FastQCFastQC Report
Wed 25 May 2016
SRR1295044_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295044_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2932000
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC175820.5996589358799455No Hit
CCCATGTACTCTGCGTTGATACCAC156260.5329467939972715No Hit
GAGTACATGGGAAGCAGTGGTATCA83810.28584583901773536No Hit
CATGTACTCTGCGTTGATACCACTG64160.21882673942701228No Hit
GCTTCCCATGTACTCTGCGTTGATA53550.18263983628922237No Hit
GCGTTGATACCACTGCTTCCCATGT50880.17353342428376534No Hit
GTATCAACGCAGAGTACATGGGAAG44990.15344474761255117No Hit
ACGCAGAGTACATGGGAAGCAGTGG43640.14884038199181446No Hit
GTATCAACGCAGAGTACTTTTTTTT42220.14399727148703956No Hit
GGTATCAACGCAGAGTACATGGGAA40390.13775579809004093No Hit
TATCAACGCAGAGTACATGGGAAGC37520.12796725784447474No Hit
GGTATCAACGCAGAGTACTTTTTTT36420.12421555252387448No Hit
ACTCTGCGTTGATACCACTGCTTCC35620.12148703956343793No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN35000.11937244201909959No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG550.003046376212.1031541
GAACCGT550.003071461812.0899096
GGACCGT550.003071461812.0899096
TCCGTAC803.774512E-410.686988
CTAGACT3150.010.5615444
CGCAAAA1201.5175574E-610.2955682
CTGAGCG750.002650208510.13266859
ACTGTCC3300.010.0752688
GGGTATA2800.09.849251
CCTACAC3400.09.5037643
GACCGTG1002.75805E-49.4995387
CTGTGCG1304.2450392E-69.4993769
GCACGAT1252.7655298E-59.11721816
GCTATAA3150.09.0567811
TGTGCGG1054.4997202E-49.0465610
CTAAGAC3050.09.03636553
GTCCTAC1901.0406438E-89.0091141
GTACAGG4550.08.9871141
TAAGACA5550.08.9059514
GCTACAC2252.6011548E-108.8701793
Produced by FastQC (version 0.11.5)