##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295043_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6798418
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40198278481847	33.0	33.0	33.0	27.0	33.0
2	31.423638852450672	33.0	33.0	33.0	27.0	33.0
3	31.422822486054844	33.0	33.0	33.0	27.0	33.0
4	35.133425894083004	37.0	37.0	37.0	33.0	37.0
5	35.27759884726123	37.0	37.0	37.0	33.0	37.0
6	35.30649645255705	37.0	37.0	37.0	33.0	37.0
7	35.237618516543115	37.0	37.0	37.0	33.0	37.0
8	35.08649218097504	37.0	37.0	37.0	33.0	37.0
9	35.09344718138838	37.0	37.0	37.0	33.0	37.0
10	35.00217991891643	37.0	37.0	37.0	33.0	37.0
11	35.15485176110089	37.0	37.0	37.0	33.0	37.0
12	35.06955632913422	37.0	37.0	37.0	33.0	37.0
13	35.01103903878814	37.0	37.0	37.0	33.0	37.0
14	36.48889271003931	40.0	37.0	40.0	33.0	40.0
15	36.64322229083295	40.0	37.0	40.0	33.0	40.0
16	36.72278212372349	40.0	37.0	40.0	33.0	40.0
17	36.64916440854328	40.0	37.0	40.0	33.0	40.0
18	36.595437056091576	40.0	37.0	40.0	33.0	40.0
19	36.70102441479768	40.0	37.0	40.0	33.0	40.0
20	36.69310595494422	40.0	37.0	40.0	33.0	40.0
21	36.701914327715656	40.0	37.0	40.0	33.0	40.0
22	36.6114154793071	40.0	37.0	40.0	33.0	40.0
23	36.52226459155645	40.0	37.0	40.0	33.0	40.0
24	36.56733595963061	40.0	37.0	40.0	33.0	40.0
25	35.535703747548325	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.45400682527481706
1101	2	0.37154701768233167
1101	3	0.422681774721255
1101	4	0.5413004224495594
1101	5	0.4056960680513839
1101	6	0.34601628931138606
1101	7	0.3961877451632674
1101	8	0.5166006375596339
1101	9	0.5709251402046007
1101	10	0.6793592491775016
1101	11	0.5772644451793454
1101	12	0.6079953661663637
1101	13	0.5317621443393037
1101	14	0.9956850278934297
1101	15	0.8373988824973466
1101	16	0.788215001491217
1101	17	0.7701466110192285
1101	18	0.8505830623368027
1101	19	0.7007196993968563
1101	20	0.8016346291963288
1101	21	0.7758607620468538
1101	22	0.8420662010454407
1101	23	0.9318120808347317
1101	24	0.8853705620781085
1101	25	1.035217627878552
1102	1	0.3663959601559412
1102	2	0.3798374605071153
1102	3	0.3705307132364055
1102	4	0.42466467629632376
1102	5	0.3020997462633943
1102	6	0.266494306994403
1102	7	0.27679445116859824
1102	8	0.3841987217057721
1102	9	0.40608042282146783
1102	10	0.612324075661455
1102	11	0.4646386891677494
1102	12	0.45956038413233813
1102	13	0.41726799927035074
1102	14	0.875665936541516
1102	15	0.7230950204264204
1102	16	0.7575842659134224
1102	17	0.6749302350426021
1102	18	0.8205615445123016
1102	19	0.7222561914513861
1102	20	0.7542674656497468
1102	21	0.7135746320897383
1102	22	0.744657771960334
1102	23	0.856513297817969
1102	24	0.816996554535109
1102	25	0.8951204811157254
1103	1	0.4206367688946706
1103	2	0.4239529898851977
1103	3	0.40186758565506153
1103	4	0.45344440210198655
1103	5	0.3246509199954417
1103	6	0.25499228475176494
1103	7	0.29835207489735893
1103	8	0.4105188681445142
1103	9	0.39048511143076325
1103	10	0.5321130182109854
1103	11	0.3834119625572612
1103	12	0.487453865683662
1103	13	0.43234044821461026
1103	14	0.7591355982857593
1103	15	0.7543772782681444
1103	16	0.6167618801201158
1103	17	0.6533387391406293
