FastQCFastQC Report
Wed 25 May 2016
SRR1295043_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295043_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6798418
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC397430.5845918859358162No Hit
GTACATGGGAAGCAGTGGTATCAAC358940.5279757731872327No Hit
GTATCAACGCAGAGTACTTTTTTTT234230.34453603764875884No Hit
GGTATCAACGCAGAGTACTTTTTTT184620.27156317837473365No Hit
GAGTACATGGGAAGCAGTGGTATCA182630.2686360267932922No Hit
ATTCCATTCCATTCCATTCCATTCC164580.24208573229830824No Hit
CATGTACTCTGCGTTGATACCACTG162740.23937922028330708No Hit
TATCAACGCAGAGTACTTTTTTTTT157250.23130381215159174No Hit
GAATGGAATGGAATGGAATGGAATG137850.20276776155864498No Hit
GCGTTGATACCACTGCTTCCCATGT116860.1718929315614309No Hit
ACGCAGAGTACATGGGAAGCAGTGG110070.16190531385389956No Hit
ACGCAGAGTACTTTTTTTTTTTTTT97410.14328333444633737No Hit
GTATCAACGCAGAGTACATGGGAAG96340.14170943887239648No Hit
ACTCTGCGTTGATACCACTGCTTCC88540.13023618141750037No Hit
TATCAACGCAGAGTACATGGGAAGC85990.1264853087880151No Hit
GGTATCAACGCAGAGTACATGGGAA84210.12386705259959008No Hit
GCTTCCCATGTACTCTGCGTTGATA75970.11174658574980237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTC500.001501313.2990075
TTACGCT802.0039133E-613.0615244
TGTACGA951.6465504E-49.9994752
GTCGTAC1054.056315E-59.9642431
CTGATCG800.0045207219.49959
GTCTTAG6850.09.3030451
CCTAGAC5700.09.1659823
TAGACAG10450.09.090235
GTATTAG7900.09.0297361
GTCCGTC950.00182598058.99952711
TACACTG16400.08.8621435
CTAGACT7400.08.8574474
GTTCTAG7200.08.8508141
CTAAGAC6550.08.8466673
GTACCGT1304.377438E-58.7685766
GGAACGA6500.08.76851115
TAAGACT8250.08.7508614
AGTACCG1752.6328598E-78.6850655
TCTATAC7900.08.657583
CTTACAC10300.08.5770293
Produced by FastQC (version 0.11.5)