FastQCFastQC Report
Wed 25 May 2016
SRR1295042_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295042_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3931024
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC258530.6576657888631563No Hit
CCCATGTACTCTGCGTTGATACCAC230180.5855471754942223No Hit
GAGTACATGGGAAGCAGTGGTATCA124560.31686400286541117No Hit
CATGTACTCTGCGTTGATACCACTG91940.23388307982856374No Hit
GCGTTGATACCACTGCTTCCCATGT75240.19140051040136108No Hit
GCTTCCCATGTACTCTGCGTTGATA74210.18878032797561145No Hit
GTATCAACGCAGAGTACATGGGAAG69910.17784170231471494No Hit
ACGCAGAGTACATGGGAAGCAGTGG66690.16965045240120638No Hit
GTATCAACGCAGAGTACTTTTTTTT66580.16937062709360207No Hit
GGTATCAACGCAGAGTACATGGGAA59460.15125829809230368No Hit
TATCAACGCAGAGTACATGGGAAGC58220.14810390371567306No Hit
GGTATCAACGCAGAGTACTTTTTTT57130.14533108930395744No Hit
ACTCTGCGTTGATACCACTGCTTCC51580.13121263060210264No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN45420.11554241337626023No Hit
TATCAACGCAGAGTACTTTTTTTTT43540.11075994448265898No Hit
GTGGTATCAACGCAGAGTACATGGG40800.10378975045687842No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT855.3301577E-511.1759086
TAGACTG4200.09.7316575
TAGACAG5600.09.505345
GTTCTAG4450.09.4036971
TACACCG1054.4701542E-49.0527055
ATTAGAC3350.08.7957613
GTCTTAG5100.08.7646311
GTCTCGC2401.0004442E-108.7180111
CTAGTAC2850.08.6713173
TACACTG7900.08.66309455
TATACTG8200.08.462075
CTACACT6350.08.3836244
GTCTTAT4200.08.3783481
GTCTTGC6950.08.3473951
GTCCTAG4700.08.2964441
GTCCTAA3001.8189894E-128.2424831
GTTCTAC3350.08.2330191
GTTACAC2201.4520992E-88.2089473
TTAGACA4750.08.205554
GTCTAAG2803.274181E-118.1519071
Produced by FastQC (version 0.11.5)