1103	18	0.715370872876413
1103	19	0.6780100527551269
1103	20	0.6738435885049512
1103	21	0.7070894876747786
1103	22	0.697383171456849
1103	23	0.8138451205293151
1103	24	0.8185214502129128
1103	25	0.8889401963827552
1104	1	0.32361225167050023
1104	2	0.35205122868641325
1104	3	0.3774734434892224
1104	4	0.3818475503934806
1104	5	0.2480892800136658
1104	6	0.3148686772280769
1104	7	0.29277173830463
1104	8	0.42723420455746464
1104	9	0.37828073050164335
1104	10	0.5798798503172478
1104	11	0.39500770000812935
1104	12	0.45144450631462973
1104	13	0.4753280831158406
1104	14	0.7491729496242314
1104	15	0.6786374724413662
1104	16	0.532372649380676
1104	17	0.6756856699732836
1104	18	0.7359402664318111
1104	19	0.6538279751545844
1104	20	0.5962066701464508
1104	21	0.6015539387073048
1104	22	0.6779613546084065
1104	23	0.6804276943301204
1104	24	0.7399955557742075
1104	25	0.8224389199255526
1105	1	0.3005762625838848
1105	2	0.22533926837087748
1105	3	0.2471513345778078
1105	4	0.2998547731358485
1105	5	0.21866890962430574
1105	6	0.15811296398916852
1105	7	0.24220962327238027
1105	8	0.34927928497623384
1105	9	0.28125071553872516
1105	10	0.4383277652502713
1105	11	0.29359322328820525
1105	12	0.4062015817547078
1105	13	0.36021031226695044
1105	14	0.6995146041898863
1105	15	0.5815197859905794
1105	16	0.47620046620681933
1105	17	0.48026939407509417
1105	18	0.4939875467226429
1105	19	0.4519552837779415
1105	20	0.4907806411403186
1105	21	0.5436426024644732
1105	22	0.4267250604265911
1105	23	0.6443891717228638
1105	24	0.6420438464759783
1105	25	0.6657626158368188
1106	1	0.04703900501517566
1106	2	-0.1184390183391244
1106	3	-0.15892147131023293
1106	4	-0.03543285908539673
1106	5	-0.31794589726528955
1106	6	-0.3243210260220124
1106	7	-0.6166679963628425
1106	8	-0.18222047459464363
1106	9	0.12226048072496098
1106	10	-0.27678555965411533
1106	11	-0.3835604655224145
1106	12	-0.8009396484558877
1106	13	-0.18439585149524618
1106	14	-0.3376607046758764
1106	15	-0.15377397299597817
1106	16	-0.10822336383839826
1106	17	-0.189427109938606
1106	18	-0.41751691931794
1106	19	-0.573184095913227
1106	20	-0.14002759144316457
1106	21	-0.0037499947489791907
1106	22	0.15866428882210215
1106	23	-0.5389056288137937
1106	24	0.10028856956729015
1106	25	0.1889379706111356
1107	1	-1.1301950357361648
1107	2	-1.0480669363073467
1107	3	-0.8344293709860828
1107	4	-0.9008946124593677
1107	5	-0.9921988578794227
1107	6	-0.9437413481387651
1107	7	-1.070634428890358
1107	8	-0.9302707802337409
1107	9	-0.923245991371509
1107	10	-0.7782035210223697
1107	11	-0.8572936904565012
1107	12	-0.8668594773721878
1107	13	-0.7762626212379828
1107	14	-1.0362876676983461
1107	15	-0.9399786967513037
1107	16	-0.9570484740725291
1107	17	-1.0928931839581963
1107	18	-1.1868439043590087
1107	19	-1.092844747389485
1107	20	-1.1999652175157678
1107	21	-1.1416950143923117
1107	22	-1.0974715401135597
1107	23	-0.8876139517629014
1107	24	-0.7903082997591966
1107	25	-0.8178612794660935
1108	1	0.03810227348036932
1108	2	-0.12162986664225528
1108	3	-0.014528144690249434
1108	4	-0.16942070253918473
1108	5	-0.47394673773303
1108	6	-0.09875358628092812
