705674809
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2211	1	0.26878789894923116
2211	2	0.06462700394587628
2211	3	0.18706566620177867
2211	4	0.19862693259577924
2211	5	0.18747627094523267
2211	6	0.21579218970152425
2211	7	0.16994961410988196
2211	8	0.3125995359128808
2211	9	0.24426973820447984
2211	10	0.38180009270012505
2211	11	0.15912589832118584
2211	12	0.21885277545584358
2211	13	0.1980648860068186
2211	14	0.19150343027897776
2211	15	0.23941121484401862
2211	16	0.16346737908864384
2211	17	0.23456874456680055
2211	18	0.22446358317547066
2211	19	0.20177740854911974
2211	20	0.23624009370669086
2211	21	0.20254978667819756
2211	22	0.1755138253072488
2211	23	0.27795594284146574
2211	24	0.34848610078172726
2211	25	0.2484501551719518
2212	1	0.15229642475705418
2212	2	0.15343648987935055
2212	3	0.18168307945744644
2212	4	0.1716765873109054
2212	5	0.1966621780537139
2212	6	0.24218142269338472
2212	7	0.2604996321238531
2212	8	0.3262998277062579
2212	9	0.1325817130312501
2212	10	0.3908456185363107
2212	11	0.24137484544253596
2212	12	0.2919890332767068
2212	13	0.21982811117405276
2212	14	0.19614703545106948
2212	15	0.24779005959115352
2212	16	0.17805978454131832
2212	17	0.2960476235072207
2212	18	0.36658136660991403
2212	19	0.3727847924786758
2212	20	0.31703873502634394
2212	21	0.376805295423857
2212	22	-0.18525317136446517
2212	23	0.2634287591822613
2212	24	0.30378899111165225
2212	25	0.38786601955526834
2213	1	0.05948178284837269
2213	2	-0.6967796209850441
2213	3	-0.16283732348929902
2213	4	-0.03131754284789423
2213	5	0.04647753300722002
2213	6	0.02459004878813431
2213	7	0.07192230171367697
2213	8	0.19880925483751355
2213	9	-0.1399469460547138
2213	10	-1.5921658525568532
2213	11	-0.38806275443292293
2213	12	-0.11851120449021835
2213	13	-0.07882696093955843
2213	14	0.09926899595214422
2213	15	0.11281003705832404
2213	16	0.06749872845983873
2213	17	-0.019831163112925765
2213	18	0.10587809278278115
2213	19	0.04508676312515547
2213	20	-0.003797640014902015
2213	21	0.05352744201420734
2213	22	0.19901039504284768
2213	23	-0.71942506838257
2213	24	-1.3582073574494657
2213	25	-1.254455381215486
2214	1	-0.07331803202833953
2214	2	-0.2515825699773444
2214	3	-0.07546864271047227
2214	4	-0.15888540172014842
2214	5	-0.07036527921698621
2214	6	-0.22497362037292135
2214	7	-0.11118685110982796
2214	8	-0.07533744692686639
2214	9	-0.4269650712247497
2214	10	-0.0804967439039217
2214	11	-0.13051896655764494
2214	12	-0.12642474563428152
2214	13	-0.16590521765352406
2214	14	-0.09664748760292241
2214	15	-0.25301997784826114
2214	16	-0.3068332039263453
2214	17	-0.23523503966546855
2214	18	-0.25451022030700443
2214	19	-0.8633287167304005
2214	20	-0.5323835240438086
2214	21	-0.4830961838511385
2214	22	-0.7894546439326007
2214	23	-2.0857880567350406
2214	24	-1.5550362242605544
2214	25	-1.8932329811508382
2215	1	-0.5349436707823614
2215	2	-0.28110913434769813
2215	3	-1.3453821116041311
2215	4	-1.1363863957472162
2215	5	-0.4239707114687974
2215	6	-0.4370392095793605
2215	7	-0.3762305138161395
2215	8	-0.2325902479970381
2215	9	-0.4643103702950171
2215	10	-1.5119361897356214
2215	11	-0.6048408087446688
2215	12	-0.7254966858509491
2215	13	-0.6171387612846146
2215	14	-0.9499555348306004
2215	15	-1.1712514168186843
2215	16	-0.830018170147433
2215	17	-0.6642637511652865
2215	18	-0.5585966500433273
2215	19	-1.1448787579007131
2215	20	-1.5656138884123223
2215	21	-0.8737678301981049
2215	22	-0.5844848968885898
2215	23	-0.8224252967161831
2215	24	-2.0320343028218417
2215	25	-0.8769632557612752
2216	1	-0.9318928019205543
2216	2	-0.8207883496404058
2216	3	-1.7465029711359215
2216	4	-1.2157182079563427
2216	5	-0.924713316852575
2216	6	-0.8404286599140107
2216	7	-0.6133606090087369
2216	8	-0.7769000228921144
2216	9	-0.572697361253482
2216	10	-0.6848833813954229
