FastQCFastQC Report
Wed 25 May 2016
SRR1295045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295045_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4025007
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC346200.860122727736871No Hit
GTACATGGGAAGCAGTGGTATCAAC338370.8406693454197719No Hit
GAGTACATGGGAAGCAGTGGTATCA184730.4589557235552634No Hit
CATGTACTCTGCGTTGATACCACTG145220.36079440358737264No Hit
GTATCAACGCAGAGTACTTTTTTTT123800.30757710483484874No Hit
GGTATCAACGCAGAGTACTTTTTTT113590.28221068932302473No Hit
GCGTTGATACCACTGCTTCCCATGT111850.27788771547478053No Hit
ACGCAGAGTACATGGGAAGCAGTGG108670.26998710809695486No Hit
GCTTCCCATGTACTCTGCGTTGATA96750.24037225276875296No Hit
GTATCAACGCAGAGTACATGGGAAG91840.2281735162199718No Hit
TATCAACGCAGAGTACTTTTTTTTT89100.22136607464285155No Hit
TATCAACGCAGAGTACATGGGAAGC83460.20735367665199092No Hit
ACTCTGCGTTGATACCACTGCTTCC81550.20260834328983773No Hit
GGTATCAACGCAGAGTACATGGGAA80430.19982573943349663No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55560.13803702701635054No Hit
ATTCCATTCCATTCCATTCCATTCC51860.12884449641950932No Hit
GTGGTATCAACGCAGAGTACATGGG49810.12375133757531354No Hit
CAGTGGTATCAACGCAGAGTACATG49480.12293146322478446No Hit
ACATGGGAAGCAGTGGTATCAACGC46070.11445942826931729No Hit
GAATGGAATGGAATGGAATGGAATG44300.11006192038920677No Hit
CATGGGAAGCAGTGGTATCAACGCA41750.10372652768057297No Hit
GCAGTGGTATCAACGCAGAGTACAT40700.10111783656525318No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG550.00304042512.1064961
CTAGGAC4400.011.87420753
AACGCCG658.0327777E-411.6915286
GGGACCG752.0766638E-411.399245
ACGCCGA700.001492970910.8565547
ACCGCCT700.001492970910.8565548
GTCCTAG3800.010.51353551
CAATCCG1106.057495E-610.36281710
CGCCGAG750.002650329110.1327838
CAGGACG856.601842E-410.0581534
GACCGGG1002.7587867E-49.4994847
TAGGACT5150.09.407144
CTAATAC5600.09.3297353
CCAGGAC5500.09.1539353
TAGGCGT1252.75858E-59.1193926
TACGTTG950.00182587768.9993995
TTCGAGT1701.6894592E-78.94035818
CTTGGAC3100.08.8865033
GTACTAG3600.08.719561
TATACTG10250.08.7116145
Produced by FastQC (version 0.11.5)