1108	7	-0.04574080430226246
1108	8	0.1272851966728652
1108	9	-0.1829374980894869
1108	10	-0.4966088287523789
1108	11	-0.08553061592551359
1108	12	0.08408088607498598
1108	13	0.1066681610602771
1108	14	-0.2552908797355258
1108	15	0.08149052867491946
1108	16	-0.8121844873628277
1108	17	-0.7212356132139064
1108	18	-0.5910088135758542
1108	19	-0.02382855490877489
1108	20	-1.0060240394157773
1108	21	-0.22103116191726002
1108	22	-0.36148062808426573
1108	23	-0.0750434385945411
1108	24	-0.40236349237788005
1108	25	-1.1554308423663713
1109	1	0.17571416983538057
1109	2	0.15991394944175852
1109	3	0.22900133833480396
1109	4	0.20928269279770717
1109	5	0.16677657763364806
1109	6	0.1591491048521334
1109	7	0.18795063721106686
1109	8	0.2679690622812245
1109	9	0.2536887816380755
1109	10	0.35430125602073304
1109	11	0.24561669130316233
1109	12	0.3271959749696123
1109	13	0.2887844458247173
1109	14	0.5286507246187924
1109	15	0.4951101773079998
1109	16	0.3729728918253059
1109	17	0.41641993347001005
1109	18	0.4249505401602107
1109	19	0.4221105127417317
1109	20	0.4039895359297603
1109	21	0.3475762743303932
1109	22	0.46962128670196535
1109	23	0.49631499414417135
1109	24	0.4937528533393376
1109	25	0.4704024124446917
1110	1	0.05924707634672188
1110	2	0.04461969584783532
1110	3	0.10092842906965416
1110	4	-0.9015847934439947
1110	5	-0.23024258283379595
1110	6	-0.008361410867181007
1110	7	0.12233881833585514
1110	8	0.1825296093675064
1110	9	0.17581427694082663
1110	10	0.01601065636909027
1110	11	0.047289435089787446
1110	12	0.10050926101189361
1110	13	0.0724535878188064
1110	14	0.2292164055579633
1110	15	0.09673776633346876
1110	16	0.13254083846464937
1110	17	0.18142398476751964
1110	18	0.2936599372987132
1110	19	0.20070447134984448
1110	20	0.2658254879988533
1110	21	0.2384057683765448
1110	22	-0.40595002503425803
1110	23	0.019262988090133604
1110	24	0.26506388089320865
1110	25	0.3584461762124249
1111	1	-0.08860908835492864
1111	2	-0.2426163461097559
1111	3	-0.15056260103602526
1111	4	-0.14232975262184766
1111	5	-0.2236852970135672
1111	6	-0.13228474757985964
1111	7	-0.1265057279777011
1111	8	0.055325934725942716
1111	9	-0.08495502071740901
1111	10	-0.08038387623470555
1111	11	-0.184991992798345
1111	12	-0.08645107806015773
1111	13	-0.2001267996071192
1111	14	-0.2742575913744716
1111	15	-0.154953648974967
1111	16	-0.27314561881853905
1111	17	-0.09213148189555653
1111	18	-0.18623837914647368
1111	19	-0.19092354163617387
1111	20	-0.18140376592657503
1111	21	-0.28363200789773657
1111	22	-0.1924161098925623
1111	23	-0.09796655784268893
1111	24	-0.08343577592972906
1111	25	-0.14698782605157135
1112	1	-0.04263738503415482
1112	2	-0.3389393175112154
1112	3	-0.05385119001121197
1112	4	-0.0725491356308865
1112	5	-0.013431334402255857
1112	6	-0.04005697584607759
1112	7	0.03232390571112376
1112	8	0.11100564349586506
1112	9	-0.01651913663606308
1112	10	0.20098225537756775
1112	11	0.055225776501430346
1112	12	0.10560823201897307
1112	13	0.014230515976159097
1112	14	-0.17191298303841762
1112	15	-0.15964022781334108
1112	16	-0.07814540526977964
1112	17	-0.24299059912340937
1112	18	-0.12345044439953057