2216	11	-0.7482231499581502
2216	12	-0.6883702171068649
2216	13	-1.381287187609722
2216	14	-1.0952733250468754
2216	15	-1.6021367493561556
2216	16	-1.3542434490974102
2216	17	-2.355624120679636
2216	18	-1.3656032263543452
2216	19	-1.123068173198014
2216	20	-1.0674281276471476
2216	21	-0.8758568690636395
2216	22	-0.9352891496076623
2216	23	-0.7928873699027648
2216	24	-0.9584595692044076
2216	25	-0.9531129357142163
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6567.0
3	670.0
4	853.0
5	721.0
6	550.0
7	348.0
8	526.0
9	1003.0
10	2073.0
11	3878.0
12	6500.0
13	9717.0
14	13572.0
15	17823.0
16	21093.0
17	22546.0
18	23308.0
19	24085.0
20	24622.0
21	26672.0
22	27958.0
23	29957.0
24	32211.0
25	34616.0
26	36610.0
27	41744.0
28	47734.0
29	56844.0
30	69821.0
31	89042.0
32	121153.0
33	173517.0
34	257417.0
35	413570.0
36	762768.0
37	2531738.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.4917155199985	19.600662262293035	12.944226787627333	23.96339543008113
2	15.268886069533746	20.579537221444284	39.339852595738044	24.81172411328393
3	18.64029021569987	25.55815788907154	29.574477850987023	26.227074044241565
4	12.408286125957614	17.6179612223411	36.97869680625776	32.995055845443524
5	12.612447681210101	39.20436223753234	35.135713401839396	13.047476679418171
6	31.971823548884338	37.17829312526275	17.34943551646334	13.500447809389579
7	27.044853702431816	32.883535852540405	22.20478207167868	17.866828373349104
8	25.238212374794916	36.8092531763512	20.294400412104512	17.65813403674937
9	25.189121641138307	17.084953104008065	20.596779782824452	37.12914547202917
10	15.105229916382038	30.11187185716546	33.46199406115623	21.320904165296263
11	32.32022054649599	25.455073136046796	22.821866744764023	19.402839572693196
12	21.755721779851115	27.40032026205918	30.51808196986352	20.325875988226187
13	27.410880238560026	23.062522471647636	25.67957309455663	23.847024195235704
14	21.018021708756724	23.485701391158358	26.970695961024088	28.525580939060834
15	24.031586485286596	29.607049565403532	23.423874433931953	22.93748951537792
16	21.83654009218649	29.969179775839986	26.313017711220084	21.881262420753437
17	21.73661731860765	29.627276702453198	27.31275233648941	21.323353642449742
18	22.063414303233557	28.733468763849235	28.247884964491128	20.955231968426087
19	22.18332364874622	28.97455150477361	28.36012690634209	20.48199794013808
20	22.855964896781224	28.193628249926633	27.757600129169447	21.192806724122697
21	22.255680479621763	28.295165601111314	27.845215561711495	21.603938357555425
22	22.255431294590558	28.122699894875545	26.991454373225928	22.63041443730797
23	21.45063746919894	28.536993361676128	27.729828911439995	22.28254025768493
24	22.072843083921047	29.051630939317203	27.143874577319245	21.731651399442505
25	22.332603432025742	27.555656667425005	26.7465427865709	23.365197113978358
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13010.0
1	13010.0
2	11130.0
3	9250.0
4	9250.0
5	9250.0
6	11798.0
7	14346.0
8	14346.0
9	14346.0
10	18317.5
11	22289.0
12	22289.0
13	22289.0
14	34002.0
15	45715.0
16	45715.0
17	45715.0
18	70398.0
19	95081.0
20	95081.0
21	95081.0
22	131456.0
23	167831.0
24	167831.0
25	167831.0
26	215591.5
27	263352.0
28	263352.0
29	263352.0
30	310669.0
31	357986.0
32	357986.0
33	357986.0
34	394795.5
35	431605.0
36	431605.0
37	431605.0
38	458806.5
39	486008.0
40	486008.0
41	486008.0
42	507469.0
43	528930.0
44	528930.0
45	528930.0
46	591536.5
47	654143.0
48	654143.0
49	654143.0
50	612393.0
51	570643.0
52	570643.0
53	570643.0
54	507437.5
55	444232.0
56	444232.0
57	444232.0
58	390361.5
59	336491.0
60	336491.0
61	336491.0
62	286900.5
63	237310.0
64	237310.0
65	237310.0
66	188572.0
67	139834.0
68	139834.0
69	139834.0
70	103925.5
71	68017.0
72	68017.0
73	68017.0
74	49502.0
75	30987.0
76	30987.0
77	30987.0
78	21091.0
79	11195.0
80	11195.0
81	11195.0
