FastQCFastQC Report
Wed 25 May 2016
SRR1295045_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295045_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4025007
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC343720.8539612477692585No Hit
CCCATGTACTCTGCGTTGATACCAC314410.781141498635903No Hit
GAGTACATGGGAAGCAGTGGTATCA171350.42571354534290246No Hit
CATGTACTCTGCGTTGATACCACTG130850.3250926023234245No Hit
GCGTTGATACCACTGCTTCCCATGT103850.25801197364377254No Hit
ACGCAGAGTACATGGGAAGCAGTGG88230.21920458771872942No Hit
GCTTCCCATGTACTCTGCGTTGATA87840.21823564530446782No Hit
GTATCAACGCAGAGTACATGGGAAG87440.21724185821291742No Hit
GGTATCAACGCAGAGTACATGGGAA75020.18638476902027748No Hit
TATCAACGCAGAGTACATGGGAAGC74880.18603694353823483No Hit
ACTCTGCGTTGATACCACTGCTTCC74790.185813341442636No Hit
ATTCCATTCCATTCCATTCCATTCC54600.13565193799662956No Hit
GTATCAACGCAGAGTACTTTTTTTT48380.12019854872302085No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN47630.11833519792636385No Hit
GTGGTATCAACGCAGAGTACATGGG46950.11664575987072817No Hit
CAGTGGTATCAACGCAGAGTACATG40950.10173895349747217No Hit
GGTATCAACGCAGAGTACTTTTTTT40440.10047187495574542No Hit
GAATGGAATGGAATGGAATGGAATG40440.10047187495574542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAC400.005263578614.2571123
ACCCGTC600.00588983211.08044216
GTCTAGG3950.010.1131121
GTACGGG950.00181547259.0050534
GTCTTAT5100.08.9516621
GTATAGA6650.08.8675311
TATTCCG1401.0799415E-58.8253925
GACTGTC2950.08.6938387
GTCTCGG1107.065669E-48.6464921
TTAGACA5400.08.6252114
GACAGCG1000.00290973658.5489427
GTGTTAG6900.08.5462431
TCTATAC5600.08.4863773
GTCCTAG4300.08.4051941
GTCTAAG3850.08.3994491
TGGACAG5550.08.3911555
TAGACAG7150.08.3743915
CTTAGAC4200.08.3732253
GTCTTAA4550.08.36144351
GTTCTAG4100.08.3512471
Produced by FastQC (version 0.11.5)