1112	19	-0.002104525021415782
1112	20	-0.7506116298298977
1112	21	-1.2554731410118833
1112	22	-1.6334789286626759
1112	23	-0.5181820804357926
1112	24	-0.3207314952423914
1112	25	-1.5075160675554216
1113	1	-0.22569798270933106
1113	2	-0.17258829502410222
1113	3	-0.2535581424378748
1113	4	-0.46019984535497827
1113	5	-0.2558218034381454
1113	6	-0.1562802952880702
1113	7	-0.17790928934780936
1113	8	-0.07634133812371857
1113	9	-0.19260392551463212
1113	10	-0.021348522510201917
1113	11	-0.14919033156093775
1113	12	-0.13758850128824207
1113	13	-0.19527413424882667
1113	14	-0.5847619735884209
1113	15	-0.47452301677243724
1113	16	-0.4775922863629489
1113	17	-0.4894864342754417
1113	18	-0.9349915212271398
1113	19	-1.055571591599552
1113	20	-1.232592870759035
1113	21	-1.029006049829384
1113	22	-0.42829591532455424
1113	23	-1.9756669679235515
1113	24	-1.0436198415926015
1113	25	-0.6002859071413553
1114	1	-0.234495290306441
1114	2	-0.41121652203770864
1114	3	-0.27901550323086255
1114	4	-0.4441889026800183
1114	5	-0.5883911908604986
1114	6	-0.41147538940234085
1114	7	-0.3545590638350049
1114	8	-0.12142928910022022
1114	9	-0.9911700873966822
1114	10	-0.32481855661213643
1114	11	-0.4358145541507241
1114	12	-0.3539818384455984
1114	13	-0.35633322430072667
1114	14	-0.6989316375979158
1114	15	-1.3270851801238592
1114	16	-1.1522177494696066
1114	17	-1.4993691941357383
1114	18	-1.6691455879223582
1114	19	-1.553743965495073
1114	20	-0.836075968102783
1114	21	-0.6511072133418807
1114	22	-0.42070560707134064
1114	23	-0.33057474438300005
1114	24	-0.953333565068327
1114	25	-0.5956963384837053
1115	1	-0.741677840252585
1115	2	-0.486632764722966
1115	3	-0.5618857963991175
1115	4	-0.5979879639936883
1115	5	-0.38704087742621596
1115	6	-0.4378332126554483
1115	7	-0.4197864589612905
1115	8	-0.17821516473384946
1115	9	-0.6105600491496261
1115	10	-0.3192289679223137
1115	11	-0.4770888253139205
1115	12	-0.5838362138972855
1115	13	-0.5076252853632894
1115	14	-0.4714430070047939
1115	15	-1.6098181300548333
1115	16	-0.9534822902290685
1115	17	-0.7292969130031253
1115	18	-2.281708694405175
1115	19	-1.2420337650736073
1115	20	-1.0366731118860812
1115	21	-0.9766123037319616
1115	22	-0.8003180837077934
1115	23	-0.4706168142574043
1115	24	-0.49679407773295736
1115	25	-0.610533570946977
1116	1	-1.4503512651550423
1116	2	-0.8776072512115682
1116	3	-1.718525806684358
1116	4	-1.0029097753373293
1116	5	-0.998149291504177
1116	6	-0.5582244754394452
1116	7	-1.205597214692638
1116	8	-3.0635859983773983
1116	9	-1.5177783347357021
1116	10	-0.7998158154556521
1116	11	-0.6383547321523011
1116	12	-0.836364591998155
1116	13	-1.7986333828576377
1116	14	-1.7575900448361992
1116	15	-1.5302496462179676
1116	16	-1.0991408829266192
1116	17	-1.8171122088697018
1116	18	-2.6443947677414954
1116	19	-1.5166249542126735
1116	20	-1.084163298328022
1116	21	-0.9751877757631249
1116	22	-0.6760661086569044
1116	23	-0.6947047470774734
1116	24	-0.7316733019588
1116	25	-0.6862630198837678
1201	1	0.2853705499043855
1201	2	0.3136453384082891
1201	3	0.33037616210103593
1201	4	0.3692544644592104
1201	5	0.31737269176549177
1201	6	0.130802348324508