82	7568.0
83	3941.0
84	3941.0
85	3941.0
86	2568.5
87	1196.0
88	1196.0
89	1196.0
90	765.5
91	335.0
92	335.0
93	335.0
94	206.5
95	78.0
96	78.0
97	78.0
98	50.0
99	22.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31484687241769926
2	0.18523146433792673
3	0.203513418691008
4	0.22384246549382458
5	0.24962366941524297
6	0.19978406214891603
7	0.19629792451174313
8	0.21897808739544375
9	0.21899835563752032
10	0.22943650030696253
11	0.20385797880631001
12	0.20995871967136262
13	0.22207912843316152
14	0.2103032797866646
15	0.20164874041996203
16	0.20495246387844568
17	0.2045876355210671
18	0.2081345778844698
19	0.20665499621287897
20	0.20393905177461635
21	0.20160820393580886
22	0.2054997064145135
23	0.20866155217846108
24	0.2245923904506583
25	0.21863352728014174
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4933827.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.70352236892156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.70782425936859	33.463130557360905
2	16.26126337485429	15.839616090482819
3	6.372316410469563	9.31062764517451
4	3.0788940794403357	5.9981194667825015
5	1.7503470320338559	4.262403291401819
6	1.011120041255257	2.9547064528164184
7	0.6288965525491935	2.1440634120372106
8	0.437081456818712	1.7029925207368772
9	0.3197283086324142	1.4014705348310619
>10	1.3158699346774538	11.061527089669369
>50	0.07711970844224765	2.5833009194803727
>100	0.035411173344064385	2.9924309492317573
>500	0.001811120747709333	0.6026243328404791
>1k	0.0017268807641168948	2.0711617542193426
>5k	3.790713872447335E-4	1.365539841677454
>10k+	2.1059521513596303E-4	2.2462851412571543
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	36145	0.7325956098582298	No Hit
CCCATGTACTCTGCGTTGATACCAC	33519	0.6793712061651128	No Hit
GAGTACATGGGAAGCAGTGGTATCA	16132	0.3269672811794982	No Hit
CATGTACTCTGCGTTGATACCACTG	12603	0.25544065489122336	No Hit
GCGTTGATACCACTGCTTCCCATGT	11104	0.22505856001841978	No Hit
GTATCAACGCAGAGTACATGGGAAG	9202	0.1865083635887517	No Hit
TATCAACGCAGAGTACATGGGAAGC	8382	0.16988840508595054	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	8304	0.16830748220397676	No Hit
ACTCTGCGTTGATACCACTGCTTCC	7476	0.1515253777645629	No Hit
GGTATCAACGCAGAGTACATGGGAA	7372	0.14941748058859786	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7269	0.1473298516547094	No Hit
GCTTCCCATGTACTCTGCGTTGATA	6999	0.14185742629403097	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5936	0.12031228496661922	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5628	0.11406966640703049	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.0268242076586796E-5	0.0
5	0.0	2.0268242076586796E-5	0.0	2.0268242076586796E-5	0.0
6	0.0	2.0268242076586796E-5	0.0	2.0268242076586796E-5	0.0
7	0.0	2.0268242076586796E-5	0.0	2.0268242076586796E-5	0.0
8	0.0	2.0268242076586796E-5	0.0	4.053648415317359E-5	0.0
9	0.0	2.0268242076586796E-5	0.0	8.107296830634719E-5	0.0
10	0.0	2.0268242076586796E-5	0.0	1.8241417868928116E-4	0.0
11	0.0	4.053648415317359E-5	0.0	1.8241417868928116E-4	0.0
12	0.0	4.053648415317359E-5	0.0	1.8241417868928116E-4	0.0
13	0.0	4.053648415317359E-5	0.0	1.8241417868928116E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTCCG	195	0.0	13.151835	10
GACCCGT	175	0.0	13.025918	6
GGTCCGC	235	0.0	10.912005	11
CGACGTA	70	0.0014940497	10.855702	9
ACGTGCG	70	0.0014940497	10.855702	9
CGCACGA	80	3.7826915E-4	10.684779	12
ACCCGTG	225	0.0	10.554155	7
TATACCG	130	3.822097E-7	10.235652	5
GTCTTAA	435	0.0	10.057418	1
GTATACG	115	1.0137817E-5	9.924334	1
GTGCTAG	375	0.0	9.891252	1
GTCCTAC	320	0.0	9.808032	1
TAGGACT	625	0.0	9.733137	4
GTATTAG	570	0.0	9.677676	1
TAGGCCG	120	1.6926304E-5	9.504534	5
GAACCGT	100	2.763212E-4	9.498065	6
GTCCGCT	260	0.0	9.497582	12
GGTCTAG	375	0.0	9.384008	1
ACGCAAA	275	0.0	9.337895	1
ACCGTCC	105	4.5013556E-4	9.046511	8
>>END_MODULE