##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295046_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9604 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.399521032902957 33.0 33.0 33.0 27.0 33.0 2 31.454810495626823 33.0 33.0 33.0 27.0 33.0 3 31.595689296126615 33.0 33.0 33.0 27.0 33.0 4 35.416076634735525 37.0 37.0 37.0 33.0 37.0 5 35.475322782174096 37.0 37.0 37.0 33.0 37.0 6 34.76228654727197 37.0 37.0 37.0 27.0 37.0 7 34.45564348188255 37.0 37.0 37.0 27.0 37.0 8 34.422636401499375 37.0 37.0 37.0 27.0 37.0 9 34.321324448146605 37.0 37.0 37.0 27.0 37.0 10 34.296230737192836 37.0 37.0 37.0 27.0 37.0 11 34.285193669304455 37.0 37.0 37.0 27.0 37.0 12 34.33433985839233 37.0 37.0 37.0 27.0 37.0 13 34.24718867138692 37.0 37.0 37.0 27.0 37.0 14 35.50780924614744 37.0 37.0 40.0 27.0 40.0 15 35.55320699708455 37.0 37.0 40.0 27.0 40.0 16 35.594960433152856 37.0 37.0 40.0 27.0 40.0 17 35.590483132028325 37.0 37.0 40.0 27.0 40.0 18 35.57361516034985 37.0 37.0 40.0 27.0 40.0 19 35.57434402332362 37.0 37.0 40.0 27.0 40.0 20 35.48302790503957 37.0 37.0 40.0 27.0 40.0 21 35.48927530195752 37.0 33.0 40.0 27.0 40.0 22 35.42638483965015 37.0 33.0 40.0 27.0 40.0 23 35.43794252394836 37.0 33.0 40.0 27.0 40.0 24 35.3051853394419 37.0 37.0 40.0 27.0 40.0 25 33.95439400249896 37.0 33.0 40.0 15.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3984488909756223 1101 2 0.1810875023910654 1101 3 0.04221559737292324 1101 4 -1.2922505415739067 1101 5 -0.45448762690805466 1101 6 1.4111354367516071 1101 7 1.3129133606385395 1101 8 0.9766998341114288 1101 9 0.5870264757521682 1101 10 -0.32192813072504833 1101 11 -1.219728952838956 1101 12 1.6471634741704122 1101 13 -2.9523218849729282 1101 14 -2.4393233103245464 1101 15 -1.4906653175470979 1101 16 0.49571730547626913 1101 17 0.8628498228476573 1101 18 -0.7769296624535542 1101 19 -0.1309736190129982 1101 20 -0.8676130110080535 1101 21 -0.22684166223193358 1101 22 -0.38896310813153434 1101 23 1.4016737448299494 1101 24 0.23566849212772212 1101 25 0.08423030237199214 1102 1 1.4857616353401646 1102 2 1.202140133970012 1102 3 1.3264261236887123 1102 4 0.893714370706796 1102 5 0.8753369344954578 1102 6 0.5058722788568701 1102 7 1.1515098518666136 1102 8 1.4468752727079206 1102 9 1.2361492827697163 1102 10 2.5131595885732025 1102 11 2.769744731371574 1102 12 2.32084768469673 1102 13 0.8757482904656655 1102 14 2.988746865114045 1102 15 1.7128434543827282 1102 16 1.9483488844236376 1102 17 2.887411226356427 1102 18 1.6651756007043446 1102 19 3.0514825213378813 1102 20 2.220106287237556 1102 21 3.1661407939084185 1102 22 2.667177242745659 1102 23 2.7244807623738083 1102 24 1.5900544570399973 1102 25 0.09124784623163862 1103 1 1.801551109024377 1103 2 1.0310875023910668 1103 3 0.5797155973729247 1103 4 1.5910827917594261 1103 5 1.6121790397586153 1103 6 2.36113543675161 1103 7 2.7962466939718738 1103 8 2.710033167444763 1103 9 2.670359809085504 1103 10 2.8947385359416202 1103 11 2.9802710471610467 1103 12 2.597163474170415 1103 13 2.9810114483604053 1103 14 4.344010023008785 1103 15 3.2260013491195707 1103 16 4.145717305476268 1103 17 3.9795164895143245 1103 18 4.010570337546447 1103 19 3.985693047653669 1103 20 4.236553655658611 1103 21 3.6398250044347336 1103 22 3.3152035585351314 1103 23 3.372507078163281 1103 24 3.731501825461052 