1201	7	0.20418452856937108
1201	8	0.28072481773543245
1201	9	0.23552471951342113
1201	10	0.4107891311797047
1201	11	0.2798521949728112
1201	12	0.32752550557364657
1201	13	0.3919022420006826
1201	14	0.809111969970175
1201	15	0.652765513438446
1201	16	0.669650460819021
1201	17	0.6677944689719624
1201	18	0.5731564512279164
1201	19	0.6016012167810771
1201	20	0.6223900685109172
1201	21	0.624371238601789
1201	22	0.6817671405546051
1201	23	0.6897700918730294
1201	24	0.6462731010928806
1201	25	0.7762086128828614
1202	1	0.3376141962293815
1202	2	0.30111969859322585
1202	3	0.3443627645928622
1202	4	0.4238050451561719
1202	5	0.29185032054011373
1202	6	0.060343828886956885
1202	7	0.11102316748613106
1202	8	0.22981837539177263
1202	9	0.24665493746839218
1202	10	0.428379225018503
1202	11	0.2214677898930404
1202	12	0.1736671326345558
1202	13	0.25083789820752855
1202	14	0.6315113265043522
1202	15	0.4236179473913211
1202	16	0.37476067119135337
1202	17	0.4438892594203452
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2210	21	0.4883507875929425
2210	22	0.5407567489945819
2210	23	0.5941337318580082
2210	24	0.5885383912350832
2210	25	0.6368404069479894
2211	1	0.10161828855110855
2211	2	-0.05293129699752441
2211	3	0.06140371708009695
2211	4	0.026971781773653447
2211	5	0.11370442412885495
2211	6	0.06207333427111905
2211	7	0.0133305002329962
2211	8	0.12313547987405116
2211	9	0.06102642690598259
2211	10	0.23007605076242044
2211	11	0.03235508495747297
2211	12	0.11200703034113246
2211	13	-0.0237436314918682
2211	14	-0.026637836371349977
2211	15	0.041787748638881794
2211	16	-0.047277940963894594
2211	17	-0.0069052711280619405
2211	18	0.07180757334271703
2211	19	0.004571906786260627
2211	20	0.04148058228676632
2211	21	0.030959326411505117
2211	22	0.007716876641964632
2211	23	0.13007580764064386
2211	24	0.09757200339707595
2211	25	0.08741361292484129
2212	1	0.06858895229366269
2212	2	0.049841696237290734
2212	3	0.08217636328987155
2212	4	0.06572942567258622
2212	5	0.10193851443266766
2212	6	0.06188683370650949
2212	7	0.16229165266443601
2212	8	0.19961732735397675
2212	9	0.001030099031396503
2212	10	0.24709353998932215
2212	11	0.11710689779729933
2212	12	0.11652318508888726
2212	13	0.009000169361748078
2212	14	0.09335161066193365
2212	15	0.06309212260720187
2212	16	0.01035657852420968
2212	17	0.01876568144245283
2212	18	0.08189047930546423
2212	19	0.0731749055747315
2212	20	0.08664756361989134
2212	21	0.0693371083339187
2212	22	-0.5642628683309141
2212	23	0.030442889575880372
2212	24	0.04506240784507298
2212	25	0.06422884276104668
2213	1	-0.05407552396238913
2213	2	-0.7830352388587798
2213	3	-0.22598411892944625
2213	4	-0.04859627789851828
2213	5	-0.009714784083534767
2213	6	-0.031257330915451575
2213	7	-0.03875605467459309
2213	8	0.1395333138659609
2213	9	-0.206266512539365
2213	10	-1.4829139092143464
2213	11	-0.422641669581715
2213	12	-0.17425262715512702
2213	13	-0.11662607788121448
2213	14	-0.04210188517298974
2213	15	-0.06067328004223782
2213	16	-0.12426394662200124
2213	17	-0.14866699303789943
2213	18	-0.10511119631931365
2213	19	-0.08044426079976574
2213	20	-0.09282212670358803
