FastQCFastQC Report
Wed 25 May 2016
SRR1295047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295047_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4933827
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC371210.7523774141249784No Hit
GTACATGGGAAGCAGTGGTATCAAC347620.7045646310663102No Hit
GTATCAACGCAGAGTACTTTTTTTT222650.45127240983520495No Hit
GGTATCAACGCAGAGTACTTTTTTT178060.36089631841570446No Hit
GAGTACATGGGAAGCAGTGGTATCA175550.3558089896544812No Hit
TATCAACGCAGAGTACTTTTTTTTT155910.31600216221606475No Hit
CATGTACTCTGCGTTGATACCACTG141770.28734286791977104No Hit
GCGTTGATACCACTGCTTCCCATGT119170.24153664082668483No Hit
ACGCAGAGTACATGGGAAGCAGTGG103390.20955335482983087No Hit
GTATCAACGCAGAGTACATGGGAAG96680.19595336439644115No Hit
TATCAACGCAGAGTACATGGGAAGC94000.19052147551991588No Hit
ACTCTGCGTTGATACCACTGCTTCC85250.1727867637029024No Hit
ACGCAGAGTACTTTTTTTTTTTTTT82580.16737514306845375No Hit
GGTATCAACGCAGAGTACATGGGAA81990.16617931678593514No Hit
GCTTCCCATGTACTCTGCGTTGATA76710.15547768496949732No Hit
GTACTTTTTTTTTTTTTTTTTTTTT52510.10642853914415726No Hit
GTGGTATCAACGCAGAGTACATGGG51530.10444225142065176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGCG852.7052192E-713.41087811
GACCCGT1600.013.0617686
GGTCCGC1657.2759576E-1211.51438911
GACCGTC1104.978265E-711.22687157
GAGCGTA700.001493222210.8564249
CTATGCG803.776116E-410.6867929
AGGTCCG2001.8189894E-1210.44930910
CCGCGAC856.6023704E-410.05815813
GTCCGCT1901.0004442E-109.99933812
CTAGACC3700.09.756213
GTCCTAA4100.09.7428141
GACCGCT1304.2456086E-69.49966057
AGACCGT1106.846988E-59.4994686
CGCACGA800.0045208559.49937212
CTAGTAC2252.7284841E-119.2883683
ACCGTCC1451.7092952E-69.1720878
GTATTGG5100.09.1378681
CGCGACC950.00182635748.99922214
AACGATT850.0074448128.94040417
GTCTTAG3300.08.9344261
Produced by FastQC (version 0.11.5)