1103 25 5.055063635705324 1104 1 1.095668756083203 1104 2 0.7222639729793023 1104 3 1.2892744209023377 1104 4 1.3704945564653102 1104 5 1.3768849221115573 1104 6 0.846429554398668 1104 7 0.6933055175012868 1104 8 0.8276802262682921 1104 9 1.979183338497272 1104 10 2.085915006529852 1104 11 1.2155651648081047 1104 12 2.611869356523357 1104 13 1.9221879189486373 1104 14 0.741068846538198 1104 15 1.2112954667666287 1104 16 2.6015996584174417 1104 17 2.6706929601025564 1104 18 2.3524821022523312 1104 19 2.735693047653669 1104 20 3.0343477733056687 1104 21 -0.4189985249770345 1104 22 2.7600564997116024 1104 23 1.582065901692694 1104 24 1.4042959431081101 1104 25 1.2057989298229685 1105 1 0.3897864031420255 1105 2 0.36932279650871536 1105 3 1.2892744209023377 1105 4 0.48814161528883915 1105 5 1.0239437456409703 1105 6 1.905253083810436 1105 7 1.5168349292659897 1105 8 1.7688566968565311 1105 9 1.5085951032031488 1105 10 1.3212091241769173 1105 11 0.4508592824551627 1105 12 0.847163474170415 1105 13 -0.19545913987489172 1105 14 -0.7295193887559179 1105 15 1.6230601726489837 1105 16 -0.16310622393550034 1105 17 -0.38813056930920453 1105 18 -1.5298708389241398 1105 19 -0.49960106999338905 1105 20 -0.4950639914002153 1105 21 1.6398250044347336 1105 22 -0.8281787944060426 1105 23 0.17030119581033887 1105 24 -0.6545275863036508 1105 25 -1.794201070177028 1106 1 -1.8984488909756223 1106 2 -1.1189124976089317 1106 3 -1.257784402627074 1106 4 1.041082791759429 1106 5 -1.1878209602413818 1106 6 -0.18886456324838719 1106 7 1.4462466939718723 1106 8 -1.1899668325552355 1106 9 1.0203598090855053 1106 10 1.7447385359416216 1106 11 -0.5197289528389533 1106 12 1.4471634741704165 1106 13 0.7810114483604025 1106 14 -0.30598997699121355 1106 15 -2.723998650880432 1106 16 1.5957173054762706 1106 17 -1.0704835104856727 1106 18 0.023070337546450048 1106 19 1.735693047653669 1106 20 2.099053655658608 1106 21 -0.760174995565265 1106 22 2.477703558535133 1106 23 -0.26499292183672196 1106 24 -3.1309981745389486 1106 25 0.8175636357053264 1107 1 1.1348844423577127 1107 2 0.4477541690577347 1107 3 -0.35778440262707534 1107 4 -0.3811394304627953 1107 5 0.05662348420305818 1107 6 0.38891321452938854 1107 7 1.0462466939718738 1107 8 0.7100331674447631 1107 9 -2.4685290798033854 1107 10 -1.1885947973917155 1107 11 0.20249326938326817 1107 12 -0.04172541471847069 1107 13 -1.0189885516395947 1107 14 -1.1282121992134364 1107 15 0.4760013491195707 1107 16 3.062383972142932 1107 17 2.11840537840321 1107 18 2.323070337546447 1107 19 1.3468041587647832 1107 20 1.6879425445474965 1107 21 -0.4712861066763807 1107 22 1.0443702252017957 1107 23 2.101673744829945 1107 24 0.113446269905495 1107 25 2.5620080801497664 1108 1 -1.0317822243089552 1108 2 0.7810875023910668 1108 3 0.3088822640395925 1108 4 0.0077494584260904276 1108 5 0.6121790397586153 1108 6 -1.38886456324839 1108 7 -0.787086639361462 1108 8 -0.9010779436663512 1108 9 -1.3018624131367176 1108 10 -0.7997059085028226 1108 11 -1.5752845083945104 1108 12 -3.486169859162917 1108 13 -3.1856552183062625 1108 14 -2.2948788658801007 1108 15 -3.13510976199154 1108 16 -1.493171583412618 1108 17 -2.1038168438190112 1108 18 -0.3991518846757742 1108 19 -1.8198625079018882 1108 20 -1.4787241221191678 1108 21 -1.2490638844541522 1108 22 -0.6778519970204258 1108 23 -1.7316595885033834 1108 24 -0.8309981745389479 1108 25 -0.660214142072455 1109 1 1.44860993255379 1109 2 1.7810875023910668 1109 3 1.6422155973729247 1109 4 1.8410827917594261 1109 5 1.6121790397586153 1109 6 0.846429554398668 1109 7 1.1638937527954027 1109 8 2.004150814503589 1109 9 2.21447745614433 1109 10 2.4388561830004463 1109 11 2.3920357530434018 1109 12 2.25892818005277 1109 13 2.1574820365957024 1109 14 3.446951199479372 1109 15 0.8583542902960417 1109 16 0.5427761290056807 1109 17 1.7295164895143245 1109 18 1.4701291610758602 1109 19 2.323928341771314 1109 20 2.4461124791880238 1109 21 3.3457073573759075 1109 22 2.9953506173586604 1109 23 0.052654136986809874 1109 24 1.992531237225755 1109 25 1.9116812827641496 1110 1 -0.09318573308088673 1110 2 -0.11364933971419688 1110 3 -0.8841001921007603 1110 4 -0.6852329977142588 1110 5 0.5595474608112454 1110 6 -0.12570666851154755 1110 7 -0.11164804287022889 1110 8 0.3942436937605507 1110 9 0.3940440196118189 1110 10 0.09210695699425031 1110 11 -1.7565710581021108 1110 12 0.6366371583809425 1110 13 -2.0189885516395947 1110 14 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0.013364364299796705 2209 17 -0.27048351048567554 2209 18 -1.0592826036300238 2209 19 -1.205483422934563 2209 20 0.6814065968350818 2209 21 -0.7131161720358534 2209 22 -0.5928846767589846 2209 23 0.052654136986809874 2209 24 -3.7721746451271834 2209 25 -2.7353775407652634 2210 1 1.3729796804529464 2210 2 1.3525160738196362 2210 3 0.85650131165864 2210 4 0.48393993461657203 2210 5 0.04075046833004592 2210 6 -0.6031502775341053 2210 7 1.760532408257589 2210 8 1.710033167444763 2210 9 -0.29392590520021145 2210 10 -0.4981186069155257 2210 11 -0.37687180998180736 2210 12 1.847163474170415 2210 13 1.26672573407469 2210 14 -0.405989976991215 2210 15 0.0474299205481401 2210 16 0.1814315911905524 2210 17 1.0866593466571786 2210 18 1.1802131946893013 2210 19 0.23569304765366894 2210 20 0.15619651280146485 2210 21 1.4255392901490183 2210 22 0.9491321299637008 2210 23 0.006435649591850279 2210 24 -0.045283888824663165 2210 25 0.11756363570532358 2211 1 0.6682177756910441 2211 2 -0.6189124976089317 2211 3 -2.157784402627076 2211 4 -0.15891720824057387 2211 5 -1.7878209602413833 2211 6 -0.9888645632483914 2211 7 -0.22041997269479197 2211 8 -1.156633499221904 2211 9 0.4536931424188353 2211 10 0.07807186927495025 2211 11 0.8469377138277139 2211 12 1.2471634741704136 2211 13 1.4476781150270739 2211 14 -0.30598997699121355 2211 15 -0.8906653175470964 2211 16 1.1623839721429334 2211 17 2.3961831561809888 2211 18 1.4897370042131115 2211 19 0.8023597143203389 2211 20 -0.3342796776747221 2211 21 -0.36017499556526644 2211 22 -0.6556297747982001 2211 23 0.735007078163278 2211 24 -0.8976648412056178 2211 25 -2.1157696976280107 2212 1 -1.135948890975623 2212 2 0.28108750239106683 2212 3 -0.23278440262707534 2212 4 0.5910827917594261 2212 5 -1.8253209602413847 2212 6 -1.82636456324839 2212 7 -0.45375330602812625 2212 8 0.21003316744476308 2212 9 -0.26714019091449615 2212 10 -1.2927614640583798 2212 11 -1.0822289528389533 2212 12 0.47216347417041504 2212 13 -0.8939885516395947 2212 14 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-1.967679660206393 2214 2 -0.9881432668397032 2214 3 -1.973169018011692 2214 4 -1.0819941313174937 2214 5 -0.541667114087538 2214 6 -0.0042491786330032255 2214 7 -0.18452253679735975 2214 8 -2.289966832555237 2214 9 -0.31040942168372965 2214 10 -1.3167999255968397 2214 11 -3.250498183608183 2214 12 -2.691298064291125 2214 13 -4.480527013178055 2214 14 -4.675220746221985 2214 15 -1.7163063431881227 2214 16 -2.7196673099083455 2214 17 -4.193560433562599 2214 18 -2.9461604316843193 2214 19 -5.418153106192484 2214 20 -2.508638652033696 2214 21 -2.05248268787296 2214 22 -2.5838349030033285 2214 23 -2.7573006141444125 2214 24 0.24592490238413234 2214 25 -3.9208979027562165 2215 1 0.0015511090243762737 2215 2 0.08108750239106755 2215 3 0.5422155973729232 2215 4 -1.3589172082405767 2215 5 -2.3878209602413847 2215 6 -1.9888645632483914 2215 7 1.4462466939718723 2215 8 0.9100331674447659 2215 9 1.5203598090855053 2215 10 -2.6552614640583805 2215 11 0.18027104716104958 2215 12 -0.5528365258295835 2215 13 0.5810114483604067 2215 14 -1.3059899769912136 2215 15 0.17600134911957355 2215 16 2.2957173054762663 2215 17 0.4295164895143273 2215 18 -0.1769296624535528 2215 19 1.1356930476536675 2215 20 0.9990536556586136 2215 21 -1.9601749955652679 2215 22 0.6777035585351285 2215 23 0.0350070781632823 2215 24 0.26900182546105356 2215 25 -0.782436364294675 2216 1 -2.4841631766899077 2216 2 0.06680178810535153 2216 3 -1.7863558311985024 2216 4 -2.4446314939548586 2216 5 -2.1021066745271 2216 6 -3.2460074203912477 2216 7 -3.810896163170984 2216 8 -1.0042525468409522 2216 9 -1.9367830480573502 2216 10 -1.1409757497726645 2216 11 -3.0197289528389533 2216 12 -4.581407954401012 2216 13 -2.161845694496737 2216 14 -0.7631328341340691 2216 15 -1.45257007945186 2216 16 -3.389996980238017 2216 17 -2.2704835104856755 2216 18 -1.1769296624535528 2216 19 -4.407164095203473 2216 20 -6.058089201484247 2216 21 -3.2173178527081205 2216 22 -6.550867870036296 2216 23 -4.493564350408146 2216 24 0.8832875397467674 2216 25 -2.5252935071518188 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 6.0 12 13.0 13 21.0 14 35.0 15 49.0 16 61.0 17 47.0 18 47.0 19 64.0 20 74.0 21 79.0 22 79.0 23 78.0 24 91.0 25 96.0 26 95.0 27 93.0 28 101.0 29 129.0 30 153.0 31 191.0 32 295.0 33 397.0 34 570.0 35 860.0 36 1594.0 37 4284.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.77821823492593 19.653661589818487 12.904235343208848 26.663884832046737 2 14.369012911286964 20.324864639733445 40.04581424406498 25.260308204914615 3 17.77384423157018 25.447730112453144 29.9875052061641 26.790920449812578 4 12.119950020824657 16.795085381091212 38.24448146605581 32.84048313202832 5 11.786755518533944 38.10912119950021 35.537276134943774 14.566847147022074 6 30.48729695960017 38.73386089129529 16.78467305289463 13.994169096209912 7 26.228654727197004 32.715535193669304 22.844648063306956 18.21116201582674 8 24.906289046230736 36.8179925031237 20.71012078300708 17.56559766763848 9 25.208246563931695 15.972511453561017 21.845064556434817 36.974177426072465 10 15.316534777176177 29.498125780924617 32.91336942940441 22.271970012494794 11 31.559766763848394 25.499791753436067 22.553102873802583 20.387338608912952 12 21.220324864639732 27.71761765930862 31.50770512286547 19.55435235318617 13 27.75926697209496 23.094543940024987 25.65597667638484 23.49021241149521 14 20.147855060391503 23.386089129529363 29.039983340274883 27.426072469804247 15 24.937526030820493 