FastQCFastQC Report
Wed 25 May 2016
SRR1295046_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295046_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9604
Sequences flagged as poor quality0
Sequence length25
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA830.8642232403165349No Hit
CCCATGTACTCTGCGTTGATACCAC610.6351520199916701No Hit
GTACATGGGAAGCAGTGGTATCAAC210.21865889212827988No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA180.1874219075385256No Hit
GCGTTGATACCACTGCTTCCCATGT170.17700957934194084No Hit
CATGTACTCTGCGTTGATACCACTG170.17700957934194084No Hit
ACTCTGCGTTGATACCACTGCTTCC140.1457725947521866No Hit
GAGTACATGGGAAGCAGTGGTATCA130.13536026655560182No Hit
ACGCAGAGTACATGGGAAGCAGTGG130.13536026655560182No Hit
GTATCAACGCAGAGTACATGGGAAG100.10412328196584757No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)