2213	21	-0.058212041137942094
2213	22	-0.03467458227496678
2213	23	-0.9310605152373697
2213	24	-1.5192846731670357
2213	25	-1.4848727381997193
2214	1	-0.03374565933077989
2214	2	-0.22179200847014968
2214	3	-0.014867178502893097
2214	4	-0.05353540476639296
2214	5	-0.0752767317111207
2214	6	-0.09732558761632504
2214	7	-0.040535007122699085
2214	8	-0.04825368695289001
2214	9	-0.40295971554311905
2214	10	-0.07521137299436731
2214	11	-0.17101025953840576
2214	12	-0.1411890807373979
2214	13	-0.07614481473919454
2214	14	-0.07901840635210533
2214	15	-0.11459593983125416
2214	16	-0.21219853990941573
2214	17	-0.221902834432953
2214	18	-0.0768744172373772
2214	19	-0.8365114775192808
2214	20	-0.4204166280042827
2214	21	-0.32533787324818775
2214	22	-0.6176220472557716
2214	23	-1.8257236444430873
2214	24	-1.3619487793186025
2214	25	-1.6892582987871094
2215	1	-0.38590126888243503
2215	2	-0.2371411525949796
2215	3	-1.2007736092658767
2215	4	-0.8426526480106489
2215	5	-0.2523059046313847
2215	6	-0.25879462807252906
2215	7	-0.2726123343490272
2215	8	-0.13272898952121892
2215	9	-0.2786097582389644
2215	10	-1.4189838312541525
2215	11	-0.5471168206002304
2215	12	-0.5003168276275716
2215	13	-0.47963723101382527
2215	14	-0.779999870063989
2215	15	-1.0260058642570726
2215	16	-0.7360435042177826
2215	17	-0.5653862801710048
2215	18	-0.48464918594898876
2215	19	-0.99179055779733
2215	20	-1.3685702467655716
2215	21	-0.6995058317695495
2215	22	-0.4319322195119426
2215	23	-0.6629478773781017
2215	24	-1.7750435198087544
2215	25	-0.9014014444852023
2216	1	-0.5531972320389151
2216	2	-0.5240730149294954
2216	3	-1.4776126824460114
2216	4	-0.8272690661143542
2216	5	-0.6900735806809166
2216	6	-0.6382324427780688
2216	7	-0.4622520134278503
2216	8	-0.5016885970046232
2216	9	-0.4185909050065675
2216	10	-0.5483341397008559
2216	11	-0.571146249700945
2216	12	-0.48683530179792456
2216	13	-1.175707300006998
2216	14	-0.899882423234331
2216	15	-1.3078347053281405
2216	16	-1.0305866948376448
2216	17	-1.9814585182235973
2216	18	-1.0219564053602568
2216	19	-0.8472782149879947
2216	20	-0.8596673471060328
2216	21	-0.6403259240627079
2216	22	-0.6370253786581372
2216	23	-0.5705552722220091
2216	24	-0.6274048670629071
2216	25	-0.5657732234656976
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8910.0
3	887.0
4	1091.0
5	1016.0
6	685.0
7	510.0
8	711.0
9	1222.0
10	2350.0
11	4346.0
12	7404.0
13	11349.0
14	15646.0
15	20399.0
16	23957.0
17	25827.0
18	26943.0
19	28093.0
20	29141.0
21	31338.0
22	34015.0
23	37013.0
24	40225.0
25	43486.0
26	46637.0
27	52919.0
28	60862.0
29	72511.0
30	89530.0
31	115717.0
32	156782.0
33	224516.0
34	332403.0
35	538562.0
36	998441.0
37	3712974.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.57362168683375	20.841289103285618	12.171457441967393	23.413631767913245
2	15.02346048013194	21.495535059716982	40.0660733650423	23.414931095108777
3	18.022576287234234	26.862385991783423	30.03402596797733	25.08101175300502
4	11.824405005846602	16.641718611887676	38.460642613144856	33.07323376912086
5	13.080226763134092	38.10555226401868	34.99524160749027	13.818979365356956
