FastQCFastQC Report
Wed 25 May 2016
SRR1295044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295044_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2932000
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC174150.5939631650750341No Hit
CCCATGTACTCTGCGTTGATACCAC170080.5800818553888131No Hit
GTATCAACGCAGAGTACTTTTTTTT114110.38918826739427015No Hit
GGTATCAACGCAGAGTACTTTTTTT106100.36186903137789905No Hit
GAGTACATGGGAAGCAGTGGTATCA93840.32005457025920875No Hit
TATCAACGCAGAGTACTTTTTTTTT79480.27107776261937244No Hit
CATGTACTCTGCGTTGATACCACTG70280.239699863574352No Hit
GCTTCCCATGTACTCTGCGTTGATA57730.19689631650750342No Hit
ACGCAGAGTACATGGGAAGCAGTGG56330.19212141882673944No Hit
GCGTTGATACCACTGCTTCCCATGT53550.18263983628922237No Hit
GTATCAACGCAGAGTACATGGGAAG48960.1669849931787176No Hit
ACGCAGAGTACTTTTTTTTTTTTTT46330.15801500682128242No Hit
GGTATCAACGCAGAGTACATGGGAA44690.15242155525238746No Hit
TATCAACGCAGAGTACATGGGAAGC44030.15017053206002728No Hit
ACTCTGCGTTGATACCACTGCTTCC39830.13584583901773534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAA550.00307148112.089919
CCAGGAC4500.011.1883373
AGGGACG600.005879924611.0827875
CCGTGCA600.005881417511.0824089
CGTAGAC803.774546E-410.6869723
GTAGGAC1909.094947E-1210.4994823
AGCACCG750.002649854210.1328335
GAACCGT750.00265022910.1326616
AGAACCG856.5996544E-410.0583275
TAATACT4850.09.5974654
CTAGGAC3200.09.4995313
TATACTG6900.09.0865085
TCTATAC4000.09.0245553
TAGACAG4550.08.9775795
AGCGTCA850.00744105368.9407353
GTCTAGA2800.08.8330441
GTGTAGG3500.08.6971511
TGGACAG4700.08.4889435
GTGCTAG4150.08.4810321
TTACACT5500.08.4632194
Produced by FastQC (version 0.11.5)