FastQCFastQC Report
Wed 25 May 2016
SRR1295046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295046_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9604
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC640.6663890045814245No Hit
CCCATGTACTCTGCGTTGATACCAC630.6559766763848397No Hit
GTATCAACGCAGAGTACTTTTTTTT460.4789670970428988No Hit
GGTATCAACGCAGAGTACTTTTTTT430.44773011245314454No Hit
CATGTACTCTGCGTTGATACCACTG320.33319450229071224No Hit
GCGTTGATACCACTGCTTCCCATGT260.27072053311120364No Hit
GAGTACATGGGAAGCAGTGGTATCA260.27072053311120364No Hit
TATCAACGCAGAGTACTTTTTTTTT240.24989587671803418No Hit
TATCAACGCAGAGTACATGGGAAGC230.2394835485214494No Hit
GGTATCAACGCAGAGTACATGGGAA200.20824656393169513No Hit
GTATCAACGCAGAGTACATGGGAAG170.17700957934194084No Hit
ACGCAGAGTACTTTTTTTTTTTTTT170.17700957934194084No Hit
ACGCAGAGTACATGGGAAGCAGTGG150.15618492294877134No Hit
ACTCTGCGTTGATACCACTGCTTCC140.1457725947521866No Hit
GCTTCCCATGTACTCTGCGTTGATA140.1457725947521866No Hit
GCAGTGGTATCAACGCAGAGTACAT120.12494793835901709No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)