6	31.910689767589655	38.257243700431594	17.00229091292821	12.829775619050537
7	25.618434029478742	33.19244184984033	24.068008731734952	17.121115388945974
8	24.04781117522244	37.30339228555095	21.57560063953432	17.07319589969229
9	24.118259685143023	16.00875036760831	21.988762794427537	37.88422715282113
10	14.6559511195552	29.77652535037654	33.858146871029085	21.70937665903917
11	32.791463958174646	25.984423380316894	22.37319566842511	18.85091699308335
12	20.2732272091649	27.797742941551817	32.474263805774406	19.454766043508876
13	26.407711544437586	24.136757292556695	26.824576486564737	22.630954676440986
14	19.906948621423663	22.586656749519364	28.385269380704514	29.12112524835246
15	23.727582253367387	29.702636414404488	23.615288099575835	22.954493232652293
16	22.10466257536692	31.303660724274135	25.629815932781113	20.96186076757783
17	20.05159601876712	30.190952140071857	29.259449886154478	20.49800195500655
18	20.392984367648566	30.1270327540036	29.456876902160133	20.023105976187697
19	20.81808287601614	28.2245136558412	29.729430159587388	21.22797330855527
20	22.629993893668473	28.076655905786513	28.076449561008854	21.21690063953616
21	22.58070363988051	29.467462388805338	27.33353299096765	20.618300980346497
22	20.699885065162658	28.765825648656673	29.224842786298726	21.30944649988194
23	20.370017711136317	29.549356526824944	28.934124477922396	21.146501284116344
24	20.951188949331446	28.1529491545413	28.78281957159495	22.113042324532305
25	22.101949990396204	27.682440033037643	27.220013275790166	22.995596700775984
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13412.0
1	13412.0
2	10320.5
3	7229.0
4	7229.0
5	7229.0
6	10316.5
7	13404.0
8	13404.0
9	13404.0
10	19330.0
11	25256.0
12	25256.0
13	25256.0
14	41410.0
15	57564.0
16	57564.0
17	57564.0
18	90881.5
19	124199.0
20	124199.0
21	124199.0
22	179111.0
23	234023.0
24	234023.0
25	234023.0
26	306751.0
27	379479.0
28	379479.0
29	379479.0
30	460145.5
31	540812.0
32	540812.0
33	540812.0
34	619681.5
35	698551.0
36	698551.0
37	698551.0
38	767911.0
39	837271.0
40	837271.0
41	837271.0
42	824935.5
43	812600.0
44	812600.0
45	812600.0
46	838172.0
47	863744.0
48	863744.0
49	863744.0
50	789732.0
51	715720.0
52	715720.0
53	715720.0
54	628961.5
55	542203.0
56	542203.0
57	542203.0
58	471008.5
59	399814.0
60	399814.0
61	399814.0
62	333871.0
63	267928.0
64	267928.0
65	267928.0
66	208452.0
67	148976.0
68	148976.0
69	148976.0
70	110555.0
71	72134.0
72	72134.0
73	72134.0
74	50576.0
75	29018.0
76	29018.0
77	29018.0
78	19751.5
79	10485.0
80	10485.0
81	10485.0
82	6975.5
83	3466.0
84	3466.0
85	3466.0
86	2167.5
87	869.0
88	869.0
89	869.0
90	532.0
91	195.0
92	195.0
93	195.0
94	120.5
95	46.0
96	46.0
97	46.0
98	33.0
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3142789984375777
2	0.18129217709178813
3	0.20004654023921448
4	0.22049247339601652
5	0.24588073284108153
6	0.19636921413187597
7	0.1920152600207872
8	0.21669747285324323
9	0.21627090302479193
10	0.22800892795941646
11	0.20054665658981252
12	0.2074159017583208
13	0.21955107791253786
14	0.20900450663669107
15	0.19944345875761096
16	0.20265008712321014
17	0.20278247086307433
18	0.20631270392611928
19	0.20478293626546648
20	0.20088497059168767
21	0.19813433066339847
22	0.2015763078998673
23	0.2057243317489451
24	0.2223752643629739
25	0.215358926150172
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	6798418.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.97447154005062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.02722194961252	45.59692459086148
2	14.605128310604403	17.518697044062627
3	4.688352154787577	8.435443286311276
4	1.9551903794939707	4.690460390813677
5	0.9450411122895432	2.83391706465935
6	0.5065259663149161	1.8227176290630347
7	0.3091238635563242	1.297767825004648
8	0.18919826638653575	0.9077652834260964
9	0.14164312429925865	0.7645474374416766
>10	0.5736167043509308	5.842268400332714
>50	0.031217578062635136	1.2917434970495918
>100	0.02312713165898675	2.681723381139561
>500	0.0028618747241616923	1.1682627638357137
>1k	0.0013075203455888047	1.7311848233754354
>5k	2.7137214658547035E-4	1.2379070063085371
>10k+	1.726913660089357E-4	2.178669576314462
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	38202	0.5619248478101817	No Hit
CCCATGTACTCTGCGTTGATACCAC	36284	0.5337124019146808	No Hit
GAGTACATGGGAAGCAGTGGTATCA	17250	0.2537355014063566	No Hit
ATTCCATTCCATTCCATTCCATTCC	17010	0.25020526834331164	No Hit
CATGTACTCTGCGTTGATACCACTG	14454	0.2126082862218828	No Hit
GAATGGAATGGAATGGAATGGAATG	12739	0.18738182912554066	No Hit
GCGTTGATACCACTGCTTCCCATGT	11310	0.1663622330959938	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	9454	0.13906176407511278	No Hit
GTATCAACGCAGAGTACATGGGAAG	9242	0.13594339153608972	No Hit
GTATCAACGCAGAGTACTTTTTTTT	8900	0.13091280942125064	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	8058	0.11852757509173459	No Hit
TATCAACGCAGAGTACATGGGAAGC	7962	0.1171154818665166	No Hit
ACTCTGCGTTGATACCACTGCTTCC	7839	0.11530623742170606	No Hit
GGTATCAACGCAGAGTACATGGGAA	7665	0.11274681845099845	No Hit
GCTTCCCATGTACTCTGCGTTGATA	6945	0.10215611926186358	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.4709304429354005E-5	0.0
4	0.0	0.0	0.0	2.941860885870801E-5	0.0
5	0.0	0.0	0.0	4.412791328806202E-5	0.0
6	0.0	0.0	0.0	4.412791328806202E-5	0.0
7	0.0	0.0	0.0	7.354652214677003E-5	0.0
8	0.0	0.0	0.0	8.825582657612404E-5	0.0
9	0.0	0.0	0.0	1.1767443543483204E-4	0.0
10	0.0	0.0	0.0	1.4709304429354005E-4	0.0
11	1.4709304429354005E-5	0.0	0.0	1.9122095758160207E-4	0.0
12	1.4709304429354005E-5	0.0	0.0	2.0593026201095606E-4	0.0
13	1.4709304429354005E-5	0.0	0.0	2.0593026201095606E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	195	1.8189894E-12	10.723939	5
CTGTCGC	395	0.0	10.340837	9
TCGGAAC	255	0.0	10.055097	13
TATTCCG	630	0.0	9.807065	5
GAACGAT	285	0.0	9.663512	16
CGAATTA	225	2.7284841E-11	9.285721	15
TAGACAG	955	0.0	9.156947	5
CACGACT	105	4.4690623E-4	9.053143	4
GGAACGA	640	0.0	9.0516	15
GTATTAG	805	0.0	8.979654	1
TAGGACT	700	0.0	8.962611	4
GTCGCCC	350	0.0	8.954352	11
GCACCGA	160	6.7641486E-7	8.905117	6
ACTCGGA	375	0.0	8.863904	11
CGTACCA	500	0.0	8.548785	10
TCTGTCG	445	0.0	8.538553	8
TGTCGCC	435	0.0	8.516031	10
CCTACAC	660	0.0	8.496731	3
CTAGGAC	640	0.0	8.465229	3
GTCTTAG	690	0.0	8.408579	1
>>END_MODULE