FastQCFastQC Report
Wed 25 May 2016
SRR1295043_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295043_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6798418
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC382020.5619248478101817No Hit
CCCATGTACTCTGCGTTGATACCAC362840.5337124019146808No Hit
GAGTACATGGGAAGCAGTGGTATCA172500.2537355014063566No Hit
ATTCCATTCCATTCCATTCCATTCC170100.25020526834331164No Hit
CATGTACTCTGCGTTGATACCACTG144540.2126082862218828No Hit
GAATGGAATGGAATGGAATGGAATG127390.18738182912554066No Hit
GCGTTGATACCACTGCTTCCCATGT113100.1663622330959938No Hit
ACGCAGAGTACATGGGAAGCAGTGG94540.13906176407511278No Hit
GTATCAACGCAGAGTACATGGGAAG92420.13594339153608972No Hit
GTATCAACGCAGAGTACTTTTTTTT89000.13091280942125064No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN80580.11852757509173459No Hit
TATCAACGCAGAGTACATGGGAAGC79620.1171154818665166No Hit
ACTCTGCGTTGATACCACTGCTTCC78390.11530623742170606No Hit
GGTATCAACGCAGAGTACATGGGAA76650.11274681845099845No Hit
GCTTCCCATGTACTCTGCGTTGATA69450.10215611926186358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG1951.8189894E-1210.7239395
CTGTCGC3950.010.3408379
TCGGAAC2550.010.05509713
TATTCCG6300.09.8070655
GAACGAT2850.09.66351216
CGAATTA2252.7284841E-119.28572115
TAGACAG9550.09.1569475
CACGACT1054.4690623E-49.0531434
GGAACGA6400.09.051615
GTATTAG8050.08.9796541
TAGGACT7000.08.9626114
GTCGCCC3500.08.95435211
GCACCGA1606.7641486E-78.9051176
ACTCGGA3750.08.86390411
CGTACCA5000.08.54878510
TCTGTCG4450.08.5385538
TGTCGCC4350.08.51603110
CCTACAC6600.08.4967313
CTAGGAC6400.08.4652293
GTCTTAG6900.08.4085791
Produced by FastQC (version 0.11.5)