28.904623073719282 24.073302790503956 22.084548104956266 16 21.241149521032902 30.955851728446483 26.322365680966264 21.480633069554354 17 20.21032902957101 30.3935860058309 27.71761765930862 21.678467305289463 18 21.012078300708037 28.63390254060808 29.40441482715535 20.94960433152853 19 20.6268221574344 27.95710120783007 30.341524364847984 21.074552269887548 20 22.85506039150354 28.082049146189085 28.758850478967098 20.304039983340274 21 22.1990837151187 28.331945022907124 28.49854227405248 20.9704289879217 22 21.1057892544773 27.95710120783007 29.44606413994169 21.491045397750938 23 21.720116618075803 28.269471053727614 29.154518950437318 20.855893377759266 24 21.438983756768014 28.196584756351523 28.76926280716368 21.595168679716785 25 21.501457725947525 27.790503956684713 27.728029987505202 22.98000832986256 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6.0 1 6.0 2 5.0 3 4.0 4 4.0 5 4.0 6 10.5 7 17.0 8 17.0 9 17.0 10 26.0 11 35.0 12 35.0 13 35.0 14 50.0 15 65.0 16 65.0 17 65.0 18 119.5 19 174.0 20 174.0 21 174.0 22 257.5 23 341.0 24 341.0 25 341.0 26 423.0 27 505.0 28 505.0 29 505.0 30 636.5 31 768.0 32 768.0 33 768.0 34 863.0 35 958.0 36 958.0 37 958.0 38 1018.0 39 1078.0 40 1078.0 41 1078.0 42 1071.0 43 1064.0 44 1064.0 45 1064.0 46 1173.0 47 1282.0 48 1282.0 49 1282.0 50 1175.5 51 1069.0 52 1069.0 53 1069.0 54 955.0 55 841.0 56 841.0 57 841.0 58 712.0 59 583.0 60 583.0 61 583.0 62 508.0 63 433.0 64 433.0 65 433.0 66 322.5 67 212.0 68 212.0 69 212.0 70 166.0 71 120.0 72 120.0 73 120.0 74 76.0 75 32.0 76 32.0 77 32.0 78 22.5 79 13.0 80 13.0 81 13.0 82 8.0 83 3.0 84 3.0 85 3.0 86 1.5 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1874219075385256 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 9604.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.21116201582674 #Duplication Level Percentage of deduplicated Percentage of total 1 98.8047363717605 92.09704289879217 2 0.49151027703306527 0.9162848812994586 3 0.17873100983020554 0.49979175343606835 4 0.11170688114387846 0.41649312786339027 5 0.11170688114387846 0.5206164098292377 6 0.03351206434316354 0.1874219075385256 7 0.06702412868632708 0.43731778425655976 8 0.011170688114387846 0.08329862557267806 9 0.011170688114387846 0.0937109537692628 >10 0.15638963360142985 3.4256559766763846 >50 0.022341376228775692 1.322365680966264 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 64 0.6663890045814245 No Hit CCCATGTACTCTGCGTTGATACCAC 63 0.6559766763848397 No Hit GTATCAACGCAGAGTACTTTTTTTT 46 0.4789670970428988 No Hit GGTATCAACGCAGAGTACTTTTTTT 43 0.44773011245314454 No Hit CATGTACTCTGCGTTGATACCACTG 32 0.33319450229071224 No Hit GCGTTGATACCACTGCTTCCCATGT 26 0.27072053311120364 No Hit GAGTACATGGGAAGCAGTGGTATCA 26 0.27072053311120364 No Hit TATCAACGCAGAGTACTTTTTTTTT 24 0.24989587671803418 No Hit TATCAACGCAGAGTACATGGGAAGC 23 0.2394835485214494 No Hit GGTATCAACGCAGAGTACATGGGAA 20 0.20824656393169513 No Hit GTATCAACGCAGAGTACATGGGAAG 17 0.17700957934194084 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 17 0.17700957934194084 No Hit ACGCAGAGTACATGGGAAGCAGTGG 15 0.15618492294877134 No Hit ACTCTGCGTTGATACCACTGCTTCC 14 0.1457725947521866 No Hit GCTTCCCATGTACTCTGCGTTGATA 14 0.1457725947521866 No Hit GCAGTGGTATCAACGCAGAGTACAT 12 0.12494793835901709 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE